Chicken serum

Halofuginone Chicken serum Bond-Elut Cg HgO/HOAc, MeOH- HgO/ACN/HOAc 315... 

For lasalocid assay, polyclonal antibodies were raised in sheep (98). These antisera were applied in an ELISA validated for chicken serum, liver, and muscle. Bridge homology in the ELISA was overcome by absorbing unspecific antisera onto a conjugate between salinomycin and chicken serum albumin, which was immobilized onto Biosilon beads. The assay was highly specific for lasalocid and was capable of detecting it at concentrations less than 0.15 ppb. 

Halofuginone can be also analyzed in chicken serum by a competitive ELISA developed on the basis of monoclonal antibodies (99). In this study, a serum matrix effect that afforded a higher sensitivity for the detection of halofuginone in chicken serum than in assay buffer or in highly diluted serum was observed. The sensitivity of the ELISA improved when used in more concentrated serum. 

Szepfalusi, Z., Ebner, C., Pandjaitan, R., Orlicek, F., Scheiner, O., Boltz-Nitulescu, G., Kraft, D., Ebner, H. 1994. Egg yolk alpha-livetin (chicken serum albumin) is a cross-reactive allergen in the bird-egg syndrome. J Allergy Clin Immunol 93 932-942. 

G3. Green, R., Newmark, P. A., Musso, A. M., and Mollin, D, L., The use of chicken serum for measurement of serum vitamin B12 concentration by radioisotope dilution Description of method and comparison with microbiological assay results. Br. J. Haematol. 27, 507-527 (1974). 

The transferrins contain varying amounts of carbohydrates (Table 7) and can be classified as glycoproteins. Williams (136) stated that ovotrans-ferrin and chicken serum transferrin differed only in carbohydrate contents. The ovotransferrin contains no sialic acid (44, 136). Serum transferrin, on the other hand, contains sialic acid. Williams (136), from the results of treating the serum transferrin with the enzyme neuraminidase, suggested that differences in the electrophoretic mobilities and isoelectric points of the multimolecular forms of serum transferrins may be due to differences in their contents of sialic acid. 

Table 1. Amino acid composition of chicken ovotransferrin and chicken serum...

II. Chicken ovo- III. Chicken serum IV. Bovine lacto- V. Human lacto-... 

The relationships among the transferrins in the different fluids from the same individual are of obvious interest. Unfortunately, only limited data are available on this subject. A close immunological relationship of chicken serum transferrin and chicken ovotransferrin has been reported many times (20, 65, 79, 92, 136). Williams (136) in a detailed study by immunological methods, peptide mapping, and amino acid analyses reported that chicken serum transferrin and chicken ovotransferrin differ only in their carbohydrate prosthetic groups. 

Centrifuge the pooled liquid for 15-20 min at 1500 y at 4°C. Aspirate the clear serum and store at-20 C (or-70 C). Alternatively, serum can be stored at 4°C if an antibacterial agent (e.g., 0.02% NaNj) is added. Do not freeze chicken serum or IgG because the immunoglobulins are particularly sulyect to damage. 

Various types of serum are fetal bovine serum—the most widely used type, newborn calf serum— which is derived from animals less than 10 days of age, donor calf serum—which is obtained from processed whole blood of calves up to 8 mo old, and horse serum. Human serum and chicken serum have specialized uses for those cells that require a serum derived from similar species, e.g., chicken serum has been repeatedly used for growing various types of avian cells. Serum can be stored safely for over 12 mo at —20°C and longer storage is possible at —70°C. Serum can also be heat inactivated (incubation at 56°C for 30 min) to remove toxic compounds or other agents that can interfere with tissue typing assays. 

DIO. Deutsch, H. F., Nichol, J. C., and Cohn, M., Biophysical studies of blood plasma proteins. XI. Immunological and electrophoretic studies of immune chicken serum. J. Immunol. 63, 195-210 (1949). 

Egg yolk portion a-Livetin (chicken serum albumin) Gald 5 70 3.100 47.0 ... 

Among recent developments in glycoconjugates appHed for drug immunotherapy and so on [140], a variety of oligosaccharide-squaramide-protein (e.g., bovine, human, and chicken serum albumins) conjugates have been prepared with the Tietze s squarate ester methodology and tested for their bioactivity [141-157]. The glycoconjugates with special functions were also docimiented for example, 1,4,7,10,13-pentaazacyclopentadecane... 

Trypsin solution Dissolve 250mg of trypsin (Difco, Surrey, UK) and 372mg EDTA disodium salt (Sigma) in 1L of PBS. Add lOmL of chicken serum (Flow Labs, Herts, UK and filter sterilize. Store in 20-mL aliquots at -20°C. 

Svennebholm and Svennebeolm [161, 162] have extracted the neutral glycohpids from human serum, spleen and hver and were able to separate 4 different glycohpids, ascertaining their structure and distribution. Faillabd and Cabezas [42] give a method for isolation of N-acetyl-and N-glycolylneuraminic acid from calf and chicken serum. 

Obtain about 100 mg of animal tissue or 100 pi of animal fluid (suggested materials are mouse, frog, or snail liver human or chicken serum or snail hemolymph). 

The sedimentation coefficient of different preparations of chicken sIgA, isolated from secretions, varied from 11 S to 16 S (98). IgA has also been identified by Leslie and Martin (99) in chicken serum, where it is present at low concentration. The serum protein, which reacts with antiserum to chicken sIgA, exists mainly in a polymeric form but is transformed into the monomer (mol. wt. 175,000) upon mild reduction. The molecular weight of the chicken a chain is about 70,000. IgA from chicken bile contains an additional antigenic determinant, as compared to serum IgA, which might be present on secretory component or J chain (99). 

Sogorb, M.A., Monroy, A., Vilanova, E., 1998. Chicken serum albumin hydrolyzes dichlorophenylphosphoramidates by a mechanism based on transient phosphorylation. Chem. Res. Toxicol. 11 (12), 1441-1446. 

QT6, stable and permanent cell line from methylcholanthrene-induced pectoralis fibrosarcomas of Japanese quail Cotumix cotumix Japonica developed by Antin and Ordahl, was used in this study. QT6 cells were grown in McCoy medium supplemented with 10% foetal calf serum and 1 % chicken serum to 80-90% confluence, fasted 16 h in serum-free medium, washed once with PBS and incubated for 2 h in different media varying in glucose (1 to 3 g/1 of glucose) and/or amino acid concentrations (0.5x to 2x). Cell samples were stored at -80 °C until further analysis. 

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