Molasses salt medium

Note YPG media (0.2% yeast extract, 1.0% peptone, and 2.0% glucose) MSM, molasses salt medium (0.2% yeast extract and molasses as carbon source) PDB, potato dextrose broth (20% potato extract and 2% dextrose) YM media (0.3% yeast extract, 0.3% malt extract, 0.5% polypeptone, and 1.0% glucose) AIF, alkali insoluble fraction DA, degree of acetylation DDA, degree of deacetylation DP, degree of polymerization M, molecular weight SmF, submerged fermentation SSF, solid-state fermentation. 

If the needs of the cellular organism are more complex and/or are not very well known, the fermentation requires a complex medium. The C-source, which simultaneously also serves as an N-, S-, and P-source, for such a complex medium can be yeast extract, tryptone, corn steep liquor, or molasses. Requirements for the other elements can be supplemented by the addition of the respective salts. Oxygen is again an exception and has to be supplied in the form of air or, much less often, as pure oxygen. 

The addition of a conunercial fertilizer with salts of nitrogen, phosphorous and potassium (10 10 10) was evaluated. The vinasse appears to have adequate amounts of these components for microbial development, since a decline in their radial growth was observed when these salts were added to the media (Table 2), even when reduced (1/5) doses were evaluated (data not shown). As a control, fertilizer was replaced with the NPK salts from A. nidulans complete medium. Thus in further assays, vinasse medium was standardized as vinasse supplemented with two percent molasses (w v). 

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