Zymomonas mobilis, ethanol production

Other continuous membrane fermentors show similar results. In the continuous production of ethanol from Zymomonas mobilis, the production rate was 120 g/hr/E compared to less than 8 g/hr/E for the batch system.77... 

Owing to diminishing fossil fuel reserves, alternative energy sources need to be renewable, sustainable, efficient, cost-effective, convenient and safe.1 In recent decades, microbial production of ethanol has been considered as an alternative fuel for the future because fossil fuels are depleting. Several microorganisms, including Clostridium sp. and yeast, the well-known ethanol producers Saccharomyces cerevisiae and Zymomonas mobilis, are suitable candidates to produce ethanol.2,3... 

Use of biofilm reactors for ethanol production has been investigated to improve the economics and performance of fermentation processes.8 Immobilisation of microbial cells for fermentation has been developed to eliminate inhibition caused by high concentrations of substrate and product, also to enhance productivity and yield of ethanol. Recent work on ethanol production in an immobilised cell reactor (ICR) showed that production of ethanol using Zymomonas mobilis was doubled.9 The immobilised recombinant Z. mobilis was also successfully used with high concentrations of sugar (12%-15%).10... 

Scott, C. D., Ethanol Production in a Fluidized-Bed Bioreactor Utilizing Flocculating Zymomonas Mobilis with Biomass Recycle, Biotechnol. Bioeng. Symp. Ser., 13 287 (1983)... 

Weuster-Botz, D., Continuous Ethanol Production by Zymomonas mobilis in a Fluidized Bed Reactor. Part I. Kinetic Studies of Immobilization in Macroporous Glass Beads, Appl. Microbiol. Biotechnol., 39 679 (1993)... 

Kim, C.H. and Rhee, S.K., Ethanol production from Jerusalem artichoke by inulinase and Zymomonas mobilis, Appl. Biochem. Biotechnol., 23, 171-180, 1990. 

Allias, J.-J., Favela-Torres, E., and Baratti, J., Continuous production of ethanol with Zymomonas mobilis growing on Jerusalem artichoke juice, Biotechnol. Bioeng., 29, 778-782, 1987. 

Toran-Diaz, I., Delezon, C., and Buratti, J., The kinetics of ethanol production by Zymomonas mobilis on fructose medium, Biotechnol. Lett., 5, 409 -12, 1983. 

Gold, R. S., Meagher, M. M., Tong, S., Hutkins, R. W., and Conway, T. 1996. Cloning and expression of the Zymomonas mobilis production of ethanol genes in Lactobacillus casei. Curr. Microbiol., 33,256-260. 

Nichols, N. N., Dien, B. S., and Bothast, R. J. 2003. Engineering lactic acid bacteria with pyruvate decarboxylase and alcohol dehydrogenase genes for ethanol production from Zymomonas mobilis. J. Ind. Microbiol. Biotechnol., 30, 315-321. 

Zhang, M., Eddy, C., Deanda, K., Franden, M. A., Finkelstein, M., and Picataggio, S., Metabolic engineering of zymomonas-mobilis for ethanol-production from renewable feedstocks. Abstracts of Papers of the American Chemical Society 1995, 209, 115-BTEC. 

Metabolic engineering [39, 40] has been used to impart the capacity for ethanol production and xylose fermentation in E. coli [41-45], Klebsiella oxytoca [A6,A7],Zymomonas mobilis [48,49] andS. cerevisiae [50-53]. In general, attempts at metabolic engineering have been more successful in bacteria than in yeasts. Although the reasons are not entirely clear, the smaller genomes and fewer feedback regulatory factors found in bacteria make these organisms much easier to work with. 

Escherichia coli Ethanol production Integration of pyruvate dearboxylase and alcohol dehydrogenase 11 from Zymomonas mobilis onto the chromosome of E. coli improve d the stability of the genes over the plasmid-based system, and the ethanol yield was near the maximum theoretical yield on 10% glucose and 8% xylose 48, 98... 

Altuntas E.G. and Ozcelik F. Ethanol production from starch by co-immobilized amyloglucosidase— Zymomonas mobilis cells in a continuously-stirred bioreactor. Biotechnology and Biotechnological Equipment 27 (1) (2013) 3506-3512. 

Another promising micro-organism for the production of fuel ethanol is Zymomonas mobilis. This facultative anaerobic. Gram-negative bacterium degrades glucose by the so-called Entner-Doudoroff-pathway with the consequence that only 1 mol of ATP is produced during the breakdown of... 

Similar optimization studies were experimentally verified for ethanol production with Zymomonas mobilis (e.g., Charley et al., 1983). A two-stage CSTR system with cell recycling in the second stage resulted in high final alcohol concentrations (>100 g/1) at quite high overall volumetric produc-... 

TABLE 7.1 Ethanol Production by Zymomonas mobilis and Saccharomyces carlsbergensis... 

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