Streptavidin using

S. Miyamoto and P. A. Kollman. Absolute and relative binding free energy calculations of the interaction of biotin and its analogs with streptavidin using molecular dynamics/free energy perturbation approaches. Proteins, 16 226-245, 1993. 

Miyamoto S and P A Kollman 1993a. Absolute and Relative Binding Tree Energy Calculations of the Interaction of Biotin and its Analogues with Streptavidin Using Molecular Dynamics/Free Energy Perturbation Approaches. Proteins Structure, Function and Genetics 16 226-245. 

Radioiodination of streptavidin uses procedures similar to those used for stable nuclides However, some distinct differences remain, since radioiodinations are... 

To purify the SATA-modified avidin or streptavidin use gel filtration on a column of Sephadex G-25 or dialyze against 0.1 M sodium phosphate, 0.15 M NaCl, pH 7.2, containing 10 mM EDTA. At this point, the derivative is stable and may be stored under conditions that favor long-term avidin activity. 

For each 20 cm x 7.5-8.0 cm membrane section, prepare 50 g.L of a 20 pM streptavidin and 60 pM biotinylated capture probe mixture in 0.4M NaHC03/Na2C03, pH 9.0 containing 5% (v/v) methanol. We have found that an unmodified capture probe may also be immobilized without streptavidin using the same conditions with both suggested membrane types. However, the efficiency of the immobilization with and without streptavidin should be compared for other membrane types. 

The association constants between strep-tavidin and the biotin group were determined from the study of the construction of protein-DNA double and triple layers (1st layer biotinylated hpid monolayer, 2nd streptavidin, 3rd biotinylated lipid double-strand oligonucleotide, 4th streptavidin) using a 9-M Hz QCM, and found to be 4.8 X 10 3.2 x 10 and 3.6 x lO M ... 

Ding Z, Fong RB, Long CJ, Stayton PS, Hoffinan AS (2001) Size-dependent control of the binding of biotinylated proteins to streptavidin using a polymer shield. Nature 411 59-62... 

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