Amersham International

Radioactive tracers account for about 20% of the worldwide market for consumables and reagents for life science research. In 1994 the value was estimated at about 300 million. The principal fuU line manufacturers are Du Pont—NEN Research Products (Boston, Massachusetts) and Amersham International (Amersham, U.K.). These companies share roughly equaHy about 85% of the radiochemicals worldwide market. In addition to an extensive line of catalog products, these suppHers offer custom labeling and custom synthesis services. The rest of the market is shared by producers of a limited range of products or services, such as ICN Biomedicals (Costa Mesa, California) and American Radiolabeled Chemicals (St. Louis, Missouri). 

Guide to the Self-decomposition ofRaidiochemicals, Amersham International pic, Amersham, U.K., 1992. 

Amersham International Limited, Technical Catalog RS16-8, Mossbauer Sources (1981)... 

Figure 10.17 from Cyclic GMP RJA Kit, Product Information 1976, by permission of Amersham International, U.K. 

Amersham International pic Dept, of Chemistry, University of Surrey, (UK)—a collaboration project (1968) ... 

The Bristol group of Christine Willis, in collaboration with Amersham International, developed a procedure for deuterium (or labeling of nonpolar amino acids." In the chemical steps, a selectively methyl-labeled oxazolidinone is converted first into a 2-methyl carboxylic acid and then lengthened by two carbon atoms without racemization to yield an a-keto methyl ester (Scheme 9). 

Bolton AE (1985) Radioiodination techniques (Amersham Review 18), 2nd ed., Amersham International pic, Amersham Bolton AE, Hunter WM (1973) Biochem J 133 529 Hermanson GT (1996) Bioconjugate techniques. Academic Press, New York,p 414... 

W097/28177 D. Brown, A. Hamilton, D. Loakes, A. Simmonds, and C. Smith (Amersham International), PCTVIO 97/28177. 1997WO97/32880 F. Himmelsbach, G. Dahmann, T. von Riiden, and T. Metz (Thomae GmbH), PCTVIO 97/32880 (1997). 

Carrier-free 125I, radioactive concentration 100 mCi/mL (e.g., code IMS.30, Amersham International, Amersham, UK)... 

ECL western blotting protocols Amersham International pic. Bucks, UK. 

The contributions of an able group of research students - Michael Butters, Karl Fry, Mark Hammond, Anil Mistry, Martin James, Lysanne Pearce, Vincent Boschat and Dennis Jones - and of industrial collaborators - Barry Nay, David Walker, Martin Atkins (BP), Martin Bye (Amersham International), Derek Basset, Paul Ashworth and Janet Chetland (Associated Octel) -are gratefully acknowledged. The companies and the S.E.R.C. are thanked for financial support. 

Some means of checking the efficiency of transfer should be included. We use prestained marker proteins (Rainbow markers, Amersham International), which are visible on the blot after successful transfer. Alternatively, duplicate tracks that are cut off and stained with Amido Black can be included. (Soak in 0.1% (w/v) Amido Black in methanol/ acetic acid/water 45 10 45 for a few minutes and destain in ethanol/ acetic acid/water 90 2 8.)... 

Evans, E.A. (1992) Guide to the Self-Decompositon of Radiochemicals., Chalfont, UK Amersham International pic. 

M13 phage DNA, in the form of SS M13mp8 DNA can be labelled by random primer extension using the Multiprime kit (Amersham International) according to the manufacturer s instructions. 

Amersham Life Science. 1993. Brochure on scintillation. Drug Discovery proximity assays. Publication No. S 593/657/4/93/09. Amersham International. 

The in vitro semi-automated microdilution assay technique that measures the ability of the extracts to inhibit the incorporation of [G- H] hypoxanthine (Amersham International, Buckinghamshire, UK) into the malaria parasite was used. The extracts were tested in duplicate at ten concentrations in two-fold dilutions and the experiment was repeated twice for each extract. Computation of the concentration of drug causing 50% inhibition of [G- H] hypoxanthine uptake (IC50) was carried out by interpolation after logarithmic transformation of both concentration and counts per minute (cpm) values using the formula ... 

Hybridization. Genomic DNA (c. 3 pg)of E. graminis f. sp. horde was digested with the restriction endonucleases Hind III, Sst 1, Pst 1, Xho 1, Sal l, Eco R1 and Bam HI in accordance with the manufacturer s Instructions (BRL), separated by 0.7% agarose gel electrophoresis, and then Southern blotted (45) onto nylon membrane (Hybond-N Amersham International). Prehybridization and hybridization reactions were done at 42°C using 40% deionised formamide, and 5 x SSC and 5 x Denhards solution. 

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