Electrophoretic analysis

The terminal dUTP nick end labeling assay (TUNEL reaction) and electrophoretic analysis of DNA/organotin(IV) mixtures allowed the investigation of DNA fragmentation. 

The need to develop new materials for electrophoretic analysis and macromolecular separations prompted by the needs of the human genome project and the rapidly advancing fields associated with biotechnology, advances in the development of new analytical instrumentation—especially capillary electrophoresis, and practical limitations of the media currently used for gel electrophoresis [73]... 

Richards, EG Temple, Cl, Some Properties of Polyacrylamide Gels, Nature 230, 92, 1971. Righetti, PG, On the Pore Size and Shape of Hydrophilic Gels for Electrophoretic Analysis In Electrophoresis 81 Walter de Gmyter Berlin, 1981 3. 

Conkle, M. T., Schiller, G. and Grunwald, C. 1988. Electrophoretic analysis of diversity and phytogeny of Pinus brutia and closely related taxa. Syst. Bot. 13 411-424. 

Kuo, K.L., Huang, H.Y., and Hsieh, Y.Z., High-performance capillary electrophoretic analysis of synthetic food colorants, Chromatographia, 47, 249, 1998. 

Nielsen, R. G. and Rickard, E. C., Method optimization in capillary zone electrophoretic analysis of hGH tryptic digest fragments, /. Chromatogr, 516, 99 1990. 

Okemgbo A.A., Hill H.H., Metcalf S.G., and Bachelor M., Metal ion interferences in reverse polarity capillary zone electrophoretic analysis of Hanford Defense Waste for ethylenediaminetetraacetic acid (EDTA) and N-hydroxy-ethylethylenediaminetriacetic acid (HEDTA), Anal. Chim. Acta, 396,105,1999. 

Another attempted synthesis of Tc(III)-EDTA and Tc(III)-HEDTA complexes (EDTA ethylenediaminetetraacetic acid HEDTA A -(2-hydroxy-methyl)ethylenediamine-N,AT, iV -triacetic acid) was carried out using [Tc(tu)6]3+ as the starting complex [40]. Technetium-EDTA complexes have been synthesized by the direct reduction of pertechnetate with a suitable reduc-tant in the presence of excess EDTA [41-43]. On addition of EDTA to the Tc(tu) + solution, the intensity of the absorption spectrum decreased with time and the solution color changed from reddish orange to light brown. An electrophoretic analysis for the Tc(III)-EDTA complex showed that more than 70%... 

Hiraoka, A., et al (1998). Sodium dodecyl sulfate-capillary gel electrophoretic analysis of molecular mass microheterogeneity of beta-trace protein in cerebrospinal fluid from patients with central nervous system diseases./. Chromatogr. A 802, 143-8. 

Volume 212. DNA Structures (Part B Chemical and Electrophoretic Analysis of DNA)... 

Gianazza, E. and Arosio, P. (1980) Electrophoretic analysis of horse tissue ferritins at different pH values. Biochimica et Biophysica Acta, 625, 310-317. 

One concern relating to SDS-PAGE-based purity analysis is that contaminants of the same molecular mass as the product will go undetected as they will co-migrate with it. Two-dimensional electrophoretic analysis would overcome this eventuality in most instances. 

WAGONER, W LOSCHKE, D.C., HADWIGER, L.A., Two-dimensional electrophoretic analysis of in vivo and in vitro synthesis of proteins in peas inoculated with compatible and incompatible Fusarium solani, Physiological Plant Path., 1982,20,99-107. 

Electrophoresis is normally run in aqueous media, hence the analytes must be soluble in water. Presently only three types of water-soluble dendrimers have been successfully analyzed using gel electrophoresis techniques. The list includes Starburst PAMAM dendrimers [21], nucleic acid dendrimers [21] and poly(lysine) dendrimers [23, 24] (see Figures 10.2, 10.4 and 10.6). However, in each case appropriate water solubilizing terminal groups are required (i.e. -NH2, -OH or C02H groups) for suitable electrophoretic analysis. 

PAMAM dendrimers provide a series of nearly precise macromolecules with almost equivalent charge density, yet a wide molecular weight range (a few hundred to almost a million), of which gel electrophoretic analysis can be carried out under native conditions [21],... 

Results of electrophoretic analysis of proteins in the allantoic fluid and blood serum before and after photodynamic treatment are presented in Figs. 5.5 and 5.6. Visually, we did not detect any changes in the position and intensity of protein bands. In order to quantitatively analyze these parameters we scanned the gels and measured the relative optical density of the bands (Figs. 5.7 and 5.8). 

In native gel electrophoresis and CZE, the sample components are resolved by their differences in electrophoretic mobility or mass-to-charge ratios. Electrophoretic analysis under native conditions in gel electrophoresis is not as widely used as SDS-PAGE. In gels, disadvantages of native analysis are the low field... 

Righetti, P. G. (2001). Capillary electrophoretic analysis of proteins and peptides of biomedical and pharmacological interest. Biopharm. Drug Dispos. 22, 337—351. 

Jouyban, A., Ye, J., and Clark, B. J. (2002). Non-aqueous capillary electrophoretic analysis of hexetidine in a commercial liquid formulation. Pharmazie 57, 248—249. 

Kfivankova, L., Pantiickova, P., Gebauer, P., Bocek, P., Caslavska, J., and Thormann, W. (2003). Chloride present in biological samples as a tool for enhancement of sensitivity in capillary zone electrophoretic analysis of anionic trace analytes. Electrophoresis 24, 505—517. 

Knops, L. A., Northrop, D. M., and Person, L. C. (2006). Capillary electrophoretic analysis of phosphorous species in clandestine methamphetamine laboratory samples. J. Forensic Sci. 51, 82—86. 

Traditional sample preparation conditions to form SDS-protein complexes prior to electrophoretic analysis included heat treatment at elevated temperatures (e.g., In the case of non-reduced rMAbs, this could lead to sample preparation artifacts in the form of thermally induced fragmentation attributed to disulfide reduction and exchange reactions. Moreover, it was reported that high pH (>9.0) also enhanced the... 

The ProteomeLab PA800 instrument was set up to analyze a sequence of five different labeled rMAb samples with a total of 10 duplicate injections. On many occasions, the electrophoretic analysis of approximately 60% of the injections did not take place due to pressure failures in the instrument. Upon closer inspection, some of red rubber caps of the gel matrix-containing vials were missing, electrodes were bent, and capillaries were broken. Additionally, it was observed that the interface block was coated with a sticky film of gel matrix. It was apparent that the build-up of gel buffer was causing the red rubber caps to remain stuck to the interface block and interfere with the contact of vials in subsequent steps. To improve the removal of gel buffer, the interface block and the surrounding areas were carefully flushed with deionized water. Then, cotton swabs were used to wipe the grooves on... 

Suzuki, S., Ishida, Y., Arai, A., Nakanishi, H., and Honda, S. (2003). High-speed electrophoretic analysis of l-phenyl-3-methyl-5-pyrazolone derivatives of monosaccharides on a quartz microchip with whole-channel UV detection. Electrophoresis 24, 3828—3833. 

Dickerson RE (1992) In Lilley DM, Dahlberg JE (eds) Methods in enzymology, vol 112 DNA structures. Part B. Chemical and electrophoretic analysis of DNA, Academic Press, New York... 

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