Analyte electrophoresis

Bog-Hansen, T.C., Prahl, P., and Lowenstein, H. (1978) A set of analytical electrophoresis experiments to predict the results of affinity chromatographic separations. Fractionation of allergens from cow s hair and dander./. Immunol. Meth. 22, 293. 

Kfivankova, L., Pantiickova, P., Gebauer, P., Bocek, P., Caslavska, J., and Thormann, W. (2003). Chloride present in biological samples as a tool for enhancement of sensitivity in capillary zone electrophoretic analysis of anionic trace analytes. Electrophoresis 24, 505—517. 

If analytical electrophoresis reveals more than one band in the agarose gel (step 4), purify the desired DNA fragment by preparative agarose gel electrophoresis and subsequent elution, using a gel extraction kit. 

Fig. 7.6. Basic design, after Davis (1964), of analytical electrophoresis apparatus for cylindrical gels. The tubes are held in the upper compartment by liquid-tight silicone-rubber grommets. The electrodes are platinum wire (by courtesy of Shandon...

Fig. 7.7. Modified design of cylindrical gel analytical electrophoresis apparatus. One of the design features, which may also be embodied in the circular layout (Fig. 7.6) is that the tubes are immersed in the lower reservoir buffer for better heat dissipation. A further advantage in this system is the presence of a barrier which impedes the diffusion of electrode products into the buffer solution. 

A Karbaum, T Jira. Nonaqueous capillary electrophoresis Application possibilities and suitability of various solvents for the separation of basic analytes. Electrophoresis 20 3396-3401, 1999. 

Abbreviations + = external cooling present A = analytical electrophoresis P = preparative electrophoresis = not used for cell separation intermit. = current intermitted at regular intervals. 

Figure 11 Analysis of a human serum by capillary zone electrophoresis with inherent transient isotachophoretic stacking of analytes. The BGE co-ion, mandelate, acts as terminator in the system where chloride surplus present naturally in the sample acts as the leading type stacker and conditions for transient isotachophoretic stacking of phosphate, citrate, malate, and acetoacetate with mobilities between chloride and mandelate are ensured. BGE is composed of 5 mmol r mandelic acid-h e-aminocaproic acid, pH 3.8. Reprinted with permission from Kfivankova L, Pantuckova P, Gebauer P, et al. (2003) Chloride present in biological samples as a tool for enhancement of sensitivity in capillary zone electrophoretic analysis of anionic trace analytes. Electrophoresis 24 515.)...

See alsa Amperometry. Carbohydrates Sugars -Chromatographic Methods. Derivatization of Analytes. Electrophoresis Principles. Flow Injection Analysis Principles. Ion Exchange Principles. Liquid Chromatography Column Technology Chiral Analysis of Amino Acids. Sensors Amperometric Oxygen Sensors. 

See also. Chemiluminescence Oven/iew. Derivatiza-tion of Analytes. Electrophoresis Oven/iew. Enzymes Oven/iew Immobiiized Enzymes Enzyme-Based Eiec-trodes Enzymes in Physioiogicai Sampies Industriai Products and Processes Enzyme-Based Assays. Fluorescence Clinical and Drug Applications. Immunoassays Overview Production of Antibodies. Immunoassays, Applications Clinical Food Forensic. Immunoassays, Techniques Radioimmunoassays Enzyme Immunoassays Luminescence Immunoassays. Mass Spectrometry Polymerase Chain Reaction Products. Nucleic Acids Chromatographic and Electrophoretic Methods Electrochemical Methods. Polymerase Chain Reaction. 

See also Derivatization of Analytes. Electrophoresis Overview. Extraction Solid-Phase Extraction. Infrared Spectroscopy Near-Infrared. Liquid Chromatography ... 

See also Atomic Absorption Spectrometry Principles and Instrumentation. Atomic Emission Spectrometry Inductively Coupled Plasma. Cosmetics and Toiletries. Derivatization of Analytes. Electrophoresis Is-otachophoresls. Environmental Analysis. Enzymes Overview. Extraction Supercritical Fluid Extraction Solid-Phase Extraction Solid-Phase Microextraction. Ion Exchange Ion Chromatography Applications. Liquid Chromatography Reversed Phase Liquid Chromatography-Mass Spectrometry. Nuclear Magnetic Resonance Spectroscopy - Applicable Elements Carbon-13 Phosphorus-31. Perfumes. 

Zarbl E, Lammerhofer M, Eranco P, Petracs M, Lindner W (2001) Development of stereoselective nonaqueous capUlary electrophoresis system for the resolution of cationic and anphoteiic analytes. Electrophoresis 22 3297—3307... 

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