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Serum albumin beads

Rayment, R and Butler, M. F. Investigation of ionically crosslinked chitosan and chitosan-bovine serum albumin beads for novel gastrointestinal functionality. J. Appl. Polym. Sci. 108 (2008) 2876-2885. [Pg.295]

Lee, T.K., T.D. Sokoloski and G.P. Royer, Serum albumin beads an injectable, biodegradable system for the sustained release of drugs, Science, 213 (1981) 233-235. [Pg.236]

Proteins. A chiral stationary phase with immobilized a -acid glycoprotein on silica beads was introduced by Hermansson in 1983 [18, 19]. Several other proteins such as chicken egg albumin (ovalbumin), human serum albumin, and cellohy-drolase were also used later for the preparation of commercial CSPs. Their selectivity is believed to occur as a result of excess of dispersive forces acting on the more retained enantiomer [17]. These separation media often exhibit only modest loading capacity. [Pg.58]

For lasalocid assay, polyclonal antibodies were raised in sheep (98). These antisera were applied in an ELISA validated for chicken serum, liver, and muscle. Bridge homology in the ELISA was overcome by absorbing unspecific antisera onto a conjugate between salinomycin and chicken serum albumin, which was immobilized onto Biosilon beads. The assay was highly specific for lasalocid and was capable of detecting it at concentrations less than 0.15 ppb. [Pg.852]

Remove the MTBS from the magnet. Wash the beads with 1 mL TBS and resuspend the beads in the phage solution supplemented with 1% bovine serum albumin (BSA). [Pg.60]

Following the hydrophilic surface modification of the MIP beads, the retention factors of the various employed analytes were decreased, whereas similar or (in the case of (,S )-naproxen) even higher enantioselectivities were observed. Furthermore, the recovery of bovine serum albumin was complete on modified MIPs while it was only 10-40% on unmodified MIPs. Although the column efficiency of modified beads was lower, possibly due to mass transfer limitation by hydrophilic external layers in an aqueous-rich eluent, these preliminary results demonstrated the applicability of RAM-MIPs in direct serum injection assays [95]. [Pg.47]

Column Chromatography. Sepharose beads containing covalently linked gangliosides (0.2 ml packed volume) were placed into a pasteur pipette containing a small amount of glass wool. Columns were washed with HEM containing 50 ug/ml bovine serum albumin (3 ml). Interferon solutions in MEM-albumin (1 ml) were placed on the columns, which were eluted with MEM-albumin at a flow rate of no more than one drop per minute. Fractions of 1 ml were collected and interferon titers determined in each fraction after serial two-fold dilution. Columns onto which mouse fibroblast interferon had been loaded, were eluted with MEM-albumin first, then with 0.07 M N-acetylneuraminyl lactose at pH 2. [Pg.393]

Edwards-Levy, F. and Levy, M.-C. (1999) Serum albumin-alginate coated beads mechanical properties and stability. Biomaterials 20, 2069-2084... [Pg.116]

In order to determine if lectins affect the reactions of complement mediated hemolysis subsequent to binding of antibody to the lymphocyte we have employed a synthetic particulate antigen. This was accomplished by testing the ability of lectin to inhibit the fixation of complement by the complex of anti-human serum albumin (HSA) and HSA-conjugated aminoethyl Biogel beads. The HSA-conjugated aminoethyl Biogel beads may be considered to be cell-like particles coated with a carbohydrate-free protein. [Pg.59]

This method is based on the affinity of the new chemical entity to a membrane, consisting of human serum albumin immobilized on Transil beads. The silica beads are coated with egg yolk phosphatidylcholine and are commercially available under the trademark Transil from Nimbus (Nimbus Leipzig Germany). [Pg.485]

In the fabrication of bead-based glycan arrays, glycopeptides and bovine serum albumin neoglycoproteins were conjugated to the carboxyl-presenting beads using water-soluble carbodi-imides. The beads were spatially arrayed into microwells for... [Pg.43]

Fig. I. Crosman BB shot beads plated with bright nickel and black nickel (for color coding purposes), then coated with polycarbonate by immersing in 5% solution of methylene chloride, scattering on slick paper, and allowing to dry. Various antigens or antibodies are adsorbed onto this plastic surface the beads are soaked in a % solution of bovine serum albumin in phosphate-buffered saline, drained, air dried, and stored below 0° until needed. Fig. I. Crosman BB shot beads plated with bright nickel and black nickel (for color coding purposes), then coated with polycarbonate by immersing in 5% solution of methylene chloride, scattering on slick paper, and allowing to dry. Various antigens or antibodies are adsorbed onto this plastic surface the beads are soaked in a % solution of bovine serum albumin in phosphate-buffered saline, drained, air dried, and stored below 0° until needed.
A water-insoluble lactoperoxidase, linked to CNBr-activated Sepharose beads, capable of iodinating serum albumin, is commercially available (Worthington Biochemical Corp.), and may be useful where avoidance of contamination of the product by the enzyme is important. [Pg.101]

One of the earliest efforts of qualitative measurement of a protein (human serum albumin) in a microchip-based device was based on bead agglutination in a microchamber (approximately lOjaL). Subsequently, several quantitative immunoassays have been performed using microchip electrophoretic systems that permit separation and quantitation of free- and bound-labeled antigens in competitive assays (see Chapter 5). Most are carried out in channels micro-machined into fused silica substrates. Early work on quantitative assays achieved measurement of cortisol in serum.The assay used cortisol labeled with fluorescein and an argon laser detector at 488 nm and required only 80 pL of a 40x dilution of serum as the sample. Other capillary electrophoresis-based assays for a variety of antibodies have also been developed that include immunoglobulins (IgG, IgA, and IgM), antibovine serum albumin, and antiestradiol. ... [Pg.255]

Collect beads by centrifugation for 30 sec at 1500 g, and wash beads five times in 800 pi binding buffer (without RNasin, bovine serum albumin and tRNA).c... [Pg.104]

We also reported a concept for a sandwich immunoassay that is completely performed on-chip using streptavidin-coated beads as substrate [17]. The latter were electrostatically self-assembled on aminosilane micropattems at the bottom of a microfluidic channel. We used mouse IgG diluted in phosphate buffered saline (PBS) with 1% bovine serum albumin (BSA) solution as target antigen. The fluorescent sandwich immunocomplex was formed on the beads during the operation of the chip both in stop-flow and continuous flow modes. Target mouse IgG antigen could be detected down to a concentration of 15 ng/mL in stop-flow mode and 250 pg/mL in continuous flow mode, using only 1,300 nL of sample volume. [Pg.459]

This method is based on the accessibility of the pores in the stationary phase for 99mrpc-iabeled molecules of different molecular sizes. The sample is eluted from a vertical column packed with porous beads of the gel by gravity or low pressure. Smaller Tc species penetrate the pores and are retained on the column, while larger molecules are excluded and are therefore rapidly eluted from the column. This separation technique has particular application for macromolecules, proteins (serum albumin, immunoglobulins [e.g., monoclonal antibodies and their fragments]), but has also been used for separation of small-molecular-weight Tc-diphosphonate complexes. [Pg.137]


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See also in sourсe #XX -- [ Pg.218 ]




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