Analyte bands, capillary electrophoresis

Analyte, 1,2,9 atoms, 221,239,245 bands, capillary electrophoresis, 873,880 concentration standard, 12 unknown, 11 deposition, 749 emission, 2.50 fluorescence, 419 fluorescent in capillary... 

Capillary zone electrophoresis, an up-to-date high resolution separation method useful for proteins and peptides, has been shown to be a useful method for determining electrophoretic mobilities and diffusion coefficients of proteins [3], Diffusion coefficients can be measured from peak widths of analyte bands. The validity of the method was demonstrated by measuring the diffusion coefficients for dansylated amino acids and myoglobin. 

The first approach to interfacing CE with MS was reported by Smith et al.48 when they incorporated the electrospray ionization (ESI) technique introduced by Dole et al.66 This development was based on the recognition that it is not necessary for the detection end of the CE capillary to be immersed in the buffer reservoir as conventionally practiced, as long as it is biased negative of the cathode potential (assuming a cathodic detector end). The ESI was created directly at the terminus of the CE capillary, avoiding any postcolumn region that would contribute to extracolumn band spread or analyte adsorption. A quadrupole mass filter was combined with ESI to produce the first on-line MS detection with capillary electrophoresis. 

Capillary electrophoresis (CE) is a routine analytical technique for fast and efficient separation of charged species. Under the influence of an electric field, the ionic species in a sample that is introduced as a plug (or zone) into an electrolyte at one end of a capillary will be separated into discrete bands when they migrate to the other end of the capillary at different electrophoretic velocities. However, Joule heating is an inevitable phenomenon in CE that limits the performance of electrophoretic separation. 

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