Saccharomyces growth

Juice and wine pH play a mjgor role in the development of yeast and bacterial populations. Species respond differently in terms of growth rate as well as production of sensorially important metabohtes. In the case of Saccharomyces, growth and fermentation rates slow as pH decreases to near 3.0, concomitantly increasing the risk of sluggish or stuck fermentations. Kudo et al. (1998) found that the relative concentration of and H in a grape must plays an important role in successful completion of alcoholic fermentation, with a minimum of 25 1 being necessary. Whereas... 

Trehalose is particularly well-suited for this purpose and has been shown to be superior to other polyhydroxy compounds, especially at low concentrations. Support for this novel idea comes from studies by P. A. Attfield, which show that trehalose levels in the yeast Saccharomyces cerevisiae increase significandy during exposure to high salt and high growth temperatures—the same conditions that elicit the production of heat-shock proteins ... 

Dining batch fermentation of Saccharomyces cerevisiae, other influential parameters can adversely influence the specific rate of growth, and inhibition can be caused either by... 

Fig. 10.2. Standard curve for cell density define based on growth of Saccharomyces cerevisiae.

Example 12.7 Develop a model for the anaerobic batch fermentation of glucose to ethanol and coproduct CO2 using Saccharomyces cerevisiae. The starting mixture contains 10% glucose. The inoculum is 0.0005 w/w. Product inhibition stops cell growth at 14% ethanol. Assume ka = 0 but include the cannibalization of cellular material beginning when the substrate is completely consumed. 

Fig. 2.3 The development of polarity and asymmetric division in Saccharomyces cerevisiae. The diagram is reproduced in a slightly simplified form from the work of Lew Reed (1995) with the permission of Current Opinion in Genetics and Development, (a) The F-actin cytoskeleton strands = actin cables ( ) cortical actin patches, (b) The polarity of growth is indicated by the direction of the arrows (arrows in many directions signifies isotropic growth), (c) 10-nm filaments which are assembled to form a ring at the neck between mother and bud. (d) Construction of the cap at the pre-bud site. Notice that the proteins of the cap become dispersed at the apical/isotropic switch, first over the whole surface of the bud, then more widely. Finally, secretion becomes refocussed at the neck in time for cytokinesis, (e) The status and distribution of the nucleus and microtubules of the spindle. Notice how the spindle pole body ( ) plays an important part in orientation of the mitotic spindle.

Andreasen A. A. Stier J.B. (1953) Anaerobic nutrition of Saccharomyces cerevisiae. I. Ergosterol requirement for growth in a defined medium. / Cell Comp Physiol, 41, 23-36. 

Wright R.M., Repine T. Repine J.E. (1993) Reversible pseudohyphal growth in haploid Saccharomyces cerevisiae is an aerobic process. CurrGenet, 23, 388-391. 

Kuranda, M.J. and Robbins, P.W. (1991) Chitinase is required for cell separation during growth of Saccharomyces cerevisiae. Journal of Biological Chemistry 266, 19758-19767. 

Weeks et al. 1979) or DNA damage (Nakamura et al. 1987) following hexachloroethane treatment. Similar results were reported for eukaryotic cells. Hexachloroethane did not cause gene mutation in cells harvested from the stationary growth phase (Bronzetti et al. 1989) or DNA damage in yeast (Saccharomyces cerevisiae) (Simmon and Kauhanen 1978), chromosomal aberrations in fungi (Aspergillus nidulans) (Crebelli et al. 

Adams AE, Pringle PR. Relationship of actin and tubulin distribution to bud growth in wild-type and morphogenetic-mutant Saccharomyces cerevisiae. J Cell Biol 1984 98 934-945. 

The bakers yeast Saccharomyces cerevisiae represents probably the currently best researched and understood eukaryotic organism. Most of its genes have been knocked out for functional studies, and hence a vast variety of mutant strains as well as tools for their manipulation are available. This in concordance with short generation times and a growth medium that is comparable in cost and complexity to bacterial media has made the yeast system a frequent choice for the evaluation of transmembrane carriers. 

The gene that encodes H2A.Z is essential in Drosophila [42], Tetrahymena [43], and mice [44]. Deletion of the gene that encodes H2A.Z in yeast results in slow growth and chromosome segregation defects in Schizosaccharomyces pompe [38], and in slow growth and defects in gene regulation in Saccharomyces cerevisiae [45-48]. 

It is interesting to note that, while Saccharomyces and Candida quorum sensing signals share similarity, their effects seem different. In Saccharomyces, the signals lead to adhesion and invasive filamentous growth in Candida, filamentation is suppressed. Farnesol seems to promote the dispersion of cells from the Candida biofilm. ... 

Mishra, I.M., El-Temtamy, S.A. and Schugerl, K., Growth of Saccharomyces cere-visiae in gaseous fluidized beds, Eur.. Appl. Microbiol. Biotechnol., 16 (1982) 197-203. 

Maynard, A.I., The influence of magnesium ions on the growth and metabolism of Saccharomyces cerevisiae, PhD thesis, Dundee Institute of Technology, 1993. 

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