KINETIC CAPILLARY ELECTROPHORESIS FOR SELECTION, CHARACTERIZATION, AND ANALYTICAL UTILIZATION OF APTAMERS

KINETIC CAPILLARY ELECTROPHORESIS FOR SELECTION, CHARACTERIZATION, AND ANALYTICAL UTILIZATION OF APTAMERS 

Aptamer-target binding is an example of an affinity interaction, a tight and highly selective binding of molecnles (one of which is typically a biopolymer) through a nnmber of weak noncovalent bonds. Dne to the affinity natnre of aptamer-target interaction, affinity methods are nsed for the selection, characterization, and analytical ntilization of aptamers. 

All affinity methods are based on noncovalent binding of a ligand (L) and a target (T), with the formation of a ligand-target complex (L T)  

Aptamers in Bioanalysis, Edited by Marco Mascini Copyright 2009 John Wiley Sons, Inc. 

KCE uses the advantages of CE to create a new analytical platform of kinetic homogeneous affinity methods. Seven KCE methods have been suggested and studied in different applications. The spectrnm of proven applications includes  

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