Toxins immunization

Toxin Immune mediators Synergy with LPS or TNF Apoptosis DNA- fragmentation References... 

Breinig, F., Sendzik, T, Eisfeld K., Schmitt, M. J. Dissecting toxin immunity in virus-infected killer yeast uncovers an intrinsic strategy of self-protection. Proceedings of the National Academy of Sciences 2006,103(10), 3810-3815. 

Dmg receptors are chemical entities which are typically, but not exclusively, small molecules that interact with cellular components, frequently at the plasma membrane level (1,2). There are many types of receptors heat, light, immune, hormone, ion channel, toxin, and vims are but a few that can excite a cell. The receptor concept can be appHed generally to signal recognition processes where a chemical or physical signal is recognized. This recognition is translated into response (Fig. 3) and the process can be seen as a flow of information. 

Human immunoglobulin preparations from pools of a great number of people (>1,000) with assumed antibodies against common viruses are used as a means of passive immunization in acute infections. More specific antibody preparations with high titers from patients who recovered recently from a viral disease or were immunized against toxins are also available in... 

Both vaccines and toxoids are administered to stimulate the immune response within the body to specific antigens or toxins. These agents must be administered before exposure to the pathogenic organism. The initiation of the immune response, in turn, produces resistance to a specific infectious disease. The immunity produced in this manner is active immunity. Display 54-5 gives examples of indications for use of toxoids and vaccines. 

Figure 9. Anti-PbTx antiserum inhibition of [ H]PbTx-3 binding to its receptor site in rat brain membrane preparations. Labeled toxin (0.5 nM in 1 ml PBS) was incubated with rat brain membranes (125 fig total protein) and increasing amounts of anti-PbTx antiserum (- -) or pre-immune serum (- -) for 1 hr at 4 C. Membrane-bound radioactivity was then measured in a centrifugation assay as previously described (8),...

Kentolysin Compared to Heliantholysin. Stoichactis helianthus occurs in the Caribbean region whereas another species, Stoichactis kenti is distributed in the Indo-Pacific area. The latter produces a toxin, kentolysin, that is similar to, but not identical with heliantholysin (6). The amino acid compositions of the two polypeptides show a distinct resemblance but appear to differ significantly in number of residues of lysine, methionine, tyrosine and histidine. IgG from a rabbit immunized against heliantholysin neutralizes both heliantholysin and kentolysin, but neutralization of the homologous toxin is more efficient (Table III). It can be seen that in the concentrations used, the IgG failed to neutralize the related lytic peptides of Condylactis gigantea and Epiactis prolifera. 

Ahn SY, Cho CH, Park KG, Lee HI, Lee S, Park SK, Lee IK, Koh GY (2004) Tumor necrosis factor-alpha induces fractaUdne expression preferentially in arterial endothelial cells and mithramycin A suppresses TNF-alpha-induced fractaUdne expression. Am J Pathol 164 1663-1672 Alfano M, Schmidtmayerova H, Amelia CA, Pushkarsky T, Bukrinsky M (1999) The B-oligomer of pertussis toxin deactivates CC chemokine receptor 5 and blocks entry of M-tropic HIV-1 strains, [see comments]. J Exp Med 190 597-605 Ambrosini E, Alois F (2004) Chemokines and glial cells a complex network in the central nervous system. [Review] [239 refs]. Neurochem Res 29 1017-1038 Azuma Y, Ohura K (2002) Endomorphins 1 and 2 inhibit IL-10 and IL-12 production and innate immune functions, and potentiate NE-kappaB DNA binding in THP-1 differentiated to macrophagelike cells. Scand J Immunol 56 260-269... 

Vaccines achieve their protective effects by stimulating a recipient s immune system to synthesize antibodies that promote the destruction of infecting microbes or neutralize bacterial toxins. This form of protection, known as active immunity, develops in the course of days and in the cases of many vaccines develops adequately only after two or three doses of vaccine have been given at intervals of days or weeks. Once established. 

Since these vaecines are imable to evoke a natural infection profile with respeet to the release of antigen they must be administered on a number of occasions. Immunity is not complete until the course of immunization is complete and, with the exeeption of toxin-dominated diseases (diphtheria, tetanus) where the immimogen is a toxoid, will never match the performance of live vaccine delivery. Specificity of the immrme resporrse generated in the patient is initially low. This is particularly the case when the vaeeine is composed of a relatively crude cocktail of killed cells where the immime response is direeted only partly towards antigenic components of the cells that are assoeiated with the infeetion process. This increases the possibility of adverse reaetions in the patient. 

This is an acute, non-invasive infectious disease associated with the upper respiratory tract (Chapter 4). The incubation period is fiom 2 to 5 days although the disease remains communicable for up to 4 weeks. A low molecular weight toxin is produced which affects myocardium, nervous and adrenal tissues. Death results in 3-5% of infected children. Diphtheria immunization protects by stimulating the production of an antitoxin. This antitoxin will protect against the disease but not against infection of the respiratory... 

Cholera toxin and related toxins act as immune modulators, with potential use as adjuvants and as therapeutic agents in the treatment of immunologically mediated human disease. 

Health benefits — Research reports indicate that natural (3-carotene possesses numerous benefits for the human body and consistently supports the use of (3-carotene as part of the human diet. The human body converts (3-carotene to vitamin A via body tissues as opposed to the liver, hence avoiding a build-up of toxins in the liver. Vitamin A is essential for the human body in that it assists the immune system and helps battle eye diseases such as cataracts and night blindness, various skin ailments such as acne, signs of aging, and various forms of cancer. 

Microbial risks are mostly due to single exposures (except for microbial toxins) chemical risks are affected by chronic duration of exposure. Responses to infective pathogens are probably more variable as compared to chemical agents due to different subpopulations and depending on immune status. 

It has been postulated that Chlamydia may produce a heat shock protein that causes tissue damage through a delayed hypersensitivity reaction. C. trachomatis may also possess DNA evidence of toxin-like genes that code for high-molecular-weight proteins with structures similar to Clostridium difficile cytotoxins, enabling inhibition of immune activation. This may explain the observation of a chronic C. trachomatis infection in subclinical PID. 

In the early 1900s, a balanced mixture of diphtheria toxin and antitoxin was found to produce active immunity in both animals and humans. This preparation gained widespread acceptance and protected approximately 85% of recipients. Several years later, diphtheria toxoid was developed by treating the toxin with small amounts of formalin. This process caused the toxin to lose its toxic properties while maintaining its immunogenic properties. In the mid-1920s, the addition of an alum precipitate enhanced the immunogenic properties of the toxoid. 

Kaminski NE, Koh WS, Yang KH, Lee M, Kessler FK. Suppression of the humoral immune response by cannabinoids is partially mediated through inhibition of adenylate cyclase by a pertussis toxin-sensitive G-protein-coupled mechanism. Biochem Pharmacol 1994 48 1899-1908. 

Bacteria, viruses, and rickettsiae have similar symptom progressions in that exposure is followed by a period of reproductive growth (often nonsympto-matic) in the body. As their numbers increase, they often eventually overcome the immune system. Many produce toxins that interfere with bodily functions. Purified toxins such as botulinum toxin (produced by the Clostridium botulinum bacteria) act in a similar manner to chemical agents since, as complex chemical compounds, they do not reproduce but immediately interfere with bodily functions. However, most toxins are not absorbed through the skin, as... 

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