Boehringer Mannheim kits

In the analysis of fruit juices, it is important to determine the levels of the individual acids to assess authenticity and quality. A range of these acids can be determined using an enzyme-linked assays and these procedures have been collaboratively tested and published in the IFU compendium of methods (citric no. 22, isocitric no. 54, D-malic no. 64, L-malic no. 21 and D-and L-lactic acids no. 53). r-Biopharm now distributes the Boehringer Mannheim kits to assess the levels of these acids. Similar kits are available from other suppliers. 

Boehringer Mannheim kits are also available for acids such as lactate, acetate, and citrate. In lactate analysis, lactate is oxidized by NAD+ in the... 

Many enzymatic assays have also been developed for the analysis of proteolytic products. Total amino acids in Cheddar cheese were determined by Puchades et al. (1990) using the L-amino acid oxidase enzyme. Glutamic acid has been quantified by flow injection analysis using glutamate dehydrogenase (Puchades et al., 1989) and using the Boehringer-Mannheim kit (McSweeney et al., 1993). 

Starch content was determined using the enzyme-kit of Boehringer/Mannheim. 

AE catalyses the cleavage of acetyl groups from different substrates. The enzyme activity was determined by measuring the release of acetic acid. The amount of acetic acid was measured spectrophotometrically using an acetic acid analysis kit (Boehringer, Mannheim). The activity of AE was measured in 0.6% sugar beet pectin solubilised in 25 mM Na-succinate pH 6.2 and incubated with enzyme fraction in total 500 nl assay. The samples were incubated at 40°C and aliquots were examined after 0, 1, 2 and 3 hours of incubation. The enzyme reaction was stopped by incubating the samples at lOO C for 5 min. Precipitated... 

The molecular masses of polygalacturonase and exopolygalacturonase were approximately determined by gel chromatography on Superose 12 using FPLC device (Pharmacia, Sweden) and the Calibration proteins II kit (Boehringer-Mannheim, Germany). 

Fig. 8. Morphological changes of apoptotic eosinophils induced by dexamethasone (Z2). After eosinophils were treated (a) without or (b) with dexamethasone (2 /u,M) for 12 h, cells were harvested and detected by TUNEL assay using the In Situ Cell Death Detection Kit (Boehringer Mannheim). Briefly, cells were fixed with 4% paraformaldehyde and permeabilized by proteinase K and incubated with the TUNEL reaction mixture containing terminal deoxynucleotidyl transferase (TdT). After washing to remove unbound enzyme conjugated antibody, the horseradish peroxidase retained in the immune complex was visualized by a substrate reaction with diaminobenzidine. The cell nucleus was counterstained with methanol green. Apoptotic eosinophils with nuclear DNA breaks were seen to stain dark brown using a Nikon Eclipse E800 microscope (Nikon Corporation, Tokyo, Japan) in Fig. 8b.

Table IV. Analysis of Milk Products with Tri-enzyme Electrode and Boehringer Mannheim (BM) Test-kit Methods...

The starch content of flours has been similarly analysed with a trienzyme electrode comprising amyloglucosidase, nutarotase and glucose oxidase on nylon mesh and a Boehringer Mannheim starch test-kit. Each sample was pre-incubated with soluble a-amylase for 1 h at room temperature before the actual analysis. Again results obtained with the two techniques were in close agreement (6). 

Glucose in pre-treated ice-creams, drinks, molasses and flour has also been determined with a glucose oxidase based nylon electrode. The results were similar to those obtained by the Yellow Springs Instrument glucose analyser and a Boehringer Mannheim glucose test-kit (10). 

Methods for chemical analysis of NAD(P)H include enzymatic methods [37] and bioluminiscence methods ([38] by Grupe and Gottschalk using a commercial test kit from Boehringer Mannheim). Increased NADH levels often indicate oxygen limited states of aerobic organisms (well known for baker s yeast, and also observed with hybridoma cultures by Zupke et al. [39], where the situation is more complex due to cell compartments). 

Random primer DNA labeling kit (Boehringer-Mannheim, Indianapolis, IN). 

EPS pancreatic amylase test kit from Boehringer Mannheim. Evaluation report 790-1324 527 Sb. 

There are now a number of commercially available kits with which to undertake this assay they can be obtained, for example, from Appligene Oncor (Illkirch, France), Boehringer Mannheim, Pharmingen (San Diego, CA), or Promega (Madison, WI). 

Modifications of the basic method for are well documented for flow cytometry (see Chapter 4) (36), and Kits are also sold by Oncor, Appligene, and Boehringer Mannheim. 

Digoxigenin (Dig) Detection Kit (Boehringer-Mannheim), containing anti-Dig AP conjugate, X-phosphate, and 4-nitro blue tetrazolium chloride (NBT). 

A protocol combining commercial kits for RNA extraction (Amplicor HIV Monitor test, Roche, 073 0246) and one-tube RT-PCR (Titan One-Tube RT PCR system, Boehringer Mannheim, 1 855 476) was established. The procedure described herein is less sensitive than the one described in Subheadings 3.1.1.-3.1.3., because positive amplification results were obtained only starting from 3000 copies/mL. The following protocol was used ... 

Although many aldolases have been characterized for research purposes, these enzymes have not been developed commercially to any significant extent. This is likely due to the availability of the various biocatalysts and the need for dihydroxyacetone phosphate (DHAP) (44), the expensive donor substrate required in nearly all aldolase reactions. A number of chemical and enzymatic routes have been described for DHAP synthesis, which could alleviate these concerns [12], In terms of the enzyme supply issue, this may change with the introduction of products from Boehringer Mannheim and their Chirazyme Aldol reaction kit. They have three kits, each containing a different aldolase fructose-1,6-diphosphate FruA) (EC 4.1.2.13), L-rhamnulose-1-phosphate RhuA (EC 4.1.2.19), and L-fuculose-1-phosphate (FucA) (EC 4.1.2.17). As more screening... 

Screening kits/sets containing samples of the normal commercially available enzymes are also provided by other enzyme suppliers, such as Boehringer Mannheim/Roche (Chirazyme sets for lipases/esterases, aldol reaction kits), Altus Biologies (ChiroScreen Kits TE and EH (based on CLECs, see section 5) for the chiral resolution of alcohols, amines, and esters), Biocatalysts (kits with alcohol dehydrogenases), Enzymatix (lipase biotransformation research kit), and others. 

Nick translation is carried out under conditions found to be optimal for cyanine-modified dUTPs and dCTPs (Biological Detection Systems, Pittsburgh, PA). Similar procedures can be used for dUTPs modified with fluorescein, rhodamine, and coumarin [see instructions with commercial kits sold by Boehringer Mannheim (Indianapolis, IN) and Amersham (Arlington Heights, IL)]. 

In situ Hybridization. MCP-1 cDNA was labeled with DIG-dUTP according to the manufacturer s instructions (DIG labeling and detection kit, Boehringer Mannheim Biochem-ica, Mannhein, Germany) and used as probes for in situ hybridization, which was performed according to a previously... 

The probe can be tested by binding a small amount of the labeled probe (0.5-2 pL) to a nitrocellulose or nylon membrane.The incorporation of label is then detected using an enzymic detection system as described in nonradioactive Southern hybridization detection kits (e.g., Boehringer Mannheim or see Chapter 18, Section 3.2.). 

Bonnichsen and Theorell in 1951 were the first to use ADH for analyzing ethanol in blood, and for several commercial kits, such as Blood Alcohol UV Test BMY (Boehringer Mannheim) and Alcohol Kit (Sigma), are available for measuring ethanol in whole blood, serum, plasma, and urine by using this method. 

Bromodeoxyuridine (BrdU) incorporation is commonly used for the analysis of cell proliferation (Fig. 3B). The explants are labeled by adding BrdU 0.5-3 h before fixation (we use cell proliferation kits from Amersham International, Little Chalfont, UK, or Boehringer-Mannheim, Mannheim, Germany). After fixation in ice-cold methanol, the explants are washed in PBS and immunostained as whole mounts using antibodies against BrdU (12). 

Use cDNA synthesis kit—Boehringer Mannheim ( 1 483 188), Promega ( A3500), or Pharmacia ( 27-9261-01). 

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