Cross-linking reversible

Recently, Vasilescu et al. demonstrated the use of formaldehyde to preserve protein interactions in vivo followed by immunoaffinity purification of a targeted complex, cross-link reversal via heating at 95°C, separation by SDS-PAGE, and identification of bands by LC-MS/MS.7 Tagwerker et al. utilized formaldehyde cross-linking in conjunction with a novel tag-based affinity purification method.36... 

Other AR mediators, such as divalent ion chelators, formaldehyde scavenges, such as citraconic anhydride, metal ions, or proteolytic enzymes can enhance AR in certain cases however, their applications are not universal and, in some cases, may even inhibit immunostaining. As noted above, the removal of steric barriers that restrict access of the antibody to its target epitope is a key component of aR." In this context, heating may serve to promote the extraction of diffusible proteins out of the tissue sections following cross-link reversal or proteolytic treatment, opening physical holes or channels in the tissue sections that allow better penetration of antibodies. The physical process of opening holes or channels within the tissue section also likely explains the modest success of ultrasonics as an AR method. ... 

Fig. 10.10 Synthesis of Pd nanoparticles encapsulated in a cross-linked reverse micelle.

As a consequence of the incorporation of ions and solvent molecules into the film, swelling or shrinkage of the polymer matrix takes place. Depending on the nature and the extent of cross-links, reversible elastic deformation or irreversible changes (e.g., dissolution) may occur. Other effects, such as dimerization, ion-pair formation, cross-linking, and so forth, should also be considered. 

Several rubber chemicals have been developed to inhibit or compensate for sulfur cross-link reversion. Several of these are shown in Table 11. 

J. Babin, M. Lepage and Y. Zhao, "Decoration" of shell cross-linked reverse polymer micelles using ATRP A new route to stimuli-responsive nanoparticles. Macromolecules, 41, 1246-1253 (2008). 

H. Gao, M. C. Jones, J. Chen, R. E. Prud homme and J. C. Leroux, Core cross-linked reverse micelles from star-shaped polymers. Chem. Mat., 20, 3063-3067 (2008). 

Babin, J., Lepage, M., and Zhao, Y. (2008) Decoration of shell cross-linked reverse polymer micelles using ATRP a new route to stimuh-responsive nanoparticles. Macmmolecules, 41,1246—1253. 

Jung, H.M., Price, K.E., and Mcquade, D.T. (2003) Synthesis and characterization of cross-linked reverse micelles. Journal of the American Chemical Society, 1255351-5355. 

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