Small-scale analysis

B. L. Schulz, N. H. Packer, and N. G. Karlsson, Small-scale analysis of O-linked oligosaccharides from glycoproteins and mucins separated by gel electrophoresis, Anal. Chem., 74 (2002) 6088-6097. 

Figure 5 shows an example of a well-functioning CV and a deteriorating CVC assessed in this way in several one-month intervals. In a small-scale analysis of... 

Levander, F., Svensson, M., Radstrom, P. Small-scale analysis of exopolysaccharides from Streptococcus thermophilus grown in a semi-defined medium. BMC Microbiol 2001, 1,23-27. 

Takemori, N. Komori, N. Matsumoto, H. Highly sensitive multistage mass spectrometry enables small-scale analysis of protein glycosylation from two-dimensional polyacrylamide gels. Electrophoresis 2006, 27, 1394-1406. 

First, considerably greater emphasis has been placed on semimicro techniques and their application to preparations, separations, analysis and physical determinations such as those of molecular weight. We have therefore greatly expanded the section on Manipulation on a semi-micro scale which was in the Third Edition, and we have described many more preparations on this scale, some independent and others as alternatives to the larger-scale preparations which immediately precede them. Some 40 separate preparations on the semi-micro scale are described in detail, in addition to specific directions for the preparation of many classes of crystalline derivatives required for identification purposes. The equipment required for these small-scale reactions has been selected on a realistic basis, and care has been taken not to include the very curious pieces of apparatus sometimes suggested as necessary for working on the semi-micro scale. 

When collecting a sample, for instance, only a small portion of the available material is taken, increasing the likelihood that small-scale inhomogeneities in the sample will affect the repeatability of the analysis. Individual pennies, for example, are expected to show variation from several sources, including the manufacturing process, and the loss of small amounts of metal or the addition of dirt during circulation. These variations are sources of indeterminate error associated with the sampling process. 

McKelvie, I. D. Cardwell, T. J. Cattrall, R. W. A Microconduit Flow Injection Analysis Demonstration Using a 35-mm Slide Projector, /. Chem. Educ. 1990, 67, 262-263. Directions are provided for constructing a small-scale FIA system that can be used to demonstrate the features of flow injection analysis. For another example see Grudpan, K. Thanasarn, T. Overhead Projector Injection Analysis, Anal. Proc. 1993, 30, 10-12. 

Laboratory experiments using rodents, or the use of gas analysis, tend to be confused by the dominant variable of fuel—air ratio as well as important effects of burning configuration, heat input, equipment design, and toxicity criteria used, ie, death vs incapacitation, time to death, lethal concentration, etc (154,155). Some comparisons of polyurethane foam combustion toxicity with and without phosphoms flame retardants show no consistent positive or negative effect. Moreover, data from small-scale tests have doubtful relevance to real fine ha2ards. 

Bench scale analysis Also known as "bench test . A method of studying different ways of treating wastewater and solids on a small scale in a laboratory. Alken-Murray offers several such test kits including Aiken Clear-Flo Bench Test 1 and Aiken PCB Bench Test. 

FIG. 14 Phase diagram of the quantum APR model in the Q -T plane. The solid curve shows the line of continuous phase transitions from an ordered phase at low temperatures and small rotational constants to a disordered phase according to the mean-field approximation. The symbols show the transitions found by the finite-size scaling analysis of the path integral Monte Carlo data. The dashed line connecting these data is for visual help only. (Reprinted with permission from Ref. 328, Fig. 2. 1997, American Physical Society.)... 

Dimensional analysis techniques are especially useful for manufacturers that make families of products that vary in size and performance specifications. Often it is not economic to make full-scale prototypes of a final product (e.g., dams, bridges, communication antennas, etc.). Thus, the solution to many of these design problems is to create small scale physical models that can be tested in similar operational environments. The dimensional analysis terms combined with results of physical modeling form the basis for interpreting data and development of full-scale prototype devices or systems. Use of dimensional analysis in fluid mechanics is given in the following example. 

The application of a selective pyrolysis process to the recovery of chemicals from waste PU foam is described. The reaction conditions are controlled so that target products can be collected directly from the waste stream in high yields. Molecular beam mass spectrometry is used in small-scale experiments to analyse the reaction products in real time, enabling the effects of process parameters such as temperature, catalysts and co-reagents to be quickly screened. Fixed bed and fluidised bed reactors are used to provide products for conventional chemical analysis to determine material balances and to test the concept under larger scale conditions. Results are presented for the recycling of PU foams from vehicle seats and refrigerators. 12 refs. 

In proper experimentation important considerations are the chemical nature of the analyte and the proposed analytical technique for further analysis of the extract. When a modifier is used it is best to use the modifier solvent as the trapping solvent. A disadvantage of solid-based traps is that most subsequent separation techniques (e.g. GC or HPLC) require a solution consequently, it is then necessary to carry out a (small scale) solvent extraction to remove the analytes from... 

---