Adhesion Pseudomonas aeruginosa

Gu J-D, Belay B, Mitchell R (2001) Protection of catheter surfaces from adhesion of Pseudomonas aeruginosa by a combination of silver ions and lectins. World J Microbiol Biotech 17 173-179... 

Keywords Biomaterials surface functionalization, XPS, Tof-SIMS, photo-immobilization, glycoengineering, bacterial adhesion, endotracheal tubes, PS biosensors fluorescence immunoassays, AgN03, PVC, Pseudomonas aeruginosa... 

Pseudomonas aeruginosa bacterial adhesion on PVC endotracheal tubes can be reduced either by rf plasma modification or wet chemical treatment using AgNO . 

D. J. Balazs, Surface Modification and Characterization of Endotracheal Tube Surfaces to Reduce Pseudomonas Aeruginosa Adhesion Plasma treatment, Plasma Polymerization and Chemical Methods (PhD Thesis EPFL no. 2748, 2003). 

M. Ramstedt and H. J. Mathieu, Ways to decrease the adhesion of Pseudomonas Aeruginosa bacteria to the surfaces of endotracheal tubes, ECASIA 05 Book of Abstracts, Vienna, 2005, p. 76. 

The carbohydrate binding of H. pylori is still a complex situation with many postulated receptors. The picture is further confounded by the use of different strains, phase-variation, and use of different methods. The complex carbohydrate recognition pattern identified might partly be a result of the intense research efforts directed towards H. pylori. A parallel case is the multiple adhesive mechanisms described for Pseudomonas aeruginosa, another bacterium subjected to intense studies [65, and references therein]. 

Reid, G. Sharma, S. Advikolanu, K. Tieszer, C. Martin, R.A. Bruce, A.W. Effects of ciprofloxacin, norfloxacin, and ofloxacin on in vitro adhesion and survival of pseudomonas aeruginosa AKl on urinary catheters. Antimicrob.Aigents Chemothen, 1994, 38, 1490-1495 [column temp 40]... 

A wide variety of natural and synthetic materials have been used for biomedical applications. These include polymers, ceramics, metals, carbons, natural tissues, and composite materials (1). Of these materials, polymers remain the most widely used biomaterials. Polymeric materials have several advantages which make them very attractive as biomaterials (2). They include their versatility, physical properties, ability to be fabricated into various shapes and structures, and ease in surface modification. The long-term use of polymeric biomaterials in blood is limited by surface-induced thrombosis and biomaterial-associated infections (3,4). Thrombus formation on biomaterial surface is initiated by plasma protein adsorption followed by adhesion and activation of platelets (5,6). Biomaterial-associated infections occur as a result of the adhesion of bacteria onto the surface (7). The biomaterial surface provides a site for bacterial attachment and proliferation. Adherent bacteria are covered by a biofilm which supports bacterial growth while protecting them from antibodies, phagocytes, and antibiotics (8). Infections of vascular grafts, for instance, are usually associated with Pseudomonas aeruginosa Escherichia coli. Staphylococcus aureus, and Staphyloccocus epidermidis (9). 

Desai and Hubbell (34) have entrapped PEO chains to the surface by partially dissolving the base polymer in a suitable solvent Poly(ethylene terephthalate) (PET) with entrapped PEO (Mol. Wt 18,5(X)) decreased albumin adsorption by 80% and platelet adhesion by more than 95% as compared to the control PET. In addition, the adhesion of Staphylococcus epidermidis. Staphylococcus aureus, and Pseudomonas aeruginosa was r uced by 70-95% on PEO-modified PET compared to control PET... 

As a result of hospital infections caused by the increased resistance of bacterial strands to antibiotics inducing infections for which they are not effective, such as methiciUin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, and certain strands of Escherichia coli, there is a need to develop new robust antibacterial films.These films should reduce or abate pathogenic bacteria resistant to synthetic antibiotics when administered for a long time. The films should have fast bacterial reduction kinetics, be uniform, adhese to the support, and have low cytotoxicity. That is the focus of this study addressing silver (Ag)-nitride/oxynitride bacterial inactivation films. Nitrides/oxynitrides have been shown to be resistant composites and have no or extremely low cytotoxic activity. 

Damron FH, Napper J, Teter MA, et al. Lipotoxin F of Pseudomonas aeruginosa is an AlgU-dependent and alginate-independent outer membrane protein involved in resistance to oxidative stress and adhesion to A549 human lung epithelia. Microbiology. 2009 155 1028-38. doi 10.1099/mic.0.025833-0. 

A decrease of 0/C and P/C concentration ratios at the surface of Streptococcus sobrims was reported as a result of preincubation with polyclonal antibodies. A combination of the pH-dependent zeta potential and the XPS data suggested that polyclonal antibody adsorption occurred through blocking of surface phosphate. XPS measurements provided evidence for IgG attachment to Pseudomonas aeruginosa. The antibody-modified cells showed a reduced adhesion to glass under flow in a nutrient medium. ... 

An early study utilizing Limulus amoebocyte lysate indicated a PTFE surface containing the least number of bacteria per unit area followed by the increasing bacterial adhesion order of FEP, PVDF, PVF, polyethylene, and glass. Another study " ] focused on the adhesion of Staphylococcus epidermidis. Staphylococcus aureus, Escherichia coli, Klebsiella aerogenes, and Pseudomonas aeruginosa adhesion to intravascu-... 

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