Saline, heparinized

Inject a 2 % EB solution (3 mL/kg) in the mouse tail vein or jugular vein. Then anesthetize animal and draw blood by cardiac puncture followed by transcardial perfusion with 0.9 % saline-heparin to remove the intravascular dye. In fresh sections of the CNS, if the BBB is compromised, EB staining can be seen as a blue area in a cross section of the brain. The sample can then be frozen for immunofluorescence. 

The primary advantages of implantable ports are no maintenance between uses other than periodic flushing with heparinized saline every 28 days to ensure patency, lower incidence of clotting and thrombosis, no dressing changes, insignificant infection incidence, unobtmsive cosmetic appearance, and no restriction on physical activity. 

The infusion system was similar to that described by Findley et al. (1972). The catheter was attached to a valve system that allowed slow continuous administration (55 to 60 mL in 24 hours) heparinized saline (5 units/mL) via a peristaltic pump to maintain catheter patency. Drug was injected into the valve system by means of a second pump and then flushed into the animal with 5 mL of saline from a third pump. This system necessitated a delay of approximately 20 seconds between the onset of drug delivery and actual injection into the vein. Drugs were delivered within a 2-minute period. 

Another important bioanalytical application of voltammetric ISEs is the detection of polyions (see also above). A technique using cyclic voltammetry on micropipette electrodes filled with the organic electrolyte solutions in 1,2-dichloroethane was successfully applied for the detection of protamine [65] in saline solution and heparin in undiluted sheep plasma samples [66]. Protamine transport was facilitated with dino-nylnaphthalenesulfonic acid (DNNS). As a heparin-selective component the tetrakis-(4-chlorophenyl)borate salt of trimethyloctadecyl ammonium was used. 

Calculate the number of units per milliliter if a 4 mL vial of heparin containing 10,000 units/mL is injected into 1000 mL of normal saline solution. 

The cholinesterase-inhibiting activity of the phosphorofluoridates was compared quantitatively with that of eserine sulphate thus. To 0-2 ml. of heparinized human plasma was added 05 ml. of a solution containing either eserine or the phosphorofluoridate in varying concentrations then the mixture was kept at room temperature for 10 min. before 1 /tg. of acetylcholine in 1 c.c. saline solution was added. After 5 min. at room temperature, the mixture was made up to 10 ml. with frog saline containing eserine 1/100,000, which at once stopped the action of any cholinesterase not yet inactivated. The solution was then assayed for acetylcholine on the frog rectus-muscle preparation. 

Plasma non-esterified fatty acids (NEFA) were elevated by infusion of a lipid emulsion and heparin with a constant rate of 1.5 ml/min (lipid emulsion, protocols [1] and [3]) and 0.4 lU/kg per minute (heparin). In protocol [2] a solution of 0.9% saline was infused as a control for the lipid emulsion. 

Figure I. Photomicrograph of heparin-induced BBC aggregation. Human RBCs were washed three times with tris-bufferea saline (6) and resuspended in the buffered saline containing 2 g % heparin (Sigma Chemical Co.). RBC9s formed typical rouleaux.

At respective intervals as described above the monkeys were euthanized by lethal doses of sodium pentobarbital, the chest was opened, and intracardial perfusion of 0.51 ice-cold saline with heparin was performed, immediately followed by 2.51 of ice-cold solution of 4% paraformaldehyde in phosphate-buffered saline (PBS), pH = 7. The cranium was opened, and the brain was removed. 

A. Wright, and J. Hecker, Long term stability of heparin in dextrose saline intravenous fluids. Int. J. Pharmacol. Pract. 3 253-255, 1995. 

In order to estimate the release of heparin from the PVC matrix in blood, the amount and the rate of release of heparin from the PVC matrix were examined at 37 °C in physiological saline. Figure 23 shows the release behavior of heparin from PVC films containing heparin-immobilized hydrogels. Too much immobilized heparin in PVC is not well sustained, however, and suitable amounts... 

The Arrow implantable pump is non-programmable and delivers infusate (2-deoxy-5-fluorouridine, morphine sulfate, baclofen, or heparinized saline) at 3 pre-set flow rates. The pump is divided into two chambers by accordion-like movable bellows. Infusate is placed in the inner dmg reservoir chamber and Freon propellant in the outer chamber (Figure 4.19). 

Remove a small strip of skin from the abdomen and make a small incision in the peritoneal wall. Inject 35 mL of heparinized saline (10 IU/mL) and gently massage the abdomen for 20 s. Extend the incision and harvest the cell suspension. 

The permanency of the bonded heparin on a number of selected polymer systems has been measured using radiolabeled heparin (35S). It has been found that the loss of heparin after prolonged exposure to distilled water and isotonic saline at 37 °C. is almost negligible for heparinized polypropylene, silicone rubber, and Hydrin rubber. On the other hand, polypropylene loses 70% of its originally bound heparin after exposure to plasma for three hours. Hydrin rubber and silicone rubber lose only from 0 to 5%. The loss of heparin on the polypropylene may be attributed to displacement of the heparin molecules by a protein or combination of proteins. A series of experiments was performed in which... 

An important factor in the interaction of foreign surfaces with blood is the rapid adsorption of plasma proteins onto such surfaces when they are exposed to blood (4). For this reason the adsorption of radioactively tagged blood components on heparinized and unheparinized surfaces was measured. Proteins were dissolved in approximate physiological concentrations in a buffered (pH 7.35) physiological saline solution and the solutions were exposed to the test surfaces for 2 hours at 37 °C. in a static system. After the exposure, the surfaces were rinsed with physiological saline and distilled water and then dried. The amount of protein on the surfaces was determined in a 27r-gas flow proportional counter (7). As shown in Table III, although both heparinized surfaces were nonthrombogenic, there is no consistent pattern of either increased or decreased adsorption of the proteins caused by the heparinization. In-... 

Blood Heparinization transfer to isotonic saline in headspace vial equilibration with heat HRGC/PID 0.4 pg/L NR Pekari et al. 1989... 

Three patients developed priapism after taking cocaine or non-prescription weight loss formulations containing ephedrine (216). Intracavemous injection of phenylephrine and irrigation with heparinized saline, followed by an Al-Ghorab shunt procedure, was effective. 

A syringe is prepared with heparinized saline and a 21-, 23-, or 25-gauge scalp-vein needle, and the patient is phlebotomized, usually in the antecubital vein. Fluorescein may also be injected into the veins of the back of the hand or wrist. The needle must be monitored, because extravasation of dye results in localized pain. Before injecting fluorescein the photographer takes baseline color photographs and then red-free fundus pictures. 

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