Pyrophosphatase inorganic

PROPIONYL-CoA SYNTHETASE PYROPHOSPHATASE, INORGANIC PYROPHOSPHOMEVALONATE DECARBOXYLASE... 

Pyrophosphatase, inorganic Origin baker s yeast Fluka... 

Pyrocatechol dioxygenases, 325 Pyromctallurgy, 780, 832-835 Pyrophosphatase inorganic activation, 581 Pyrophosphates... 

FIGURE 15.20 The adenylyl cyclase reaction yields 3, 5 -cyclic AMP and pyrophosphate. The reaction is driven forward by subsequent hydrolysis of pyrophosphate by the enzyme inorganic pyrophosphatase. 

Davidson, A., Halestrap, A.P. (1989). Inhibition of mitochondrial-matrix inorganic pyrophosphatase by physiological [Ca ], and its role in the hormonal regulation of mitochondrial matrix volume. Biochem. J. 258,817-821. 

This reaction is accompanied by loss of free energy as heat, which ensures that the activation reaction will go to the right and is further aided by the hydrolytic splitting of PP , catalyzed by inorganic pyrophosphatase, a reaction that itself has a large AG of—27.6 kj/... 

A flow assay was reported for determination of inorganic pyrophosphate a pyrophosphatase was coimmobilized with luciferase on Sepharose beads with continuous flow of saturating concentrations of substrates. The instrument allowed automation with a throughput of approximately one sample every 4 min. 

MaeshimaM, Yoshida S. Purification and properties of vacuolar membrane proton-translocating inorganic pyrophosphatase from mung bean. JBiol Chem 1989 264 20,068-20,073. 

Walker RR, Leigh RA. Mg2+-dependent, cation-stimulated inorganic pyrophosphatase associated with vacuoles isolated from storage roots of red beet (Beta vulgaris L.). Planta 1981 133 150-155. 

Vance DH, Czamik AW (1994) Real-time assay of inorganic pyrophosphatase using a high-affinity chelation-enhanced fluorescence chemosensor. J Am Chem Soc 116 9397-9398... 

Inorganic pyrophosphatase Bacillus stearothermophilus 7.9 90 Morita and Mathemeier... 

Salminen, T., A. Teplyakov, J. Kankare, B.S. Cooperman, R. Lahti, and A. Goldman. 1996. An unusual route to thermostability disclosed by the comparison of Thermus thermophilus and Escherichia coli inorganic pyrophosphatases. Protein Sci 5 1014-1025. 

Satoh T, Takahashi Y, Oshida N, Shimizu A, Shinoda H, et al. 1999. A chimeric inorganic pyrophosphatase derived from Escherichia coli and Thermus thermophilus has an increased thermostability. Biochemistry 38(5) 1531. 

NURF ISWI Drosophila Nurf-55, Nurf-301, N urf-38-inorganic pyrophosphatase Although the bulk of Drosophila ISWI does not co-localize with RNA polymerase [233], the NURF complex functions in the activation of transcription [234]. 

Figure 26 Schematic of the coordination sphere in manganese inorganic pyrophosphatase.

In 1998 the long-known Bacillus subtilis inorganic pyrophosphatase was characterized and found to have much greater activity than the above enzymes, to have a completely different amino acid sequence, not to be inhibited by F and to be activated by This form of... 

Enzymes that probably require three metal ions for full activity include the Tetrahymena group I ribozyme, a Mn " -activated bifimctional enzyme with inositol monophosphatase and fructose 1,6-bisphosphatase activities described belowand some endonucleases. " Inorganic pyrophosphatases from E. coli and S. cerevisiae are well characterized both structurally and mechanistically. Both Mg " " and Mn + are activating metal ions and the enzyme from E. coli is most active with just three metal ions in the active site. These enzymes have been described in Section 5.1.8.2.4. 

Inorganic pyrophosphatase [EC 3.6.1.1] plays a central role in phosphorus metabolism by catalyzing the hydrolysis of the phosphoanhydride bond of inorganic pyrophosphate (or, diphosphate PPi). This cleavage reaction acts in conjunction with pyrophosphate-forming ligases to provide an additional thermodynamic impetus for certain biosynthetic reactions. For example ... 

A) Human GalNAc-l-P kinase, (B) human UDP-GalNAc pyrophosphorylase, (C) bovine polypeptide GalNAc-transferase, (D) rabbit creatine kinase and (E) inorganic pyrophosphatase. 

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