Glucose dehydrogenase gene

Lampel, K.A., Uratani, B., Chaudhry, G.R., Ramaley, R.F. and Rudikoff, S., Characterization of the developmentally regulated Bacillus subtilis glucose dehydrogenase gene. J. Bacterial., 1986, 166, 238. 

M. Wada, K. Kita, H. Yanase, and S. Shimizu, Stereoselective reduction of of ethyl 4-chloro-3-oxobutanoate by Escherichia coli transformant cells coexpressing the aldehyde reductase and glucose dehydrogenase genes, Appl. Microbiol. Biotechnol. 1999, 53, 486-490. 

Kizaki, N Yasohara, Y, Hasegawa, J Wada, M Kataoka, M and Shimizu, S. (2001) Synthesis of optically pure ethyl (S)-4-chloro-3-hydroxyhutanoate by Escherichia coli transformant cells coexpressing the carbonyl reductase and glucose dehydrogenase genes. Appl. Mkorbiol. Biotechnol., 55, 590-595. 

F4. Filosa, S., Cai, W., Galanello, R., Cao, A., De Mattia, D Schettini, F., and Martini, G., A novel single-base mutation in the glucose 6-phosphate dehydrogenase gene is associated with chronic non-spherocytic haemolytic anaemia. Hum. Genet. 94,560-562 (1994),... 

V14. Vulliamy, T. J D Urso, M., Battistuzzi, G Estrada, M., Foulkes, N. S., Martini, G., Calabro, V., Poggi, V., Giordano, R., Town, M., Luzzatto, L., and Persico, M. G Diverse point mutations in the human glucose-6-phosphate dehydrogenase gene cause enzyme deficiency and mild or severe hemolytic anemia. Proc. Natl. Acad. Sci. U.S.A. 85, 5171-5175 (1988). 

Heterologous expression of genes for both the carbonyl reductase and glucose dehydrogenase in E. coli has been achieved as mentioned in the previous section (Kataoka, 1999 Kizaki, 2001) (see Section 19.3). 

Recently, this two-phase reaction was improved by using an Esherichia coli transformant expressing both genes for aldehyde reductase and glucose dehydrogenase as the catalyst. When the E. coli transformant cells were incubated in a two-phase system, 300 mg/ml of the substrate was almost stoichiometrically converted to ethyl (R)-4-chloro-3-hydroxybutanoate (92% e.e.) in 16 h [114]. 

Johansson, H., Sterky, F., Amini, B., Lundberg, J., and Kleczkowski, L.A., 2002, Molecular cloning and characterization of a cDNA encoding poplar UDP-glucose dehydrogenase, a key gene ofhemicellulose-/pectin formation. Biochim. Biophys. Acta 1576 53-58. 

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