A549 cell line

Figure 11.2 Morphological differences between human alveolar epithelial cells in primary culture (A and C) and the A549 cell line (B and D). Cells are visualised by light microscopy (A and B) and immunofluorescence microscopy (C and D) using an antibody against a tight junctional protein, occludin.

Foster KA, Oster CG, Mayer MM, Avery ML, Audus KL (1998) Characterization of the A549 cell line as a type II pulmonary epithelial cell model for drug metabolism. Exp Cell Res 243(2) 359-366... 

Croxtall, J.D., Choudhury, Q., and RJ. Flower, 1998, Inhibitory effect of peptides derived from the N-terminus of lipocortin 1 on arachidonic acid release and proliferation in die A549 cell line identification of E-Q-E-Y-V as a crucial component Br J Pharmacol. 123(5) 975—83. 

Cell lines of both airway and alveolar epithelial origin are available (immortal cells, transformed or carcinoma-derived), the most commonly used being the CALU-3 cell line derived from airway epithelium [37], and the A549 cell line derived from a bronchioalveolar carcinoma [38]. However, these cultured cell lines share only limited similarity in morphology, biochemical characteristics, and barrier properties (low epithelial resistance due to the lack of tight junctions) with the epithelia in vivo. Consequently, the results of absorption and metabolism studies based on cultured cell lines should be interpreted with caution. 

Fig. 3 Cytotoxic activity of compounds 12-14 against the A549 cell line...

Secobatzelline A and secobatzelline B were isolated from a deepwater marine sponge of the genus Batzella. Secobatzellines A and B inhibited the phosphatase activity of calcineurin, and secobatzelline A inhibited the peptidase activity of CPP32. Both compounds showed in vitro cytotoxicity against P388 and A549 cell lines [42]. 

Lung cancer Cytochrome P450 lAl (CYPlAl) and telomerase (hTERT) Rapid cell death A549 cell line 142... 

Type I insulin-like growth factor receptor (IGF-IR) Induces apoptosis inhibits tumor growth A549 cell line xenograft model 145... 

Very recently, from the marine-derived fungus Acremonium strict urn a new metabolite, acremoline 90, was isolated, which is a modified guanine base attached to an isoprene unit via a 1//-azirine moiety. This compound exhibited weak cytotoxicity against the A549 cell line (Figure 3.30) [72]. 

A9, A431 and A549 cell lines, 3478 12a,13a-Aziridinyl epothilone derivatives, 990 Aaptos ciliata, 1292 AATs. See Anthocyanin acyltransferases (AATs)... 

Influenza Flu NA, HA, RNA polymerase assays Plaque assay, HA, HAI, RT-PCR (MDCK, A549 cell lines)... 

Streptomyces sp. Antibacterial substance Bacillus subtilis. Staphylococcus aureus, Escherichia coli, Proteus vulgaris. Salmonella typhi, human lung carcinoma A549 cell line Kharat et al. (2009)... 

The same species had already led to terpeptins A and B, two indolic enamide diastereoisomers moderately cytotoxic against the A549 cell line (Lin et al, 2008). 

---