Biotechnology bioproducts

Manufacturing approaches for selected bioproducts of the new biotechnology impact product recovery and purification. The most prevalent bioseparations method is chromatography (qv). Thus the practical tools used to initiate scaleup of process Hquid chromatographic separations starting from a minimum amount of laboratory data are given. 

The application of the SMB-technique to the downstream processing of biotechnological products requires some specific changes to meet the special demands of bioproduct isolation. Some exemplary applications are given including separations of sugars, proteins, monoclonal antibodies, ionic molecules and optical isomers and for desalting. 

Kren V, Harazim P and Malinka Z (1994) Claviceps purpurea (ergot) culture and bioproduction of ergot alkaloids. Biotechnology in Agriculture and Forestry, Vol 58 Medicinal and Aromatic Plants VII (ed Bajaj YPS). Springer, Heidelberg, pp 139-156. 

Approximately 89 million metric t of organic chemicals and lubricants, the majority of which are fossil based, are produced annually in the United States. The development of new industrial bioproducts, for production in standalone facilities or biorefineries, has the potential to reduce our dependence on imported oil and improve energy security. Advances in biotechnology are enabling the optimization of feedstock composition and agronomic characteristics and the development of new and improved fermentation organisms for conversion of biomass to new end products or intermediates. This article reviews recent biotechnology efforts to develop new industrial bioproducts and improve renewable feedstocks and key market opportunities. 

The chemical and lubricant markets represent an enormous opportunity for biobased products, but many obstacles must be overcome on the way to commercialization. To gain acceptance and supplant products made from petroleum, industrial bioproducts will need to be able to compete in terms of both cost and performance advances in biotechnology will play a key role in surmounting these economic and technical barriers. Given the size of the potential markets and the considerable research under way, the future looks bright for a biobased economy. 

Biotechnological transformation is powerful tool to effectively utilize a broad variety of plant oils, with the aim to modify their structure for the production of new lipid-based materials with demanded properties and functions. One method of plant oil transformation is based on the direct utilization by microorganisms. Employed oils can be converted to aimed compounds by submerged cultivation or oils, and/or oleaginous plant materials can be utilized during solid state fermentation to useful bioproducts enriched with demanded microbial products. Another biotransformation technique covers the enzymatic modification of oil components to structured lipids with biological properties. 

Verhoef, S., Ruijssenaars, H.J., de Bont, J.A.M., and Wery, J. 2007. Bioproduction of /7-hydroxybenzoate from renewable feedstock by solvent-tolerant Pseudomonas putida SI 2. Journal of Biotechnology, 132 49-56. 

Kfen V, Harazim P, Malinka Z (1994) Claviceps purpurea (Ergot) Cultivation and ergot alkaloid bioproduction. In Baja) YPS (ed) Medicinal and aromatic plants VII, Biotechnology in. riculture and Forestry (vol 28). Springer, Berlin Heidelberg New York, p 139... 

This book covers several of the emerging areas of separations in biotechnology and is not intended to be a comprehensive handbook. It includes recent advances and latest developments in techniques and operations used for bioproduct recovery in biotechnology and applied to fermentation systems as well as mathematical analysis and modeling of such operations. The topics have been arranged in three sections beginning with product release from the cell and recovery from the bioreactor. This section is followed by one on broader separation and concentration processes, and the final section is on purification operations. The operations covered in these last two sections can be used at a number of different stages in the downstream process. 

Rupp, S. (2013) Next-generation bioproduction systems cell-fiee conversion concepts for industrial biotechnology. Eng. Life Sci., 13, 19-25. 

Xu, J., Dolan, M.C., Medrano, G. et al. (2012) Green factory plants as bioproduction platforms for recombinant proteins. Biotechnology Advances, 30, 1171-1184. 

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