Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Enzyme linked assays

AP is the second most popular choice for antibody-enzyme conjugation, being used in almost 20 percent of all commercial enzyme-linked assays. Although P-gal and GO are used frequendy in research and cited numerous times in the literature, their utilization for commercial ELISA applications represents less than 1 percent of the total assays available. [Pg.788]

Fig. 20c. 1. ELISA assay, (a) Antibodies to the drug of interest are secured to a solid substratum such as a test tube or micro-well plate. The sample containing the analyte antigen is added to the reaction surface, (b) After the analyte has bound to the antibody, the vessel is rinsed to remove unbound antibody. A second antibody to the analyte is added. This antibody has a bound enzyme which has been chosen because its reaction produces a colored product which can be detected spectrophotometrically. (c) After this second antibody has bound to the first antibody-antigen complex, the surface is again rinsed to remove unbound-antibody enzyme. The enzyme substrate is added in sufficient excess such that the rate of product formed is proportional to the amount of enzyme present. The enzyme-linked assays are very sensitive, since each enzyme can rapidly catalyze thousands of substrate to product reactions. Fig. 20c. 1. ELISA assay, (a) Antibodies to the drug of interest are secured to a solid substratum such as a test tube or micro-well plate. The sample containing the analyte antigen is added to the reaction surface, (b) After the analyte has bound to the antibody, the vessel is rinsed to remove unbound antibody. A second antibody to the analyte is added. This antibody has a bound enzyme which has been chosen because its reaction produces a colored product which can be detected spectrophotometrically. (c) After this second antibody has bound to the first antibody-antigen complex, the surface is again rinsed to remove unbound-antibody enzyme. The enzyme substrate is added in sufficient excess such that the rate of product formed is proportional to the amount of enzyme present. The enzyme-linked assays are very sensitive, since each enzyme can rapidly catalyze thousands of substrate to product reactions.
Piletsky SA, Piletska EV, Chen B, Karim K, Weston D, Barrett G, Lowe P, Turner AP. Chemical grafting of molecularly imprinted homopolymers to the surface of microplates. Application of artificial adrenergic receptor in enzyme-linked assay for beta-agonists determination. Anal Chem 2000 72 4381-4385. [Pg.425]

In the analysis of fruit juices, it is important to determine the levels of the individual acids to assess authenticity and quality. A range of these acids can be determined using an enzyme-linked assays and these procedures have been collaboratively tested and published in the IFU compendium of methods (citric no. 22, isocitric no. 54, D-malic no. 64, L-malic no. 21 and D-and L-lactic acids no. 53). r-Biopharm now distributes the Boehringer Mannheim kits to assess the levels of these acids. Similar kits are available from other suppliers. [Pg.251]

Ironically, AP is the enzyme of choice for some applications due to its stability. Since it can withstand the moderately high temperatures associated with hybridization assays, AP is the enzyme of choice for labeling oligonucleotide probes. Alkaline phosphatase also is capable of maintaining enzymatic activity for extended periods of substrate development. Increased sensitivity can be realized in ELISA procedures by extending the substrate incubation time to hours and sometimes even days. These properties make AP the second most popular choice for antibody—enzyme conjugates (behind HRP), being used in almost 20% of all commercial enzyme-linked assays. [Pg.653]

GO often is used in solution phase chemical reactions as well as being immobilized on dip-sticks and electrodes. Although its overall clinical usage is widespread, its use as conjugated to antibodies in enzyme-linked assay systems is minor compared to the popularity of other enzymes like horseradish peroxidase and alkaline phosphatase. [Pg.654]

Melki, R., Fievez, S., and Carlier, M.-F. (1996). Continuous monitoring of Pi release following nucleotide hydrolysis in actin or tubulin assembly using 2-amino-6-mer-capto-7-methylpurine ribonucleoside and purine-nucleoside phosphorylase as an enzyme-linked assay. Biochemistry 35, 12038-12045. [Pg.295]

Cell preparations are also of use in some instances where the specificity of an enzyme linked assay is not required or the nature of the analyte makes it an unsuitable solution. [Pg.11]

Whatever the market, wherever the application, the development of the Sensor Device requires separate and linked investigation at various levels. Even without a particular final goal, our basic understanding of immunoassay, enzyme-linked assay, recognition proteins, catalytic active sites and their electronic transduction will continue to occupy the field, in addition to more downstream considerations such as life-time levels of detection etc. the list could be unending, these for example, are just some of the considerations ... [Pg.19]

A murine monoclonal antibody has been developed that is specific for conformational changes that occur in Clq after binding to immune complexes. It does not bind native Clq (G14, H19). This antibody (or a similar one) has been used in a solid-phase, enzyme-linked assay for the detection of immune complexes (R3). We have evaluated this test and the monoclonal anti-C3 test with a large panel of specially prepared specimens. We previously used this panel to evaluate five other assays for immune complexes (M10). The monoclonal anti-C3 and anti-Clq assays performed as well as, if not better than, the other assays we evaluated. [Pg.18]

Keywords Enzyme-linked assay M. tuberculosis Mutation detection ... [Pg.170]

Protocol III Detection of ligation on chips via an enzyme-linked assay... [Pg.177]

An enzyme-linked assay was used to detect chip ligation products. [Pg.177]

Brontman, S.B. Meyerhoff, M.E. Homogeneous enzyme-linked assays mediated by enzyme antibodies A new... [Pg.1532]

Figure 8.7. A true solid-phase oligosaccharide library [41], showing exponential increase in number of compounds with each reaction step. The library was screened for active compounds using a solid-phase, enzyme-linked assay and the components were identified using chemical coding. Figure 8.7. A true solid-phase oligosaccharide library [41], showing exponential increase in number of compounds with each reaction step. The library was screened for active compounds using a solid-phase, enzyme-linked assay and the components were identified using chemical coding.
There are also now available commercial enzyme-linked assays for the measurement of mu-class subunits in whole blood and also the quantitation of total alpha-class subunits in plasma (Biotrin International, Dublin). These assays have the advantage of speed over the RIA methods since the results can be obtained in less than a day but few studies have been reported using these methods and their reliability and validity remain to be established. [Pg.325]

Current assays for the detection and analysis of biotin and its analogues either rely on enzyme-linked assays or utihse the high affinity of biotin for avidin (Silver, 1979). [Pg.287]


See other pages where Enzyme linked assays is mentioned: [Pg.56]    [Pg.823]    [Pg.903]    [Pg.965]    [Pg.414]    [Pg.243]    [Pg.480]    [Pg.513]    [Pg.592]    [Pg.141]    [Pg.10]    [Pg.151]    [Pg.750]    [Pg.288]    [Pg.290]    [Pg.292]    [Pg.1577]    [Pg.119]    [Pg.21]    [Pg.304]    [Pg.460]    [Pg.493]    [Pg.572]    [Pg.633]    [Pg.148]    [Pg.126]   
See also in sourсe #XX -- [ Pg.20 ]




SEARCH



Enzymes assay

Linked assay

© 2024 chempedia.info