In vitro adhesion

Nicholls, L., Grant, T. H., and Robins-Browne, R. M. (2000). Identification of a novel genetic locus that is required for in vitro adhesion of a clinical isolate of enterohaemorrhagic Escherichia coli to epithelial cells. Mol. Microbiol. 35, 275-288. 

Pimienta C, Chouinard F, Labib A, Lenaerts V (1992) Effect of various poloxamer coatings on in vitro adhesion of isohexylcyanoacrylate nanospheres to rat ileal segments under liquid flow. Int J Pharm 80 1-8... 

Walsh, G.M., Wardlaw, A.J., Hartnell, A., Sanderson, C.J. and Kay, A.B. (1991a). Interleukin-5 enhances the in vitro adhesion of human eosinophils, but not neutrophils, in a leucocyte integrin (CDll/l8)-dependent manner. Int. Arch. Allergy Appl. Immunol. 94, 174-178. 

Reid, G. Sharma, S. Advikolanu, K. Tieszer, C. Martin, R.A. Bruce, A.W. Effects of ciprofloxacin, norfloxacin, and ofloxacin on in vitro adhesion and survival of pseudomonas aeruginosa AKl on urinary catheters. Antimicrob.Aigents Chemothen, 1994, 38, 1490-1495 [column temp 40]... 

As an alternative approach to blending, natural and synthetic polymers can be combined in fibrous scaffolds fabricated by core—shell techniques. Chen et al. (2010) prepared nanofibrous scaffolds by core—shell electrospinning based on a commercial thermoplastic PUR and collagen as the fibre core and shell, respectively. The collagen shell was cross-linked by glutaraldehyde vapours. The electrospun mats showed an intermediate mechanical behaviour as compared to the pure component electrospun membranes moreover, the core—shell electrospun membranes were found to support in vitro adhesion and proliferation of pig iliac endothelial cells. 

The preparation of a bioadhesive drug delivery system based upon gelatin/polyacrylic acid microspheres loaded with a model drug (oxprenolol hydrochloride) is described. The effects of preparation and formulation variables on the physicochemical properties and pharmaceutical characteristics of the microspheres are examined and the in-vitro adhesion, bioadhesion, drug release and drug bioavailability behaviour of the drug delivery system evaluated. 15 refs. 

BA 233 (7) and BA 433 (8) were compared as regards their inhibitory action on ADP and noradrenaline-induced platelet aggregation and adhesiveness in vitro. Adhesiveness and aggregation tiere markedly inhibited by 7 auid 8 at 5-50 ig/ml while dipyridamole at 50-100 ig/ml. showed only a treak and Irregular effect ... 

Ahearn, D. G., Grace, D. T., Jennings, M. J., Boraqani, R. N., Boles, K. J., Rose, L. J., Simmons, R. B. and Ahanotu, E. N., 2000. Effects of hydrogel/silver coatings on in vitro adhesion to catheters of bacteria assosiated with urinary tract infections. Current Microbiology 41, 120-125. 

Ouwehand, A.C., Salminen, S. 2003. In Vitro Adhesion Assays for Probiotics and their in vivo Relevance A Review. Microb EcolHealth Disease 15, 175-184. 

Caballo, J., Ferreiros, C. M., and Criado, M. T., Influence of Blood Proteins in the in vitro Adhesions of Staphylococcus Epidermidis to Teflon , Polycarbonate, Polyethylene and Bovine Pericardium, Revista Espanola De Fisiologia, 47(4) 201-208,1991. 

Fibrinogen has been rapidly photo-cross-Unked in the presence of ruthenium as the trisbipyridyl complex and the oxidant ammonium or sodium persulfate by light to yield high-molecular-weight products that have strong in vitro adhesive properties [73]. A mixture of fibrinogen (150 mg/mL), 2 mM Ru (bpy)3Cl2, and 20 mM sodium persulfate was prepared, and applied immediately to wounds, followed by... 

While the in vitro adhesion can partly be described by theoretical models, the in vivo situation m.ay be completely different. Both, polymers and bacteria, undergo changes in the dynamic environment of the living organism, e.g. by protein adsorption to their surfaces, and this may affect the adhesion process. It is very likely that under in vivo conditions specific interactions between bacterial structures and the polymer may overrule the non-specific forces which regulate the adhesion in vi tro. 

In a more recent study on adhesion of two different 5. epidermidis strains (KH 6 and KH 11) onto polyetherurethanes, the importance of hydrophobic interactions was confirmed the more hydrophobic strain KH 6 adheres stronger to the polyetherurethanes BIOMER and TUFTANE which have quite similar surface free energies.37 similar results, stressing the role of hydrophobic bonding in in vitro adhesion, were found by other... 

Lejoly-Boisseau H, Appriou M, Seigneur M, Pmvost A, Tribouley-Duret J, Tribouley J (1999). Schistosoma mansoni in vitro adhesion of parasite eggs to the vascularendothelium. Subsequent inhibition by a monoclonal antibody directed to acarbohydrate epitope. Exp Parasitol 91 20 9. 

Collado, M.C., Grzeskowiak, L., and Salminen, S. (2007) Probiotic strains and their combination inhibit in vitro adhesion of pathogens to pig intestinal mucosa. Curr Microbiol 55, 260-265. 

Early investigations of serum lipids [191, 192] could not establish any difference between MS and controls. More recently a low level of linoleate in the fatty acids of triglycerides and cholesterol esters [193, 194] as well as in the phospholipids of platelets and RBC, have been reported. The C18 2 deficit in triglycerides and cholesterol esters increases with progression of the disease while the proportion of C18 2 in phospholipids of platelets and RBC parallels that in the serum and incre2ises after intake of sunflower seed oil [195]. The proportion of arachidonic acid in these three blood components, however, is similar in MS and control samples. An interrelation between these findings and increased in vitro adhesiveness of blood platelets [196, 197] could not be established [195, 198]. 

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