Neoglycoproteins bovine serum albumin

The latter approach has been followed in our laboratory. For instance, in studies related to the synthesis of potent multivalent sialoside inhibitors of influenza virus hemagglutinin [34], it became of interest to evaluate the immunogenicity of neoglycoproteins containing sialosides as sole immunodominant hapten. To this end, acetochloroneuraminic acid (1) was transformed into allyl glycoside 2 (Scheme 1) which upon reductive ozonolysis afforded aldehyde 3 in excellent overall yields [35], Reductive amination of 3 to bovine serum albumin (BSA) and tetanus toxoid provided immunogenic vaccines from which specific rabbit IgG anti-sialic acid antibodies were obtained [36]. In order... 

In the fabrication of bead-based glycan arrays, glycopeptides and bovine serum albumin neoglycoproteins were conjugated to the carboxyl-presenting beads using water-soluble carbodi-imides. The beads were spatially arrayed into microwells for... 

The specificity of the antiserum raised against the Lea neoglycoprotein (9) was determined by hapten-inhibition studies using synthetic oligosaccharides,72 and derivatives of bovine and horse serum-albumin to which the Lea trisaccharide had been attached.73 On the basis of hapten inhibition of precipitation, this antiserum was shown to be highly specific for the carbohydrate portion of the synthetic antigen,... 

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