Adhesion of Pseudomonas

Gu J-D, Belay B, Mitchell R (2001) Protection of catheter surfaces from adhesion of Pseudomonas aeruginosa by a combination of silver ions and lectins. World J Microbiol Biotech 17 173-179... 

M. Ramstedt and H. J. Mathieu, Ways to decrease the adhesion of Pseudomonas Aeruginosa bacteria to the surfaces of endotracheal tubes, ECASIA 05 Book of Abstracts, Vienna, 2005, p. 76. 

Figure 4. Adhesion of Pseudomonas NCMB-2021 os. surface energy of solid...

Recently, WPCs have been used as carriers for biopolymers and microorganisms (Fig. 26.4). Robledo-Ortiz et al. [50] used a composite material of recycled HDPE and agave fibers for bacterial immobilization. According to the results reported, the natural adhesion of Pseudomonas putida FI onto the composite surface is strongly affected by temperature, pH, ionic strength, and initial biomass concentration. Vdzquez et al. [51] coated the same material (agave fibers/HDPE) with chitosan to be applied in heavy-metal adsorption. These studies showed that composite materials represent an attractive low-cost recycled support for bacterial and biopolymers with potential applications in biotechnological and environmental cleanup processes. 

Ko, H. L., Beuth, J., Solter, J., Schroten, H., Uhlenbruck, G., and Pulverer, G., 1987, In vitro and in vivo inhibition of lectin mediated adhesion of Pseudomonas aeruginosa by receptor blocking carbohydrates. Infection 15 237-240. 

DeFlaun, M. F., Tanzer, A. S., McAteer, A. L., Marshall, B. Levy, S. B. (1990). Development of an adhesion assay and characterization of an adhesion-deficient mutant of Pseudomonas fluorescent. Applied and Environmental Microbiology, 56, 112—19-... 

Reid, G. Sharma, S. Advikolanu, K. Tieszer, C. Martin, R.A. Bruce, A.W. Effects of ciprofloxacin, norfloxacin, and ofloxacin on in vitro adhesion and survival of pseudomonas aeruginosa AKl on urinary catheters. Antimicrob.Aigents Chemothen, 1994, 38, 1490-1495 [column temp 40]... 

Vieira, M.J., Oliveira, R., Melo, L.F., Pinheiro, M.M. and Martins, V., 1993, Effect of metallic ions on the adhesion of biofilms formed by Pseudomonas fluorescens. Colloids and Surfaces B Biointerfaces, 1, 119 - 124. 

In a further study of Pseudomonas NCMB 2021, Fletcher and Loeb (34) compared attachment to a variety of low energy surfaces. Their clata, together with data from Zisman (2. 35). Metsik (36) and Mittal (13) is plotted in Figure 4. This curve is very sTmi-lar to curves relating c or Wg to adhesive strength for plastics (Dyckerhoff and Sell, ) and metals (Kitazaki and Hata, 1J[) The... 

Plant and other lectins have the ability to adhere strongly to microbial cell surfaces and lectins are responsible for the adhesion of Rhizobim trifotii to root hair cells of clover. Discovery of lectins which will bind other microbes may lead to the use of lectins as bridging agents to bind cells to inert support materials. Fletcher (83) studied the effects of proteins on the adhesion of a marine Pseudomonas sp. to polystyrene Petri dishes. Bovine serum albumin, gelatin, fibrinogen and pepsin (pKj — 5.8) all inhibit attachment at pH 7.6 when present either prior to or concurrently with the microbial cells. Bovine serum albumin decreased the adhesion of previously attached cells. 

A wide variety of natural and synthetic materials have been used for biomedical applications. These include polymers, ceramics, metals, carbons, natural tissues, and composite materials (1). Of these materials, polymers remain the most widely used biomaterials. Polymeric materials have several advantages which make them very attractive as biomaterials (2). They include their versatility, physical properties, ability to be fabricated into various shapes and structures, and ease in surface modification. The long-term use of polymeric biomaterials in blood is limited by surface-induced thrombosis and biomaterial-associated infections (3,4). Thrombus formation on biomaterial surface is initiated by plasma protein adsorption followed by adhesion and activation of platelets (5,6). Biomaterial-associated infections occur as a result of the adhesion of bacteria onto the surface (7). The biomaterial surface provides a site for bacterial attachment and proliferation. Adherent bacteria are covered by a biofilm which supports bacterial growth while protecting them from antibodies, phagocytes, and antibiotics (8). Infections of vascular grafts, for instance, are usually associated with Pseudomonas aeruginosa Escherichia coli. Staphylococcus aureus, and Staphyloccocus epidermidis (9). 

Desai and Hubbell (34) have entrapped PEO chains to the surface by partially dissolving the base polymer in a suitable solvent Poly(ethylene terephthalate) (PET) with entrapped PEO (Mol. Wt 18,5(X)) decreased albumin adsorption by 80% and platelet adhesion by more than 95% as compared to the control PET. In addition, the adhesion of Staphylococcus epidermidis. Staphylococcus aureus, and Pseudomonas aeruginosa was r uced by 70-95% on PEO-modified PET compared to control PET... 

In practical cell culture applications, fluid flow methods of estimating cell adhesion have been most useful. In particular, the radial flow chamber shown in Fig. 12.14 was developed to study both deposition and removal of cells at surfaces. The apparatus has been used to measure adhesion of many cell types to different surfaces, including diatoms and pseudomonas fluorescens. ... 

Damron FH, Napper J, Teter MA, et al. Lipotoxin F of Pseudomonas aeruginosa is an AlgU-dependent and alginate-independent outer membrane protein involved in resistance to oxidative stress and adhesion to A549 human lung epithelia. Microbiology. 2009 155 1028-38. doi 10.1099/mic.0.025833-0. 

An early study utilizing Limulus amoebocyte lysate indicated a PTFE surface containing the least number of bacteria per unit area followed by the increasing bacterial adhesion order of FEP, PVDF, PVF, polyethylene, and glass. Another study " ] focused on the adhesion of Staphylococcus epidermidis. Staphylococcus aureus, Escherichia coli, Klebsiella aerogenes, and Pseudomonas aeruginosa adhesion to intravascu-... 

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