Boehringer Mannheim

Technical data, A. M. Usmani and D. Deng, Boehringer Mannheim, IndianapoHs, Ind., 1992. 

Boehringer Mannheim GmbH British Patent 1,369,580 October 9, 1974... 

Gamma-glutamyl-transferase activity was determined according to "Monotest 10 y-GT neu" from Boehringer-Mannheim. 

The concentration of triglycerides was estimated with the use of "Triglycerides GPO-PAP High performance" from Boehringer-Mannheim. 

DE 2 408 804 (Boehringer Mannheim D-prior. 23.2.1974) synthesis of potential metabolites ... 

The purified fungal cutinase fiom F. oxysporum f. sp. pisi was kindly provided by Prof. C.M.J. Sagt in Utrecht University. The commercial product of esterase firom Candida cylindracea was purchased fi om Boehringer Mannheim (Germany). The enzymatic degradation of DEP... 

Starch content was determined using the enzyme-kit of Boehringer/Mannheim. 

AE catalyses the cleavage of acetyl groups from different substrates. The enzyme activity was determined by measuring the release of acetic acid. The amount of acetic acid was measured spectrophotometrically using an acetic acid analysis kit (Boehringer, Mannheim). The activity of AE was measured in 0.6% sugar beet pectin solubilised in 25 mM Na-succinate pH 6.2 and incubated with enzyme fraction in total 500 nl assay. The samples were incubated at 40°C and aliquots were examined after 0, 1, 2 and 3 hours of incubation. The enzyme reaction was stopped by incubating the samples at lOO C for 5 min. Precipitated... 

The molecular masses of polygalacturonase and exopolygalacturonase were approximately determined by gel chromatography on Superose 12 using FPLC device (Pharmacia, Sweden) and the Calibration proteins II kit (Boehringer-Mannheim, Germany). 

Proteins. Standard proteins for column calibration curves were obtained from Boehringer Mannheim. The methods outlined by Latham et al, (15) were followecLto prepare and label crude rat liver nuclear extract with -triiodothyronine. Normal con-... 

Boehringer Mannheim GmbH, Mannheim. Germany. Company literature. 

Neorecormon (epoetin beta, rEPO) Anaemia associated with various medical conditions Boehringer-Mannheim... 

Boehringer Mannheim product insert for anti-digoxigenin-POD, Fab fragments. (1995) Boehringer Mannheim, Hanover, Germany. 

There are cnrrently two sources of monoclonal antibodies to bromodeoxynridine Becton Dickinson Immunocytochemistry Systems and Boehringer Mannheim Biochemicals. Both antibodies have worked eqnally well for the anthor, and both are directed against single-stranded DNA, therefore necessitating the removal of DNA-associated histones and denaturation of the donble-stranded DNA. 

Streptavidin (Boehringer Mannheim, Mannheim, Germany) blocking buffer (1 pg/mL). Biotin-conjugated alkaline phosphatase (Boehringer Mannheim), in blocking buffer, 5 pg/mL. 

Proteinase K (Eluka) or Pepsin (Boehringer Mannheim Biochemicals, Indianapolis, IN) for stock solution 10 mg Proteinase K, 10 mL IX DEPC-treated PBS mix and store 1-mL aliquots in RNase-free Eppendorf tubes at -20°C. 

PCR reaction reagents (Perkin-Ehner and Life Technologies, Inc., Foster City, CA) Ultrapure 100 mM 4(dNTP) stock solution UV-irradiated dH20 Digoxigenin 11-dUTP (Boehringer Mannheim) PCR cocktail, lOX PCR buffer, MgCl2, a set of sense and antisense primers, BSA, Taq polymerase-Taq-start antibody complex (see Note 6). 

Taq start antibody (CLONTECH Laboratories, Palo Alto, CA). Digoxigenin-ll-ddUTP (Boehringer Mannheim). 

Hybridization solntion (Boehringer Mannheim) Deionized formamide 20X SSC lOOX Denhardt solntion 10 mg/mL Salmon sperm DNA 10% SDS. 

Anti-digoxigenin antibody, a Eab fragment (Boehringer Mannheim). 

In the tissue sections, a hybridization is performed with a highly specific probe in order to detect the target (either amplified by indirect in situ RT-PCR or unamplified). For the preparation of nonradioactive digoxigenin-labeled probes, one should refer to the guidelines of the company manuals Genius M System User s guide for membrane hybridization and Nonradioactive in situ hybridization application manual (2nd ed., Boehringer Mannheim). 

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