Polymerase chain reaction-mass spectrometry

Hurst, G. Doktycz, M. Vass, A. Buchanan, M. Detection of bacterial DNA polymerase chain reaction products by matrix assisted laser desorption/ionization mass spectrometry. Rapid. Commun. Mass Spectrom. 1996,10,377-382. 

Krahmer, M. T. Johnson, Y. A. Walters, J. J. Fox, K. F. Fox, A. Nagpal, M. Electrospray quadrupole mass spectrometry analysis of model oligonucleotides and polymerase chain reaction products determination of base substitutions, nucleotide additions/deletions, and chemical modifications. Anal. Chem. 1999, 71, 2893-2900. 

Naito, Y. Ishikawa, K. Koga, Y. Tsuneyoshi, T. Terunuma, H. Arakawa, R. Molecular mass measurement of polymerase chain reaction products amplified from human blood DNA by electrospray ionization mass spectrometry. Rapid Commun. Mass Spectrom. 1995,9,1484-1486. 

Fox, A. Fox, K. F. Castanha, E. Muhammad, W. T. Mass spectrometry polymerase chain reaction products. In Encylopedia of Analytical Science. Amsterdam Elsevier, 2004. 

Additionally it has been our experience that mass spectrometry as a routine detection/identification technique for bacteria is not well received by microbiologists and clinicians who prefer less expensive, less complicated approaches to bacterial typing and identification, such as methods based on polymerase chain reaction (PCR) and enzyme-linked immunosorbent assays (ELISA). For that reason we have adapted our MS approach to serve as a means of biomarker discovery that feeds candidate proteins or leads into development as PCR targets or other immunoassay techniques. 

Wunschel, D. S. Fox, K. F. Bruce, J. E. Muddiman, D. C. Smith, R. D. Analysis of double-stranded polymerase chain reaction products from the Bacillus cereus group by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. Rapid Comm. Mass Spectrom 1996,10, 29-35. 

Finally, several PNA based methods for use in genetic diagnostics have been developed. Of particular interest are methods for fluorescence in situ hybridization (FISH), modulation of polymerase chain reaction (PCR) analyses, and detection of genetic material by sensors and mass spectrometry. 8 ... 

Since then, many novel methods have been developed to analyze gene expression and mutation by using electrochemical techniques [1,2], matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MASS) [3], polymerase chain reaction (PCR) [4,5], bacterial magnetic particles [6], and microbeads [7]. 

MS mass spectrometry ORFs open reading frames PCR polymerase chain reaction... 

Different protein-based methods have been reviewed for species identification in milk and dairy products, and for characterization of cheese maturity, such as electrophoretic, chromatographic, and immunological techniques (11, 12). In addition to new developments in these techniques, the interdisciplinary and dynamic nature of milk product analysis is being enhanced by the application of disciplines already used to analyze other foodstuffs. Among them, capillary electrophoresis (CE), polymerase chain reaction, and isotope ratio mass spectrometry are just gaining popularity for solving dairy authenticity problems (13-15). 

DASH, dynamic allele-specific hybridization AS-PCR, allele-specific polymerase chain reaction AS-PE, allele-specific primer extension APEX, arrayed primer extension FP-TDI, fluorescence polarization template directed dye terminator incorporation MALDI-TOF-MS, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry OLA, oligonucleotide ligation assay RCA, ... 

See also Capillary Electrophoresis Clinical Applications. Forensic Sciences DNA Profiling. Lab-on-a-Chip Technologies. Mass Spectrometry Polymerase Chain Reaction Products. Nucleic Acids Chromatographic and Electrophoretic Methods. 

MASS SPECTROMETRY/Polymerase Chain Reaction Products 509... 

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