Active site structure

Jedrzejas, M. J., Singh, S. Brouillette, W. J. Air, G. M. Luo, M. A. 1995. Strategy for theoretical binding constant, Ki calculation for neuraminidase aromatic inhibitors, designed on the basis of the active site structure of influenza virus neuraminidase. Proteins Struct. Funct. Genet. 23 (1995) 264-277... 

Higushi and co-workers have published the 1.8-A resolution structure of the hydrogenase from D. desulfuricans Miyazaki F 33). For the most part the structure is very similar to that of D. gigas hydrogenase. However, Higushi et al. have provided a radically different interpretation of the active-site structure. Instead of one CO and two... 

As written. Equation 19 Implies a simultaneous loss of two sites of the same type. On a heterogeneous catalyst this is only realistic for adjacent sites, as has recently been suggested by Chien (15). Equation 19 assumes adjacent sites are the same species, which appears consistent with active site structural models appearing in the literature (17-18). Performing the same... 

Figure 3. Schematic depiction of the active site structure on...

Physical studies of the hydroxylase have established the structural nature of the diiron core in its three oxidation states, Hox, Hmv, and Hred. Although the active site structures of hydroxylase from M. tri-chosporium OB3b and M. capsulatus (Bath) are similar, some important differences are observed for other features of the two MMO systems. The interactions with the other components, protein B and reductase, vary substantially. More structural information is necessary to understand how each of the components affects the others with respect to its physical properties and role in the hydroxylation mechanism and to reconcile the different properties seen in the two MMO systems. The kinetic behavior of intermediates in the hydroxylation reaction cycle and the physical parameters of intermediate Q appear similar. The reaction of Q with substrate, however, varies. The participation of radical intermediates is better established with the M. triehosporium... 

The active site of DHFR illustrates several features that are common to enzyme active sites. Some of the salient features of active site structure that relate to enzyme catalysis and ligand (e.g., inhibitor) interactions have been enumerated by Copeland (2000) ... 

The conformational distortions that attend transition state formation involve both steric and electronic changes to the active site structure of the enzyme. These changes can include changes in steric packing forces, van der Waals interactions,... 

We have just discussed several common strategies that enzymes can use to stabilize the transition state of chemical reactions. These strategies are most often used in concert with one another to lead to optimal stabilization of the binary enzyme-transition state complex. What is most critical to our discussion is the fact that the structures of enzyme active sites have evolved to best stabilize the reaction transition state over other structural forms of the reactant and product molecules. That is, the active-site structure (in terms of shape and electronics) is most complementary to the structure of the substrate in its transition state, as opposed to its ground state structure. One would thus expect that enzyme active sites would bind substrate transition state species with much greater affinity than the ground state substrate molecule. This expectation is consistent with transition state theory as applied to enzymatic catalysis. 

Selection of an active-site model almost always leads to truncations of the hydrogen-bond network. Upon optimization of the active-site structure, this may lead to the formation of artificial hydrogen bonds that disrupt the structure. Freezing selected coordinates in the active-site model can prevent some of these hydrogen bonds to form. Another remedy could be to include more residues around the metal center, but larger QM models are much more expensive and there will probably still be truncated hydrogen bonds, although further away from the reaction center. 

Figure 14-7. Snapshots of the active site structures near the transition state of (top) the nucleophilic attack and (bottom) the exocyclic cleavage for the in-line monoanionic O2p mechanism of cleavage transesterification in the hairpin ribozyme. The yellow and red colored cartoon is for the substrate and ribozyme strands, respectively, and water molecules interacting with non-bridging oxygens and O5/ are shown...

Glucose isomerase catalyzes the conversion of D-glucose to D-fructose and has also been used extensively on an industrial scale.1184 Some, but not all, enzymes of this family require Co specifically, while others can function with other divalent ions. Environmental and health issues limit the concentrations of Co in culture media during D-fructose production and other metal ions are being sought as substitutes. Although the active site structure remains unknown, EXAFS, optical and EPR spectroscopy has suggest a low-spin divalent Co ion, bound by N and O-donors only (no S-donors). 

In the blue, Type I copper proteins plastocyanin and azurin, the active-site structure comprises the trigonal array [CuN2S] of two histidine ligands and one cysteine ligand about the copper,... 

The zinc acetate complex of tris(3-/-butyl-5-methylpyrazol-l-yl)borate was prepared as a structural model for carbonic anhydrase and comparison with the enzyme active site structures confirmed that the complexes are excellent structural models.239 A mononuclear zinc hydroxide complex can also be formed with the tris(pyrazolyl) borate ligand system as a structural model for carbonic anhydrase.240... 

Ser/Thr-protein phosphatases are ubiquitous enzymes which constitute the catalytic domains of multiprotein complexes. They are responsible for the dephosphorylation of a range of phosphoproteins. Several protein phosphatases have been characterized by X-ray crystallography and display an active site structure similar to purple acid phosphatase. 

Figure 5. Active site structure of the met form of the E. coli R2 protein of ribonucleotide reductase as determined in a 2.2-A resolution X-ray crystallographic study (14, 102).

Figure 6. The active site structure of the catalytically inactive form of GO (pH 4.5 acetate buffer) as determined in a 1.7-A resolution X-ray crystallographic study (119, 120).

The active site structure of Figure 13.20, with three solvent molecules occupying the opposite face of the triad, is unreactive towards dioxygen. However, structural studies of members of each enzyme family show that formation of an enzyme-substrate complex... 

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