Amyloglucosidase immobilization

The Lineweaver - Burk plot of the amyloglucosidase immobilized on cellulose carbonate is practically linear, while for the enzyme coupled on CICMC at different pH values (Figure 14, curves 2 and 3) the linearity is maintained only for increased concentrations of the substrate with increased value. In the former case the plot linearity attests that the amyloglucosidase molecules are located mainly... 

Fig. 14. Lineweaver - Burk plots for a-amylase at pH=5.8 (1) amylo-glucosidase immobilized on ClCMC, at pH=8 (2) and at pH = 9 (3) amyloglucosidase immobilized on cellulose carbonate at pH= 8 (4),...

Storey, K.B., Duncan, J.A., Chakrabarti, A.C. Immobilization of amyloglucosidase using two forms of polyurethane polymer, Appl-Biochem-Biotechnol. 1990 Mar 23... 

Huang, T.C. and Chen, D.H., Kinetic studies on urea hydrolysis by immobilized urease in a batch squeezer and flow reactor, Biotechnol. Bioeng., (1992) 40,10,1203-09. Storey, K.B., Duncan, J.A., and Chakrabarti, A., Immobilization of amyloglucosidase using two forms of polyurethane polymer, Appl. Biochem. Biotechnol., (1990) 23, 3, 221-36. 

In a second example, Storey et al. demonstrated that one could covalently immobilize amyloglucosidase using hydrophilic prepolymers. A 5-mg/ml solution of the enzyme was mixed with an equal volume of prepolymer. The method was judged superior as a support for enzyme immobilization. The percent activity inuno-bili/ed in the polyurethane foams was 25 1.5%. 

Preparation and comparison of catalytic activity of immobilized Amyloglucosidase with native enzyme on dextrin - hydrolysis... 

To determine the catalytic activity of chitin-immobilized Amyloglucosidase on the hydrolysis of dextrin, an experiment was conducted in which rate of hydrolysis of dextrin was monitored with the increase of time. To lOOmg of dextrin 2 units of chitin-immobilized... 

Altuntas E.G. and Ozcelik F. Ethanol production from starch by co-immobilized amyloglucosidase— Zymomonas mobilis cells in a continuously-stirred bioreactor. Biotechnology and Biotechnological Equipment 27 (1) (2013) 3506-3512. 

Fig.4. (top) Reaction scheme for the enzymatic determination of starch as executed in the FIA manifold shown below, where AMG refers to amyloglucosidase and GDH to glucose dehdrogenase, both of which enzymes are immobilized on porous glass in separate packed-bed reactors. The detection of the generated NADH is facilitated amperometrically by means of a modified electrode at 0 mV vs. Ag/AgCl, 0.1 M KCl. Flow rates are given in ml/min injected volume 30... 

Immobilization of Amyloglucosidase. The coupling of amyloglucosidase (E.C, 3 2.1.) on CICMC was performed at different enzyme/carrier ratios (Table 1). 

The activity of immobilized amyloglucosidase decreases with increasing pH (Figure 9) at room temperature, probably due to the destruction of the secondary structure, hence of the active centers ... 

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