Bacillus subtilis characterization

Pitkanen, K., Heikinheimo, R. and Pakkanen, R. (1992) Purification and characterization of Erwinia chrysanthemi B374 pectin methylesterase produced by Bacillus subtilis. Enzyme Microb TechnoHA, 832-836. 

Berndt C, CH Lillig, M Wollenberg, E Bill, MC Mansilla, D de Mendoza, A Seidler, JD Schwenn (2004) Characterization and reconstitution of a 4Fe-4S adenyl sulfate/phosphoadenyl sulfate reductase from Bacillus subtilis. J Biol Chem 279 7850-7855. 

In the Gram-positive bacterium Bacillus subtilis (DNA with low G + C content), three Fur-like proteins have been characterized (Bsat et al., 1998). One, called Fur, regulates mainly iron uptake and siderophore biosynthesis. A second one, called PerR, regulates peroxide stress response genes and acts with manganese as corepressor. A third one, Zur, regulates genes for zinc uptake. The Zur protein found in E. coli shows only 25 % identity to the B. subtilis Zur, while the two Fur proteins have 32 % identical amino acids. 

Ohshiro, T. Ishii, Y. Matsubara, T., et al., Dibenzothiophene Desulfurizing Enzymes From Moderately Thermophilic Bacterium Bacillus Subtilis WU-S2B Purification, Characterization and Overexpression. Journal of Bioscience and Bioengineering, 2005. 100(3) pp. 266-273. 

Sivendran, S., Patterson, D., Spiegel, E., McGown, I., Cowley, D. and Colman, R. F. Two novel mutant human adenylosuccinate lyases (ASLs) associated with autism and characterization of the equivalent mutant Bacillus subtilis ASL. /. Biol. Chem. 279 53789-53797, 2004. 

BSL 1 Biosafety Level 1 is suitable for work involving well-characterized microorganisms not known to consistently cause disease in healthy adults, and of minimal potential hazard to laboratory personnel and the environment. Safety equipment none required. Microorganisms include Bacillus subtilis, Naegleria gruberi, and infectious canine hepatitis virus. 

Fitzpatrick, T.B., Amrhein, N. and Macheroux, P., Characterization of YqjM, an old yellow enzyme homolog from Bacillus subtilis involved in the oxidative stress response. J. Biol. Chem., 2003, 278, 19891. 

Lampel, K.A., Uratani, B., Chaudhry, G.R., Ramaley, R.F. and Rudikoff, S., Characterization of the developmentally regulated Bacillus subtilis glucose dehydrogenase gene. J. Bacterial., 1986, 166, 238. 

Ito T, Neilands JB (1958) Products of "Low-iron Fermentation" with Bacillus subtilis Isolation, Characterization and Synthesis of 2,3-Dihydroxybenzoylglycine. J Am Chem Soc 80 4645... 

In 1998 the long-known Bacillus subtilis inorganic pyrophosphatase was characterized and found to have much greater activity than the above enzymes, to have a completely different amino acid sequence, not to be inhibited by F and to be activated by This form of... 

Two other structurally characterized transferases have the same or almost the same mode of Mn + coordination as Mn + in Bacillus subtilis glycosyltransferase SpsA described above. A-acetylglucosaminyltransferase I which serves as the gateway from oligomannose to hybrid and complex A-glycans and plays a critical role in mammalian development, has the same active site structure except the Mg + ion and the glycerol are not present. /3 1,3-Glucuronyltransferase I... 

Mukherjee, A. K., Adhikari, H., Rai, S. K. (2008). Rroduction of alkaline protease by a thermophilic Bacillus subtilis under solid-state fermentation (SSF) condition using Imperata cylindrical grass and potato peel as low-cost medium Characterization and application of enzyme in detergent formulation. Biochem. Engg. J.,39, 353-361. 

Kobashi, N. Nishiyama, M. Yamane, H. Characterization of aspartate kinase III of Bacillus subtilis. Biosci. Biotechnol. Biochem., 65, 1391-1394 (2001)... 

Tanaka, T. Kawata, M. Gloning and characterization of Bacillus subtilis iep, which has positive and negative effects on production of extracellular proteases. J. Bacteriol., 170, 3593-3600 (1988)... 

Perego, M. Cole, S.P. Burbulys, D. Trach, K. Hoch, J.A. Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins SpoOA and SpoOF of Bacillus subtilis. J. Bacteriol., 171, 6187-6196 (1989)... 

Arima, K., Kakinuma, A. Tamura, G. (1968). Surfactin, a crystalline peptidolipid surfactant produced by Bacillus subtilis isolation, characterization and its inhibition of fibrin clot formation. Biochemical and Biophysical Research Communication, 31, 488-94. 

LE Quadri, PH Weinreb, M Lei, MM Nakano, P Zuber, CT Walsh. Characterization of Sfp, a Bacillus subtilis phosphopantetheinyl transferase for peptidyl carrier protein domains in peptide synthetases. Biochemistry 37 1585-1595, 1998. 

DN Arnosti, VL Singer, MJ Chamberlin. Characterization of heat shock in Bacillus subtilis. J Bacteriol 168 1243-1249, 1986. 

Characterization of Surfactin from Bacillus subtilis for Application as an Agent for Enhanced Oil Recovery... 

However, the exceptional size-specific behavior of nanomaterials in combination with their relatively large surface-to-volume ratio might result in potential risk for human health and the environment [26-28]. For example, fullerene (C60) particles suspended in water are characterized by antibacterial activity against Escherichia coli and Bacillus subtilis [29] and by cytotoxicity to human cell lines [30]. Single- and multiwalled carbon nanotubes (CWCNTs and MWCNTs) are toxic to human cells as well [31, 32]. Nano-sized silicon oxide (Si02), anatase (Ti02), and zinc oxide (ZnO) can induce pulmonary inflammation in rodents and humans [33-35],... 

Recently, a new SAM dependent lysine 2,3-aminomutase was detected and characterized in Bacillus subtilis. Unlike the enzyme from C. subterminah SB4, the enzyme in B. subtilis apparently consists of four identical subunits each with a molecular mass of 54 kDa [30]. A PLP binding motif was identified in this amino-mutase that is also highly conserved in other lysine 2,3-aminomutases [31]. 

Ebbole, D. J., and Zalkin, H. (1987). Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis. J. Biol. Chem., 262, 8274-8287. 

Merchuk et al. [276] investigated the dynamics of oxygen electrodes when analyzing mass transfer, and they reported whether and when an instantaneous response occurs. A semiempirical description of diffusion coefficients was provided by Ju and Ho [198]. Bacillus subtilis cultures change the product concentration ratio between acetoin and butanediol rapidly in the range of p02 =80-90 ppb [286]. This fact could be used for the characterization of the oxygen transport capabilities of bioreactors. 

Matsunaga I, Ueda A, Fujiwara N, Sumimoto T, Ichihara K (1999) Characterization of the ybdT gene product of Bacillus subtilis novel fatty acid (3-hydroxylating cytochrome P450. Lipids 34 841-846... 

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