Cold rooms

Crystd from methanol by adding dry ether to a ratio of 1 1 and cooled at 0°. Filter off the crystals in a cold room, wash with methanol/ether (1 2), then dry in a vacuum. [Hunter and Ludwig J Am Chem Soc S4 3491 1962.] The, free base has b 90-91 °/765mm, d 0.867, n 1.403 [Hunter and Ludwig Methods Enzymol 25 585 1 973.]... 

Air curtains for cooled rooms are used in all types of rooms with artificial cooling of air vegetable stores, cold rooms, freezers, air-conditioned plants and storehouses, etc. Installation of air curtains for cooled rooms considerably reduces cold losses through the open gate and also reduces undesirable variations in temperature in the gate zone inside and outside the cooled room. 

The solvent was evaporated off under reduced pressure, and the residual gum refluxed with concentrated hydrochloric acid (50 g) for 6 hours. The solution was aliowed to cool overnight. It was filtered from the phthalic acid crystals, and freeze-dried, and to the pink residue was added acetone (160 g) and ethyl acetate (50 g). The mixture was left in the cold room overnight and the clear pink supernatant liquid poured off. The pink gummy hydrochloride remaining in the flask was dissolved in water (20 g), saturated sodium acetate solution added until precipitation was complete, and the product collected and dried in a desiccator. The crude p-bis-(2-chloroethyl)-aminophenylalanine (3.6 g) was crystallized from methanol giving colorless needles, MP 172° to 174°C (decomp.) of p-bis-(2-chloroethyl)-aminophenylalanine. 

The total yield of the crude precipitation obtained in the above manner comprising about 1 kg is then dissolved In chloroform so as to form a 15% solution of a crude penicillin salt. To the filtered chloroform solution is added ethyl acetate slowly and with agitation until the solution becomes turbid as crystallization begins. Thereafter crystallization is allowed to proceed undisturbed for about 30-60 minutes in a cold room having a temperature of about 5°C. Sufficient ethyl acetate is slowly added to provide a final concentration of about 50% ethyl... 

Example 15.1 What will be the internal dimensions of a cold room to store 900 t of boxed frozen meat if the box size is 700 X 450 X 150 mm and the net weight 30 kg ... 

This is for cold rooms up to 100 m with normal service. For heavy service, i.e. a great deal of traffic through the doors, this figure can he increased hy 20-35%. 

A 3-1., three-necked flask fitted with a mechanical stirrer, a dropping funnel, and a thermometer is then charged with an aqueous solution of 2.2 moles of calcium hypochlorite [Hypochlorous acid, calcium salt] (Note 3), and the piperidine acetate prepared above is placed in the dropping funnel. The hypochlorite solution is stirred and cooled to 0° to — 5° with a methanol-ice bath, and the piperidine acetate is added dropwise over a period of 1.25 hours while the temperature is maintained below 0°. After a further 15 minutes of stirring, equal portions of the mixture are placed in two 2-1. separatory funnels and extracted three times with a total of about 1300 ml. of ether. The ether extract is placed in a 2-1. flask and dried over anhydrous sodium sulfate in a cold room at 4° overnight. After filtration to remove inorganic material, the bulk of the ether is removed by boiling on a water bath maintained below 60° (Note 4). 

In addition, the nurse determines if any controllable factors (eg, uncomfortable position, cold room, drafts, bright lights, noise, thirst) may be decreasing the patient s tolerance to pain. If these factors are present, the nurse corrects them as soon as possible. However, Hie nurse should not deny pain drugs or make the patient wait for the drug. Pain medication is delivered in a timely manner. 

The Pyrex tube was suspended, with capillary down, in a small-holed rubber stopper which, in turn, was fastened to a goniometer head by a length of stout copper wire. The solid material within the capillary was photographed in a cold room (4°C.) using copper x-radiation, a camera with radius 5 cm., and oscillation range 30°. The effective camera radius was established by superimposing a powder spectrum of NaCl during an exposure of the sample the lattice constant for NaCl at 4°C. was taken to be 5.634 A. 

Most of the reference substances/preparations are stored in cold rooms controlled at between 2°C and 8°C. However, a number of substances/preparations which are relatively unstable are stored at -20°C or, in a few cases, e.g. vaccines, at -8o°C. The reference substances/preparations may be stored for years under these conditions and their fitness for use is continually monitored as described above. Thus, the status of each reference substance/preparation is indicated in the catalogue. It is recommended that purchasers only order a sufficient amount for immediate use since the stability of the contents of opened vials or ampoules cannot be guaranteed. 

After 2 h incubation of the prepared antibody beads with UV-crosslinked extract in a cold room, the beads are washed 4 x with 100 /A RIPA buffer (50 mMTris-HCl pH 7.5, 150 rnMNaCl, 1% NP-40, 0.5% sodium deoxycholate, and 0.1% SDS) and lx with genomic DNA lysis buffer (50 mM Tris, pH 7.4, 10 mM EDTA, 500 mM NaCl, 2.5 mM DTT, 0.5 mM spermidine, 1% Triton X-100). Approximately 300 /(I of PK solution (1 mg/ml proteinase K in genomic DNA lysis buffer and 0.2 U//A RNase inhibitor) is added to the total lysate previously kept on ice and the beads are then incubated at 37° for 30 min. Gently flick the tubes to resuspend the beads every 10 min during the incubation. After removal of the proteinase K solution, 300 /A of RNA extraction solution (4 M guanidine thiocyanate, 0.5% sarkosyl, and 25 mM sodium citrate, pH7) is added to the beads, incubated for 10 min and the supernatant is mixed with 30 fig yeast tRNA (as a carrier) and 30 fil of 3 M sodium acetate. The RNA solution is phenol-chloroform extracted, ethanol-precipitated, and the pellet washed once with 70% ethanol. The dry pellet is used for 1st strand cDNA synthesis, followed by PCR analysis. The removal of proteins... 

Although several types of fluorescent beads were proposed as a microscopic fluorescence standard 30 years ago,2 beads have not been used as a proteinembedding matrix for routine IHC on FFPE tissue. We recently tested primary coated beads ( Dynabeads, Dynal, New York) that are coated with a goat anti-mouse antibody on the surface of the beads. In the first experiment, a monoclonal antibody to cytokeratin 7 (DAKO, 50pL/34.5pg) was bound to the beads by incubating with the beads (150 pL at a concentration of 109 beads/1 pL) at 4°C in a cold room with an automatic shaker for overnight. Incubation was followed by three phosphate-buffered saline (PBS) washes,... 

The desired amount of this collagen solution was diluted (0.2 ml to 10 ml) with citrate buffer, pH 4.0 and then dialyzed overnight against the buffer to be used. 2 ml of that solution were then diluted to 10 ml with the appropriate buffer, centrifuged in the cold (40000 g for one hour) and the supernatant immediately decanted into the viscometer in the cold room (10°C)... 

Surface samples were collected in snow pits under ultra-clean conditions described elsewhere [13] with the exception that samples for anion analysis were collected in polystyrene cups precleaned without the use of acids. Ice core samples were cleaned to remove surface contamination using the "dry-core" procedure involving rinsing and melting of exterior surfaces with ultra-pure water [13]. Shallow-depth firn cores are permeable and the dry-core rinsing is unsuitable. Therefore an inner core of 2.5 cm diameter was taken from intervals of the 7.6 cm diameter South Pole firn core using a specially-built precleaned stainless steel corer within a -15 °C cold room. Prior to this coring, exposed ends of core sections were shaved away with precleaned stainless steel chisels. 

Fig. 2.5. Granulating and sieving installation to produce a granulate of specified dimensions from frozen plates or large lumps. The mills can be seen in the background on top, the sieves can be seen in the lower part in front. The photograph was taken before installation in the cold room (photograph Vibra Maschinenfabrik Schultheis GmbH Co, D-6050 Offenbach am Main).

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