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Bacillus stearothermophilus production

K. Bartsch, R. Schneider, and A. Schulz, Stereospecific production of the herbidde phosphinothridn (glufosinate) purification of aspartate transaminase from Bacillus stearothermophilus, cloning of the corresponding gene, aspC, and application in a coupled transaminase process, Appl. [Pg.202]

Carbohydrase (Bacillus stearothermophilus) Produced as an off white to tan powder or a light yellow to dark brown liquid by controlled fermentation using Bacillus stearothermophilus. Soluble in water, but practically insoluble in alcohol, in ether, and in chloroform. Major active principle a-amylase. Typical applications used in the preparation of starch syrups, alcohol, beer, dextrose, and bakery products. [Pg.148]

K. Hirooka, S. Ohnuma, A. Koike-Take-shita, T. Koyama, T. Nishino, Mechanism of product chain length determination for heptaprenyl diphosphate synthase from Bacillus stearothermophilus, Eur. J. Biochem. 2000, 267(14), 4520-4528. [Pg.93]

The synthesis of amylase in Ps. saccharophila (40) and Bacillus stearothermophilus (24) is stimulated by degradation products of starch as much as or much more than starch itself. But, such relationships could... [Pg.72]

Dry heat is generally used at 180°C for a period of two hours for the sterilisation of glassware, metal instruments and thermostable products. Spore strips of Bacillus stearothermophilus may be placed in the oven as a biological sensor, and removed for culturing at the end of the heating period. Growth indicates that the sterilisation process was inadequate, whilst no growth shows a suitable temperature was maintained for the requisite time. [Pg.127]

Production of a-amylase by Bacillus stearothermophilus (pAT9) and Gene Manipulation to Improve the Stability of the Recombinant Plasmid... [Pg.1]

PRODUCTION OF a-AMYLASE BY BACILLUS STEAROTHERMOPHILUS (pAT9) AND GENE MANIPULATION TO IMPROVE THE STABILITY OF THE RECOMBINANT PLASMID... [Pg.113]

Table 1. Influence of Different Carbohydrates on Biomass and Pullulanase Production of Bacillus stearothermophilus G-82. Table 1. Influence of Different Carbohydrates on Biomass and Pullulanase Production of Bacillus stearothermophilus G-82.
The discovery and chemistry of the non-reducing, chemically stable 2-0-a-D-glucopyranosyl-L-ascorbic acid has been reviewed, and a new synthesis of it has been described in which L-ascorbate and a-cyclodextrin interact in the presence of a cyclomaltodextrin glucanotransferase from Bacillus stearothermophilus. Other products with higher maltooligosaccharides linked to 0-2 of the ascorbate were also formed, but glucoamylase could be used to convert these to more 2-0-a-glucopyranosyl-L-ascorbic acid.7 ... [Pg.191]

Amylases classified in family 13 glycoside hydrolases have been described from the archaea to the bacteria domains. The genus Bacillus includes a set of species able to produce starch-d rading enzymes and has biotechnological potential as a source of these enzymes especially the a-amylases, which are the most fi equent starch-degrading enzymes described for bacteria. In addition. Bacillus a-amylases present activity and are stable in temperatures over 50-60 °C, therefore are very attractive for industrial applications. B. subtilis, Bacillus stearothermophilus. Bacillus lichenifortnis, and Bacillus atnyloliquefaciens have been widely used for commercial production of amylases and for the production of starch derivates. The a-amylases produced by other bacteria and also archaea have to compete with the Bacillus enzymes that already present excellent thermophilic properties and high conversion rates (Prakash and Jaiswal 2010). [Pg.219]

Figure 11 Schematic representation of the formation of mono- and double-layer assembly products as described with S-layer subunits isolated from Bacillus stearothermophilus NRS 2004/3a. This S-layer shows obhque lattice symmetry with center-to-center spacings of the morphological units of 9.4 nm and 11.6 mn and a base angle of 78°. The obhque lattice symmetry allows us to unambiguously determine the orientation of the constituent monolayer sheets in double-layer self-assembly products. On the oblique monolayer sheet A the axes of the two types of small (70 and 100 mn diameter) monolayer cylinders are formed as indicated. One of the axes includes an angle of 24° to the short base vector of the oblique S-layer lattice. The second axis is perpendicular to the Hrst. Both monolayer cyhnders have an identical direction of curvature. Due to differences in the charge distribution on both S-layer surfaces polycationic ferritin (PCF) is only bound to the inner surface of both types of monolayer cylinders. Five types of double-layer self-assembly products with back-to-back orientation of the inner surface of the constituent monolayers have been found. The superimposition of sheets A and B in the double-layer assembly products of type I is demonstrated and the angular displacement of sheet B with respect to A around point X for the assembly products of type 11 to V is indicated. (Modified from Ref. 76.)... Figure 11 Schematic representation of the formation of mono- and double-layer assembly products as described with S-layer subunits isolated from Bacillus stearothermophilus NRS 2004/3a. This S-layer shows obhque lattice symmetry with center-to-center spacings of the morphological units of 9.4 nm and 11.6 mn and a base angle of 78°. The obhque lattice symmetry allows us to unambiguously determine the orientation of the constituent monolayer sheets in double-layer self-assembly products. On the oblique monolayer sheet A the axes of the two types of small (70 and 100 mn diameter) monolayer cylinders are formed as indicated. One of the axes includes an angle of 24° to the short base vector of the oblique S-layer lattice. The second axis is perpendicular to the Hrst. Both monolayer cyhnders have an identical direction of curvature. Due to differences in the charge distribution on both S-layer surfaces polycationic ferritin (PCF) is only bound to the inner surface of both types of monolayer cylinders. Five types of double-layer self-assembly products with back-to-back orientation of the inner surface of the constituent monolayers have been found. The superimposition of sheets A and B in the double-layer assembly products of type I is demonstrated and the angular displacement of sheet B with respect to A around point X for the assembly products of type 11 to V is indicated. (Modified from Ref. 76.)...
The action of a bacterial starch-branching enzyme elaborated by Bacillus stearothermophilus with amylose also produced a nonreducing cyclic a-1 4-D-glucan (cycloamylose) [68]. The product in this reaction had one a-1 6 glyco-... [Pg.258]

Kabaivanova, L., Dobreva, E., Miteva, V. Production of cyclodextrin glucosyltransferase by Bacillus stearothermophilus R2 strain isolated from a Bulgarian hot spring. Journal of Applied Microbiology 1999, 86,1017-1023. [Pg.156]

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Bacillus stearothermophilus

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