Saccharomyces cerevisiae haploid

Saccharomyces cerevisiae haploid strain his3 derived firom BY4741 (access number Y02458 MAT his3 KanMX ku2 metis um3) (wNote 1). 

Fig. 2.4 The budding pattern in haploid and diploid Saccharomyces cerevisiae. The original cell which formed a bud is the mother (M). The daughter cell (D) is shown remaining attached as might be the case in i colonies growing on the surface of agar.

Fig. 2.6 The moqjhological events of sporulation in Saccharomyces cerevisiae. (a) starved cell V, vacuole LG, lipid granule ER, endoplasmic reticulum CW, cell wall M, mitochondrion S, spindle pole SM, spindle microtubules N, nucleus NO, nucleolus, (b) Synaptonemal complex (SX) and development of polycomplex body (PB) along with division of spindle pole body in (c). (d) First meiotic division which is completed in (e). (f) Prepararation for meiosis II. (g) Enlargement of prospore wall, culminating in enclosure of separate haploid nuclei (h). (i) Spore coat (SC) materials produced and deposited, giving rise to the distinct outer spore coat (OSC) seen in the completed spores of the mature ascus (j). Reproduced from the review by Dickinson (1988) with permission from Blackwell Science Ltd.

Wright R.M., Repine T. Repine J.E. (1993) Reversible pseudohyphal growth in haploid Saccharomyces cerevisiae is an aerobic process. CurrGenet, 23, 388-391. 

Aspergillus nidulans haploid, mutation Aspergillus nidulans non-disjunction Aspergillus nidulans, mitotic crossing-over Saccharomyces cerevisiae, forward mutation... 

Mortimer, R.K. (1958). Radiobiology and genetic studies on a polyploid series (haploid to hexaploid) of Saccharomyces cerevisiae. Radiation Res. 9,312-332. 

Saccharomyces cerevisiae played an important role in the genetic analysis of riboflavin biosynthesis. Mating analysis between riboflavin auxotrophic mutants obtained by random mutagenesis and screening of haploid... 

Saccharomyces cerevisiae (baker s yeast, haploid strain X2180 [39]) [8, 33, 39-42]... 

In a study on haploid and diploid cells of Saccharomyces cerevisiae, it was shown that the haploid cells are more susceptible to the action of furanocoumarins under 365 nm light, which is probably due to different repair capacity [284]. 

Methyl methanethiosulfonate was negative in chromosomal aberration assays conducted in Saccharomyces cerevisiae strain D7 or S. cerevisiae haploid strain N123 at concentrations of up to 300 pg/ml (Dorange et al., 1983). 

Figure 9.9 A schematic representation of the cell cycle of a budding wine yeast cell. Haploid and diploid cells reproduce asexually by multilateral budding during which each cell gives rise to a daughter cell made of entirely new cell surface material. Buds may arise at any point on the mother cell surface, but never again at the same site. Under optimal nutritional and cultural conditions Saccharomyces cerevisiae doubles its mass every 90 min. The cell division cycle consists of four phases Gl, S, G2 and M.

Most work in yeast genetics has been performed with two species, Sac-char omyces cerevisiae and Schizosaccharomyces pombe. For a detailed description of the respective life cycles, see Mortimer and Manney in Volume 1 of this series. It must be pointed out that both organisms can be cultivated as haploids and, in the case of Sacch. cerevisiae, in stable diploid forms as well. Since there are a great variety of yeasts of the Saccharomyces type which do not behave in this ideal way, all genetic experiments should be performed with strains currently used by yeast geneticists. Such strains are physiologically dioecious (see Esser 2) for definition of this term), whereas many strains isolated from nature are self-compatible and haploid cells fuse uncontrollably to form diploids. Typically, Saccharomyces strains... 

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