Saccharomyces cerevisiae mutants

Jones DL, Petty J, Hoyle DC, Hayes A, Ragni E, Popolo L, Oliver SG, Stateva LI (2003) Transcriptome profiling of a Saccharomyces cerevisiae mutant with a constitutively activated Ras/cAMP pathway. Physiol Genomics 16 107-118... 

Biosynthesis.—Ubiquinone. The identification of 3,4-dihydroxyhexaprenylben-zoate (162) in a Saccharomyces cerevisiae mutant strain that cannot synthesize ubiquinone suggests that (162) may be an intermediate in ubiquinone-6 biosynthesis in eukaryotes, in contrast to the pathway via 2-polyprenylphenol which operates in prokaryotes. In mammalian systems alternative routes have been discussed for ubiquinone biosynthesis in rats." Some properties of mitochondrial 4-hydroxybenzoate-polyprenol transferase have been described."" ... 

Invertase from a Saccharomyces cerevisiae mutant could be separated into two fractions on the basis of solubility in ammonium sulfate.382 The soluble fraction reacted with endo-(l - 6)-a-mannanase, when it became insoluble. The results suggested that the insoluble fraction contained only the highly branched, core section, but the soluble fraction also had the (l->6)-a-D-mannan chain attached. 

Venkov, P.V., Milchev, G.I., Hadjiolov, A.A. Rifampin susceptibility of RNA synthesis in a fragile Saccharomyces cerevisiae mutant. Antimicrob. Ag. Chemother. 1975, 627... 

Klig, L.S., Homann, M.J., Kohlwein, S.D., Kelley, M.J., Henry, S.A., and Carman, G.M., 1988a, Saccharomyces cerevisiae mutant with a partial defect in the synthesis of CDP-diacylglycerol and altered regulation of phospholipid biosynthesis. J Bacteriol. 170 1878-1886. 

Saccharomyces cerevisiae (mutant resistant to 2-amino-4-methyl-5- -hy-droxyethylthiazole, an antimetabolite of 4-methyl-5-/l-hydroxyethylthia-zole, deficient in activity of both EC 2.5.1.3 and EC 2.7.1.50 [2] bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity [2]) [1, 2]... 

Kelm and Nair detected the presence of parthenolide in the hexane extract of M. salicifolia fruits by HPLC [11]. Parthenolide (1) and a related compound, costunolide (2), had shown topoisomerase I (top-l) inhibitory activity (refer to Bioassay Procedures section, Topoisomerase inhibitory bioassays) against Saccharomyces cerevisiae mutant strains hypersensitive to top- poisons [12] (Table 1). The isolation of parthenolide from M. grandiflora [13, 14, 15] and M sieboldii spp. japonica and M. praecoissima a.r. praecoissima [16] is discussed in other studies as well. Costunolide was isolated from the trunk and root bark ether extracts of M sieboldii [17, 18]. Also, parthenolide and costunolide, were isolated from Tanacetum parthenium Asteraceae [19]. The extraction of these compounds by supercritical CO2 (SFCO2) and near critical propane from M. grandiflora has also been carried out [20]. 

Sec7 Domain. The SECT protein was identified in a genetic screen of Saccharomyces cerevisiae mutants for defects in protein secretion (Achstetter et al., 1988). Structurally similar domains that were later recognized in other proteins involved in vesicular trafficking were referred as See domains and subsequently shown to be responsible for ARF activation (Morinaga et al., 1996 Peyroche et al., 1996) as well as its sensitivity to... 

Terrel, S. L., Bernard, A., and Bailey, R. B. (1984). Ethanol from whey Continuous fermentation with a catabolite repression-resistant Saccharomyces cerevisiae mutant. Appl Environ Microbiol. 48, 577-580. 

Added support for the supposition that the biosynthesis of riboflavin and cyanocobalamin make use of a common precursor comes from the increased synthesis of riboflavin by Ashbya gossypii in cobalt-supplemented media (Hickey, 1954) and from the stimulation of riboflavin formation in a Saccharomyces cerevisiae mutant by purines, glycine, and methionine (Giri and Krishnswamy, 1964). 

Dinardo S, Voelkel K, Stemglanz R 1984 DNA topoisomerase II mutant of Saccharomyces cerevisiae. topoisomerase II is required for segregation of daughter molecules at the termination of DNA replication. Proc Natl Acad Sci USA 81 2616-2620... 

Adams AE, Pringle PR. Relationship of actin and tubulin distribution to bud growth in wild-type and morphogenetic-mutant Saccharomyces cerevisiae. J Cell Biol 1984 98 934-945. 

The bakers yeast Saccharomyces cerevisiae represents probably the currently best researched and understood eukaryotic organism. Most of its genes have been knocked out for functional studies, and hence a vast variety of mutant strains as well as tools for their manipulation are available. This in concordance with short generation times and a growth medium that is comparable in cost and complexity to bacterial media has made the yeast system a frequent choice for the evaluation of transmembrane carriers. 

Our approach recently developed utilizes DNA repair- or recombination-deficient mutants of the yeast Saccharomyces cerevisiae [8]. An important feature of many tumor cells is that they have defects in their ability to repair damage to DNA as compared with normal cells, suggesting that agents with selective toxicity towards repair-deficient cells might be potential anticancer agents. 

Raths, S., Rohrer, J., Crausaz, F. and Riezman, H., 1993, Two mutants defective in receptor-mediated and fluid-phase endocytosis in Saccharomyces cerevisiae. J.Cell Biol., 120 55-65. 

Sudarsanam, P., Iyer, V.R., Brown, P.O., and Winston, F. (2000) Whole-genome expression analysis of snf/swi mutants of Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 97, 3364-3369. 

Verhage, R.A., van Gool, A.J., de Groot, N., Hoeijmakers, J.H., van de Putte, P., and Brouwer, J. (1996) Double mutants of Saccharomyces cerevisiae with alterations in global genome and transcription-coupled repair. Mol. Cell. Biol. 16, 496-502. 

DePace AH, Santoso A, Hillner P, Weissman JS (1998) A critical role for amino-terminal glu-tamine/asparagine repeats in the formation and propagation of a yeast prion. Cell 93 1241-1252 Didichenko SA, Ter-Avanesyan MD, Smirnov VN (1991) Ribosome-bound EF-1 alpha-like protein of yeast Saccharomyces cerevisiae. Eur J Biochem 198 705-711 Dong H, Kurland CG (1995) Ribosome mutants with altered accuracy translate with reduced processivity. J Mol Biol 248 551-561... 

Stansfield 1, Jones KM, Kushnirov VV, Dagkesamanskaya AR, Poznyakovski Al, Paushkin SV, Nierras CR, Cox BS, Ter-Avanesyan MD, Tuite ME (1995) The products of the SUP45 (eRFl) and SUP35 genes interact to mediate translation termination in Saccharomyces cerevisiae. EMBO J 14 4365 373 Stansfield 1, Eurwilaichitr L, Akhmaloka, Tuite ME (1996) Depletion in the levels of the release factor eRFl causes a reduction in the efficiency of translation termination in yeast. Mol Microbiol 20 1135-1143 Stansfield 1, Kushnirov VV, Jones KM, Tuite ME (1997) A conditional-lethal translation termination defect in a sup45 mutant of the yeast Saccharomyces cerevisiae. Fur J Biochem 245 557-563 Stark H (2002) Three-dimensional electron cryomicroscopy of ribosomes. Curr Protein Pept Sci 3 79-91... 

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