Vegetable anthocyanin

Many solvents such as methanol, acetone, ethanol, and water are used for anthocyanin extraction due to their polar character since most anthocyanins occur naturally as glycosides. Anthocyanin glycosides have higher solubility in water than the corresponding aglycons. In addition, in most fruits and vegetables, anthocyanin pigments are located in cells near the surface. " ... 

DFR Forsythia Xintermedia Vegetative anthocyanin pigmentation increased 344... 

Anthocyanins, a class of flavonoids, are responsible for the red and purple colours associated with many fraits and vegetables. Anthocyanins occur in epidermal and subepidermal cells, dissolved in vacuoles or accumulated in vesicles called anthocyanoplasts. In general, the anthocyanin content in most fmits and vegetables varies between 0.1 and 1% of the diy matter content (Swain and Bate-Smith, 1962). 

Wu, X. and Prior, R.L., Identification and characterization of anthocyanins hy high performance liquid chromatography-electrospray ionization-tandem mass spectrometry in common foods in the United States vegetables, nuts, and grains, J. Agric. Food Chem., 53, 3101, 2005. 

Fruifs and vegetables also contain ofher bioactive substances such as polyphenols (including well-known pigments anthocyanins, flavonols) and non-provitamin A carotenoids (mainly lycopene, lutein, and zeaxanthin) that may have protective effects on chronic diseases. Polyphenols and carotenoids are known to display antioxidant activities, counteracting oxidative alterations in cells. Besides these antioxidant properties, these colored bioactive substances may exert other actions on cell signaling and gene expression. 

Betalains are water-soluble nitrogen-containing pigments responsible for the red and violet (betacyanins class) and yellow (betaxanthins class) colors found in many flowers, fruits, and occasionally in vegetative tissues of plants of most families of the Caryophyllales order (except the Caryophyllaceae and Molluginaceae families, which have anthocyanins instead)." ... 

The most widespread anthocyanidin in foods is cyanidin, as can be seen in Table 4.3.1 through Table 4.3.4. It was found in amounts greater than 10% of the total anthocyanin content in 39 of 44 different fruits listed in Table 4.3.1 and in 9 of 13 vegetables shown in Table 4.3.3. On the other hand, cyanidin was presented in high amounts only in non-Vitis vinifera species, such as cv. Concorde " and Cynthiana (Table 4.3.2). 

Petnnidin was detected in amounts above 10% of the total anthocyanin content in only 4 of the 44 frnits listed in Table 4.3.1, in 1 of 13 vegetables shown in Table 4.3.3, in 2 kinds of beans and in 1 nut (Table 4.3.4). Petunidin was found in different grape species snch as cv. Cabernet Sauvignon, ° variety Isabelle, " cv. Pallagrello, cv. Piedirosso Vesnvio, " cv. Nerello Mascalese, and cv. Clinton." None of the foods listed in the fonr tables presented petnnidin as the sole aglycone. 

Anthocyanins from apples, cranberries, black chokeberries, - blackberries of an unknown cultivar, - highbush blueberries, - - Vaccinium padifolium blueberries, cv. Balaton black cherries, and black lentils" contained arabi-nose. This pentose was not found in any of the vegetables presented in Table 4.3.3. Even less widespread is xylose, found in Red Delicious apple cultivars, black chokeberries, different cultivars of blackberries, evergreen blackberries, red currants, Vaccinium padifolium blueberries, different cultivars of red rasp-berry, - - and black carrots. " ... 

Sambubiose was less widespread, as a constituent of the major anthocyanins from red currants," elderberries," Davidson s plums, black raspberries, and small red beans, - and hnked to minor anthocyanins from agai " and Vaccinium padifolium blueberries. - Considering the anthocyanins identihed at least with MS, as far as we are concerned, this disaccharide was not reported in vegetables, cereals, or grains. 

Quantitative and qualitative anthocyanin composition must be known in order to determine the feasibility of application of new plant materials as anthocyanin-based colorant sources and to understand the relationships of structures and functions of anthocyanins. In addition, anthocyanin compositions of fruits and vegetables have also been used to detect adulteration of anthocyanin-based products - and as indicators of product quality. - ... 

Mazza, G. and Miniati, E., Anthocyanins in Fruits, Vegetables, and Grains, CRC Press, Boca Raton, EL, 1993. 

Thousands of polyphenols from fruits (grapes, apples, etc.), vegetables (horse beans), and teas have been identified, many having good coloring properties, especially anthocyanins and some flavonoids. Well-documented reviews discuss the coloring capacities of some polyphenols including procyanidins. - Detailed presentations of anthocyanin and flavonoid properties and analysis are included in Sections 2.3, 4.3, and 6.3. The soluble proanthocyanidins of the colored horse bean Viciafaba L. seed coats were isolated and separated by solvent partition. 

Plants including fruits and vegetables are a vast reservoir of different phytochemicals. As stated previously, flavonoids are a diverse group of polyphenolic compounds, some of which are relatively stable, whereas others such as anthocyanins are labile under ambient conditions. Sample preparation is of paramount importance in studying flavonoids because a good method prevents compounds of interest from being degraded... 

Sanchez-Morcno (2002) considered that this assay is an easy and accurate method for determining antioxidant capacity in fruit and vegetable samples. The DPPH assay has been used to determine the antioxidant activity of polyphenols (Sanchez-Moreno and others 1998 Bao and others 2004) flavonols (Jimenez and others 1998 1999 Choi and others 2002) anthocyanin-based natural colorants from berries (Espin and others... 

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