Uronic acids carbohydrates containing

Chai, W., Rosankiewicz, J. R., and Lawson, A. M. (1995). TLC-LSIM of neoglycolipids of glycosaminoglycan disaccharides and of oxymercuration cleavage products of heparin fragments that contain unsaturated uronic acid. Carbohydr. Res. 269 111-124. 

De Ruiter, G. A., Schols, H. A., Voragen, A. G. J., and Rombouts, F. M., Carbohydrate analysis of water-soluble uronic acid-containing polysaccharides with high-performance anion-exchange chromatography using methanolysis combined with TFA hydrolysis is superior to four other methods, Anal. Biochem., 207, 176, 1992. 

Chaplin used methanolysis for the analysis of carbohydrates in glycoproteins. His method was a variation of the foregoing procedures, with an improvement of using tert-hvAyX alcohol to remove hydrogen chloride by coevaporation, instead of prolonged trituration with silver carbonate. His method is useful for samples containing uronic acids and lipids. Mononen studied methanolysis, followed by deamination and reduction with borohydride, for determination of the monosaccharide constituents of glycoconjugates. This method was applied to a lipid-free, protein fraction of rat brain. 

Carbohydrate/O-acetyl content of P. deltoides LCCs appears in Table III. All purified LCCs were 0-acetylated moieties containing varying amounts of glucose, mannose, xylose, and uronic acids. Relative to xylose, the highest levels of uronic acid and 0-acetyl content were encountered in LCCs 1 and 2. LCC 1 was the sole moiety to contain galactose. Xylose was the most abundant sugar found in both LCCs 3 and 4, both of which also contained nearly equimolar amounts of glucose and mannose. 

All fractions should have their combined neutral sugars and uronic acid (and cellulose if appropriate) contents checked against the weight of the fraction. Except for the initial fractions, which contain protein and other cellular components, the combined carbohydrate analyses figures should agree approximately with the weight of the fraction. 

Traces of the complex carbohydrates contained in the juice survive the processing and appear in the molasses analytical data indicate that they probably consist largely of pectins and pentosans.10 Quantitative estimations based on the ash-free solids of Louisiana molasses revealed the presence of 2% uronic acids and 0.5% methoxyl.16 Heat-modified... 

J. Kiss, /S-Eliminativc degradation of carbohydrates containing uronic acid residues, Adv. Carbohydr. Chem., 29 (1974) 229-303. 

In 1933, Fischer and Schmitz first claimed to have isolated a pure heparin preparation. Their lengthy extraction and isolation procedure culminated in the isolation of a microcrystalline brucine salt 32 times as active as the starting material. No evidence other than that of appearance was quoted in support of the claimed crystallinity of the product. It was concluded that heparin was a carbohydrate (Molisch test) and contained a uronic acid. 

These proteins are composed of simple proteins in combination with carbohydrates like mucopolysaccharides, which include hyaluronic acid and chondroitin sulphates. On hydrolysis, mucopolysaccharides yield more than 4% of amino-sugars, hexosamine and uronic acid e.g., ovomucoid from egg white. Soluble mucoproteins are neither readily denatured by heat nor easily precipitated by common protein precipitants like trichloroacetic acid or picric acid. The term glycoproteins is restricted to those proteins that contain small amounts of carbohydrate usually less than 4% hexosamine. 

Polyuronides may be defined as polysaccharides that contain one or more uronic acid units in their molecular structures. They have a frequent and wide occurrence in nature. Much of the carbohydrate material in plants belongs to the group. It includes all pectic materials and plant gums and many plant mucilages, hemicelluloses and gel-forming substances and some microbial polysaccharides. These substances are to be looked for in water-soluble plant exudates and mucilages, as well as in water and alkaline extracts of most plant materials. This review will be limited to the polyuronides occurring in plants. 

Examination of the chemical properties of the active polysaccharide F-8 and F-9 shows that F-8 contained 89.3% carbohydrate as glucose and 7.2% uronic acid as galacturonic acid, while F-9 contained 95.5% carbohydrate and 7.5% uronic acid. These polysaccharide do not contain... 

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