Mixing vortex

Mixing performance, 306 Blending, 324 Emulsions, 324 Extraction, 324 Gas-liquid contacting, 324 Gas-liquid dispersion, 325 Liquid-liquid dispersion, 325, 326 Mixing vortex, 311 Motionless mixing, see static mixing National Fire Protection Association, 399 Net positive suction head, 160-194 Available from system, 160, 188, 189,... 

Mix Vortex if possible.Vortexing is gentle, simple to perform and eliminates cross contamination Z620 Vortex Station... 

Samples of 50 pi plasma, standard or control plasma, 20 pi internal standard and 10 pi dithiothreitol solution are thoroughly mixed (vortex) in 1-ml Eppendorf tubes. The tubes are left to stand at room temperature for 15 min. Samples are then depro-teinised by the addition of 500 pi deproteinising acetonitrile solution, with thorough vortex mixing followed by centrifugation at 14,000 rpm (11,000 x g) for 5 min (4°C). Tandem mass spectrometry analysis is performed on 200 pi of the supernatant reserved in appropriate vials. If the analysis is not performed immediately, samples can be stored at -20°C until analysis. 

Cool to room temperature, remove, and discard DTT solution. Add iodoacetamide solution (100 pL) and incubate with occasional mixing (vortex) for 1 h in the dark at room temperature. 

Add 0.5 mL H20 followed by 0.6 mL N-butanol/petroleum ether (bp 40-60°C)/ ethyl formate (20/4/1 v/v) to the dried deacylated lipids. Vortex and centrifuge (lOOOg, 5 min). Carefully remove the upper organic phase and discard. Wash the lower, water soluble phase with a further 0.6 mL of N-butanol/petroleum ether (bp 40-60°C)/ethyl formate mix. Vortex, centrifuge and discard upper phase as above. Dry lower phase in vacuo. 

Mix 1 mL of solution 3 with 1 mL of solution 1 by vortex agitation. Then mix (vortex for 15 s) the resulting water-in-chloroform emulsion with a similar mixture composed of solution 2 and solution 4 (2.5 ml). 

Fill., filtration Evap., evaporation FI. det., fluorimetric detection SLE, solid-liquid extraction SEC, size-exclusion chromatography MD, microwave digestion LLE, liquid-liquid extraction Imm., immunoassay Phot, det., photometric detection FI. det., fluorimetric detection BOD, biological oxygen demand DOM, dissolved oxygen measurement SPE, solid-phase extraction Centr., centrifugation Mix., vortex mixing PCBs, polychlorinated biphenyls Pot. det., potentiometric detection TCP, 3,5,6-trichloro-2-pyridinol. 

Decant the medium and suspend the pellet in 25 mL buffer A prepared in step 3. Mix (vortex) the culture and re-centrifuge. 

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