A549 cells

The toxicity is not only based on the amount or size of silica nanoparticles, but also on the cell line [95]. Cancer cell lines (A549, MKN-28) had a higher viability and resistance to silica nanoparticles than did normal cell lines (MRC-5, WS1 and CCD-966sk) [111]. Similarly, a previous study showed that A549 cells were more resistant to the treatment of silica nanoparticles than were macrophages [113]. 

SWNTs (1.56-800 pg/ml) Human A 549 cells 1 day SWNTs have low acute toxicity against A549 cells Davoren et al. (2007)... 

Figure 11.2 Morphological differences between human alveolar epithelial cells in primary culture (A and C) and the A549 cell line (B and D). Cells are visualised by light microscopy (A and B) and immunofluorescence microscopy (C and D) using an antibody against a tight junctional protein, occludin.

Foster KA, Oster CG, Mayer MM, Avery ML, Audus KL (1998) Characterization of the A549 cell line as a type II pulmonary epithelial cell model for drug metabolism. Exp Cell Res 243(2) 359-366... 

Kobayashi S, Kondo S, Juni K (1995) Permeability of peptides and proteins in human cultured alveolar A549 cell monolayer. Pharm Res 12(8) 1115-1119... 

Wang Z, Zhang Q (2004) Transport of proteins and peptides across human cultured alveolar A549 cell monolayer. Int J Pharm 269(2) 451—456... 

Balis JU, Bumgarner SD, Paciga JE, Paterson JF, Shelley SA (1984) Synthesis of lung surfactant-associated glycoproteins by A549 cells description of an in vitro model for human type II cell dysfunction. Exp Lung Res 6(3-4) 197-213... 

In MDR cells, a significant fraction of P-gp is found associated with caveolin-rich membranes, and there is a substantial increase in the number of caveolae and caveolin-1 protein level. For example, both multidrug resistant human colon adenocarcinoma HT-29 cells and adriamycin-resistant breast adenocarcinoma MCF-7 cells display about a 12-fold increase in caveolin expression, which correlates with an approximate fivefold increase in morphologically identifiable caveolae [55], In addition, these cells exhibit increased amounts of phospholipase D and lipids such as cholesterol, glucosylceramide, and sphingomyelin [56, 57], Similarly, taxol-resistant A549 cells display both increased caveolin expression and caveolae numbers [58], While these correlations track with MDR, they do not suggest a simple mechanism for the role of... 

C. P. Yang, F. Galbiati, D. Volonte, S. B. Horwitz, and M. P. Lisanti. Upregulation of caveolin-1 and caveolae organelles in Taxol-resistant A549 cells. FEBS Lett. 439 368-372 (1998). 

Courage C, Snowden R, Gescher A (1996) Differential effects of staurosporine analogs on cell cycle, growth and viability in A549 cells. Br J Cancer 74 1199-1205... 

A549 cells (Human lung adenocarcinoma epithelial cell line). Subculture once a week, maintain in 5% CO, at 37°C. 

Seed A549 cells in 6-well plates with a seeding density of 1 x 10 cells per well (see Note 5). 

Fig. 4. Western blot image of GAPDFI expression in A549 cells at 72 h post transfection with LAFI4-L1/siRNA complexes. The intensity of GAPDFI protein band decreases as the amount of siRNA per well increases. For the negative control (-ve), 100 pmol siRNA/well was used. p-Actin was used as internal control.

ACEA A549 cell impedance measures over 7... 

A549 cells S phase arrest, (phosphorylation of Rb, (p21WAF1/ CIP1 (p53-dependent) Kim et al., 2003a... 

Kim YA, Lee WH, Choi TH, Rhee SH, Park KY, Choi YH. 2003a. Involvement of p21WAFl/CIPl, pRB, Bax and NF-kB in induction of growth arrest and apoptosis by resveratrol in human lung carcinoma A549 cells. Int J Oncol 23 1143-1149. 

Lee J, Jung HH, Im YH, Kim JH, Park JO, Kim K, Kim WS, Ahn JS, Jung CW, Park YS, Kang WK, Park K. 2006. Interferon-alpha resistance can be reversed by inhibition of IFN-alpha-induced COX-2 expression potentially via STATI activation in A549 cells. Oncol Rep 15 1541-1549. 

HoK induces cytosolic and nuclear delivery of ODN. A549 cells were incubated for four 314... 

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