Saccharomyces cerevisiae, growth rate

Sato and Yoshizawa [119] modeled the production of ethanol, a catabolic end product, during SSF of Saccharomyces cerevisiae. The rate of ethanol production was assumed to be directly proportional to the rate of carbon dioxide formation, which had both growth and non-growth associated terms as shown in Eq. (18). The non-growth associated term represented maintenance metabolism and the non-growth associated rate constant was assiuned to decrease exponentially as the ethanol concentration increased, in order to describe the inhibitory effect of ethanol on its own production. 

Dining batch fermentation of Saccharomyces cerevisiae, other influential parameters can adversely influence the specific rate of growth, and inhibition can be caused either by... 

We have studied CNT influence on growth rate and proliferation of some cellular colonies [29]. Interesting results were obtained in case of bread-making yeast-like fungi Saccharomyces cerevisiae (strain 608) and hamster kidney cells. Introduction of small amounts of CNT ( 3 pg/mL) in fungal suspensions led to 2-fold increase in Saccharomyces cerevisiae colonies number compared to the control, after 48 h of incubation at 30°C (Fig. 2.2). Similar results were obtained for colonies of hamster kidney cells. Presence of CNT activated cell proliferation and increased cell growth rate by 1.5 times. 

According to a review by Walsh [10], of 165 biopharmaceuhcal products approved in the United States and Europe by 2006, only two are nucleic acid-based drugs, whereas nine of the 31 therapeutic proteins approved since 2003 are produced in E, coli, and 17 are produced by mammalian cell lines. In 2004 market distribution and manufacture of therapeutic proteins, non-glycosylated (non-antibody) proteins constitutes 40% of the total market, with 12% armual growth rate, and are produced in E. coli or the yeast Saccharomyces cerevisiae glycoproteins (primarily mAbs) constitute 60% of the total market, with 26% armual growth rate, and are produced by mammalian cell culture (mostly with cells from Chinese Hamster Ovary, or CHO). 

Parker, C. and D. DiBiasio, "Effect of Growth Rate and Expression Level on Plasmid Stability in Saccharomyces cerevisiae Biotech. Bioeng. 29 (1987) 215-221. 

Curves of cells of Saccharomyces cerevisiae yeast growth in suspension at various concentrations of nickel (II) sulfate may serve as an example (Figure 1) [14], These curves are the evidence of toxic action of nickel (II) ions. Increase of toxicant concentration leads to decrease of rate during the period of exponential growth (parameter p in equation (7)) and maximum size of population of yeast (Parameter p/a in equation(7)) at one and the same initial size of yeast population, N0=100 cells/ml. 

Glucose-to-ethanol fermentation is to be carried out in a batch reactor using an organism such as Saccharomyces cerevisiae. Plot the concentrations of cells, substrate, and product and growth rates as functions of time. The initial ceil concentration is 1.0 g/dm and the substrate (glucose) concentration is 250 g/dm. ... 

The saturation constant for Saccharomyces cerevisiae on glucose is 25 mg/L, for Escherichia on lactose 20 mg/L, and for Pseudomonas on methanol 0,7 mg/L. Here, is the maximum growth rate achievable when... 

FIGURE 8.12 Relative growth rate (arbitrary scale) of some microorganisms as a function of water activity (aw). (a) A xerophilic mold, Xeromyces bisporus. (b) A common mold, Aspergillus flavus. (c) A yeast, Saccharomyces cerevisiae. (d) A bacterium, Salmonella sp. 

Illanes A, Altamirano C, AUlapan A et al. (1998) Packed-bed reactor performance with immobilized lactase under thermal inactivation. Enzyme Microb Technol 23 3-9 Impoolsup A, Caunt P, Greenfield PE (1989) Effect of growth rate on stability of a recombinant plasmid during continuous culture of Saccharomyces cerevisiae in non-selective medium. J Biotechnol 10(2) 171-180... 

Yeast was the first microbial host used by mankind for biotransformation of raw materials, and it marked the early developments of industrial biotechnology. Initially, Saccharomyces cerevisiae and closely related species were used because of their high fermentative capacity and based on the vast experience from alcoholic beverage production. While a high fermentation rate is favorable for the production of bioethanol and other primary metabolites, it implicates disadvantages for growth-coupled production. Consequently, a number of other yeasts have been developed for the production of biofuels, biochemicals, lipids, or recombinant proteins. 

Rates of fermentation, respiration, and growth, of Saccharomyces cerevisiae on ... 

Mas, S., Ossard, F, Ghommidh, C. (2001). On-line determination of flocculating Saccharomyces cerevisiae concentration and growth rate using a capacitance probe. Biotechnology Letters, 23, 1125-1129. 

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