Bacillus stearothermophilus

Bacillus stearothermophilus, 11 35, 48 thermal resistance characteristics, 8 634t 

Inorganic pyrophosphatase Bacillus stearothermophilus 7.9 90 Morita and Mathemeier 

Wang et al. report the death kinetics of Bacillus stearothermophilus spores using wet, thermal sterilization. Twenty minutes at 110°C reduces the viable count by a factor of lO". The activation temperature, E/Rg, is 34,200 K. How long will it take to deactivate by a factor of lO  

Kennedy LD (1974) Teichoic-acid synthesis in Bacillus-stearothermophilus. Biochem J 

Biological indicators for steam sterilization utilize bacillus stearothermophilus. In monitoring industrial cycles, a sufficient number of preparations each having a known degree of resistance are added to the load and retrieved after exposure, and cultured. 

Low temperature steam (73°C) and formaldehyde (12mg - ) Bacillus stearothermophilus 5min 

FIG. 3 Three-dimensional model of the protein mass distribution of the S-layer of Bacillus stearothermophilus NRS 2004/3a [(a) outer, (b) inner face]. The square S-layer is about 8 nm thick and exhibits a center-to-center spacing of the morphological units of 13.5 nm. The protein meshwork composed of a single protein species shows one square-shaped, two elongated, and four small pores per morphological unit. (Modified from Ref. 7.) 

Fig. 2.45. Typical D - values (exposure time in which the number of bacteria is reduced by one decade) for Bacillus stearothermophilus for different exposure times and the necessary H2 02 concentrations (table from [2.12]).

Packman, L.C., and Perham, R.N. (1982) Quaternary structure of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus studied by a new reversible cross-linking procedure with bis(imidoesters). Biochemistry 21, 5171-5175. 

Hoxey E.V., Soper C.J. Davies D.J.G. (1984) The effect of temperature and formaldehyde concentration on the inactivation of Bacillus stearothermophilus spores by LTSF. J Pharm 

Zaar A, W Eisenreich, A Bacher, G Fuchs (2001) A novel pathway of aerobic benzoate catabolism in the bacteria AzoarcMS evansii and Bacillus stearothermophilus. J Biol Chem 276 24997-25004. 

The preceding protocol can be successfully applied, essentially without modifications, to prepare active cell-free extracts from bacteria other than Escherichia coli (e.g., Bacillus stearothermophilus and clinical isolates of Pseudomonas aeruginosa bearing multiple antibiotic resistance). 

The cell walls of most Gram-positive bacteria contain (1 ->3) poly(glycerol phosphate) teichoic acids which can bear D-alanyl or glycosyl substituents.56 However, the cell walls of Bacillus stearothermophilus B65 contain a glycerol teichoic acid which is free of substituents.57 This is contrary to evidence that had been obtained from biosynthetic studies.58 

This essential property of IF2 can be tested in at least three different ways, all of which require the availability of f[3H]Met-tRNA and IF2, which are prepared according to the protocol detailed in Milon et al. (2007). However, all the tests described in this section can make use of the sturdier and smaller C domain of Bacillus stearothermophilus IF2, since this domain contains all molecular determinants for the IF2-fMet-tRNA interaction (Guenneugues et al, 2000 Spurio et al, 2000). The method for the preparation and purification of B. stearothermophilus IF2C is essentially that described by Spurio et al. (1993). The concentration of the protein 

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