Sodium EDTA extractant

The mash from the Streptomyces aureofaciens fermentation broth is acidified and filtered. The filtrate is adjusted to the desired pH, usually 7-8.5, and various flocculating or chelating agents may be added (e.g., vinyl acetate-maleic anhydride copolymer, sodium EDTA, ammonium oxalate, Arquad). The precipitate is (1) stirred with filter aid, filtered, stirred with HC1, refiltered, mixed with 2-ethoxyethanol, filtered, washed, and the filtrates are combined, acidified with HC1, NaCl is added, and the crystals are collected, washed with 2-ethoxyethanol, water, and ethanol, and dried (67), or (2) extracted into methyl isobutyl ketone, the extracts are combined, filtered, and acidified with HC1, and the crystals are collected and washed with water, 2-ethoxyethanol, and isopropanol, and vacuum-dried. If the crystals are greenish, they are treated with sodium hydrosulfite at pH 1.8, filtered, washed, and dried as in (1) above (68). 

Bacterial Extract Protease CocktaiL Inhibitors present are pepstatin A, 4-(2-amino-ethyl)benzenesulfonyl fluoride (AEBSF), rrans-epoxysuccinyl-L-leucylam-ido(4-guanidino)butane (E-64), bestatin, and sodium EDTA. 

Fig. 4.2. Isolation of environmental DNA from soils and sediments by the direct lysis approach. The DNA isolation of soil and sediment samples is based on the direct lysis method of Zhou et al. [16]. 50 g of each environmental sample are mixed with 135 ml of DNA extraction buffer (100 mM Tris/HCI, pH 8.0,100 mM sodium EDTA, 100 mM sodium phosphate, 1.5 M NaCI, 1 % (w/v) CTAB) and 1 ml of proteinase K (10 mg/ml) in GS3 tubes by horizontal shaking for 30 min at 37°C. Subsequently 15 ml of 20 % (w/v), SDS is added and the samples are incubated in a 65 °C water bath for 2 h with gentle end-over-end inversion every 15 to 20 min. After centrifugation at 6000 x g for 10 min at room temperature the resulting supernatants are transferred into new GS3 tubes. The remaining pellets are extracted two more times by suspending them in 45 ml of extraction...

Iron-bound phosphate Calcium-bound phosphate Acid-soluble organic phosphate Sodium hydroxide-extractable phosphorus Fe(OOH) P CaCOj P ASOP NaOH3,-P 0.02 M Ca-NTA/dithionite, pH 7.8-8.0 0.05 M Na-EDTA, pH about 8.0 0.5 M HCI or 0.25 M HjSO (30 min) 2.0 M NaOH (OO C, 30 min)... 

Pressure fluid extraction (PFE) PFE mainly consists of a static or dynamic pressure and temperature assisted liquid-solid extraction. Phenyl ureas from soils and sulfonyl ureas from maize samples were extracted under pressure with methanol at 50°C, using a dynamic setup at 1 ml min and using a total extractant volume of 25 ml. 2,4-D, 2,4,5-T, dicamba, trichlorpyr, and bentazone have been in situ derivati-zed during the PFE procedure. The variables temperature, pressure, static extraction time, and derivatization reagent amount should be subjected to optimization in order to increase recoveries. Addition of sodium EDTA in the extraction chamber strongly increases 2,4-D recovery. 

Figure 15. NMR spectra from fresh, intact frog gastrocnemius muscle, a perchloric acid extract of fresh frog muscle mince adjusted to pH 7 for P analysis (Na" counter cation), and the above perchloric acid extract to which sodium EDTA had been added (0.2 m, pH 7). From Barany et al (1975).

Commercial Hquid sodium alumiaates are normally analyzed for total alumiaa and for sodium oxide by titration with ethylene diaminetetraacetic acid [60-00-4] (EDTA) or hydrochloric acid. Further analysis iacludes the determiaation of soluble alumiaa, soluble siHca, total iasoluble material, sodium oxide content, and carbon dioxide. Aluminum and sodium can also be determiaed by emission spectroscopy. The total iasoluble material is determiaed by weighing the ignited residue after extraction of the soluble material with sodium hydroxide. The sodium oxide content is determiaed ia a flame photometer by comparison to proper standards. Carbon dioxide is usually determiaed by the amount evolved, as ia the Underwood method. 

Step 2. The pellets are extracted with 10 mM sodium phosphate buffer, pH 6.7, containing 2mM EDTA and 0.2 M NaCl, and chromatographed on a gel-filtration column (Ultragel AcA 54 LKB) using the same pH 6.7 buffer. The photoprotein is eluted slightly before a brownish substance. 

High-performance liquid chromatography (HPLC) with a micellar mobile phase or with a selective pre-column or reaction detection system has also been used to determine alkylenebis(dithiocarbamaes). ° Zineb and mancozeb residues in feed were determined by ion-pair HPLC with ultraviolet (UV) detection at 272 nm. These compounds were converted to water-soluble sodium salts with ethylenediaminetetra-acetic acid (EDTA) and sodium hydroxide. The extracts were ion-pair methylated with tetrabuthylammonium hydrogensulfate (ion-pair reagent) in a chloroform-hexane solvent mixture at pH 6.5-8.S. The use of an electrochemical detector has also been reported. ... 

Wakabayashi et al. [51] determined penicillamine in serum by HPLC. Serum (0.1 mL) was vortex-mixed for 30 s with 50 pL of 0.1% EDTA and 0.2 mL of 10% TCA. The solution was centrifuged at 1500 x g and filtered. A 5 pL portion was analyzed on a Shodex C18 column (15 cm x 4.6 mm i.d.), using a mobile phase of 19 1 methanolic 0.05 M phosphate buffer (pH 2.8) containing 1 mM sodium octylsulfate and 10 pM EDTA. Liver or kidney samples were similarly extracted, and the extracts were cleaned up on a Bond-Elut cartridge prior to HPLC analysis. Detection was effected with an Eicom WE-3G graphite electrode maintained at +0.9 V versus Ag/AgCl. The calibration graph was linear up to 500 ng, and the detection limits were 20 pg. For 1 pg of penicillamine added to serum, liver, or kidney, the respective relative standard deviations (n = 5) were 3.6, 5.1, and 4.4%. 

After 2 h incubation of the prepared antibody beads with UV-crosslinked extract in a cold room, the beads are washed 4 x with 100 /A RIPA buffer (50 mMTris-HCl pH 7.5, 150 rnMNaCl, 1% NP-40, 0.5% sodium deoxycholate, and 0.1% SDS) and lx with genomic DNA lysis buffer (50 mM Tris, pH 7.4, 10 mM EDTA, 500 mM NaCl, 2.5 mM DTT, 0.5 mM spermidine, 1% Triton X-100). Approximately 300 /(I of PK solution (1 mg/ml proteinase K in genomic DNA lysis buffer and 0.2 U//A RNase inhibitor) is added to the total lysate previously kept on ice and the beads are then incubated at 37° for 30 min. Gently flick the tubes to resuspend the beads every 10 min during the incubation. After removal of the proteinase K solution, 300 /A of RNA extraction solution (4 M guanidine thiocyanate, 0.5% sarkosyl, and 25 mM sodium citrate, pH7) is added to the beads, incubated for 10 min and the supernatant is mixed with 30 fig yeast tRNA (as a carrier) and 30 fil of 3 M sodium acetate. The RNA solution is phenol-chloroform extracted, ethanol-precipitated, and the pellet washed once with 70% ethanol. The dry pellet is used for 1st strand cDNA synthesis, followed by PCR analysis. The removal of proteins... 

In similar work, Sturgeon et al. [125] compared direct furnace methods with extraction methods for cadmium in coastal seawater samples. They could measure cadmium down to 0.1 pg/1. They used 10 pg/1 ascorbic acid as a matrix modifier. Various organic matrix modifiers were studied by Guevremont [116] for this analysis. He found citric acid to be somewhat preferable to EDTA, aspartic acid, lactic acid, and histidine. The method of standard additions was required. The standard deviation was better than 0.01 pg/1 in a seawater sample containing 0.07 pg/1. Generally, he charred at 300 °C and atomised at 1500 °C. This method required compromise between char and atomisation temperatures, sensitivity, heating rates, and so on, but the analytical results seemed precise and accurate. Nitrate added as sodium nitrate delayed the cadmium peak and suppressed the cadmium signal. 

In another spectrophotometric procedure Motomizu [224] adds to the sample (2 litres) 40% (w/v) sodium citrate dihydrate solution (10 ml) and a 0.2% solution of 2-ethylamino-5-nitrosophenol in 0.01 M hydrochloric acid (20 ml). After 30 min, add 10% aqueous EDTA (10 ml) and 1,2-dichloroethane (20 ml), mechanically shake the mixture for 10 minutes, separate the organic phase and wash it successively with hydrochloric acid (1 2) (3 x 5 ml), potassium hydroxide (5 ml), and hydrochloric acid (1 2) (5 ml). Filter, and measure the extinction at 462 nm in a 50 mm cell. Determine the reagent blank by adding EDTA solution before the citrate solution. The sample is either set aside for about 1 day before analysis (the organic extract should then be centrifuged), or preferably it is passed through a 0.45 xm membrane-filter. The optimum pH range for samples is 5.5 - 7.5. From 0.07 to 0.12 p,g/l of cobalt was determined there is no interference from species commonly present in seawater. 

Fig. 2. Sequential extraction of Arsenic (MG-magnesium chloride, PHOS-sodium hypo phosphate, HCL-hydrocihoric acid, OX-oxalic acid, ToCEB- titanium chloride with EDTA, NIT- nitric acid).

In a similar procedure [32] the sediment is wet oxidised with dilute sulphuric acid and nitric acids in an apparatus in which the vapour from the digestion is condensed into a reservoir from which it can be collected or returned to the digestion flask as required. The combined oxidised residue and condensate are diluted until the acid concentration is IN and nitrate is removed by addition of hydroxylammonium chloride with boiling. Fat is removed from the cooled solution with carbon tetrachlodithizone in carbon tetrachloride. The extract is shaken with 0.1M hydrochloric acid and sodium nitrite solution and, after treatment of the separated aqueous layer with hydroxylammonium chloride a solution of urea and then EDTA solution are added to prevent subsequent extraction of copper. The liquid is then extracted with a 0.01% solution of dithizone in carbon tetrachloride and mercury estimated in the extract spectrophotometrically at 485nm. 

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