Stationary phase of growth

Sub-cellular fractionation of five strains reveal the same numbers of bands. The distribution of PG activity in sub-cellular organelles was broadly similar in these five strains. PG activity was detected in low-density vesicles, vacuoles and ER fractions in samples harvested during the early exponential phase of growth. However, PG levels were always lower (at least 1.5 fold) than those found in wild type. Cells of the mutants harvested during stationary phase of growth showed that 84% of total intracellular PG activity was located in the vesicle fraction. No intracellular PG activity was found in stationary phase wild type cells. 

The development of large-scale cultures involved transferring cells from stock cultures to a series of two liter Fernbach flasks containing enriched seawater medium. After the early stationary phase of growth had been reached (approximately 15-20 days) each of these cultures were used to innoculate 18 liters of the same medium in 20 liter carboys. Large-scale cultures were grown under continuous light (4300 lux cool white fluorescent) at 27.0° C. 

Cells were harvested by centrifugation after cultures reached the early stationary phase of growth (30-35 days). 

Stationary phase of growth curve 470 Statistical effects... 

The maximum biosurfactant production was verified at pH 7.0 and 8.0. The addition of EDTA and microsalts favored microbial synthesis of surface-active compounds. On the other hand, the addition of yeast extract stimulated cell growth to the detriment of biosurfactant production. The most suitable concentration of commercial sucrose for biosurfactant synthesis was 10 g/L. Biosurfactant production occurred in the late-exponential phase, achieving its maximum value at the early stationary phase of growth. The values of surface tension that we obtained compare favorably with those obtained with commercial synthetic surfactants. 

Child, M., Strike, P., Pickup, R. and Edwards, C. (2002) Salmonella typhimurium displays cyclical patterns of sensitivity to UV-C killing during prolonged incubation in the stationary phase of growth. FEMS Microbiol. Lett. 213, 81-85. 

Fig. 6 Cellular DMSP-to-carbon ratios (mol mol) versus specific growth rates under various nutrient limited conditions. Phaeocystis antarctica Fe limited (Stefels and van Leeuwe 1998) P. globosa P and N limited (Stefels unpublished carbon is calculated from cell volume see Table 1) Emiliania huxleyi, Amphidinium carterae and Thalassiosira pseudonana 1 N-limited chemostats (Keller et al. 1999b) T. pseudonana 2 Fe and CO2 limited (Sunda et al. 2002 carbon is calculated from cell volume see Table 1) T. pseudonana 3 N, P, Si and C02 limited (data are taken from the exponential and early stationary phase of growth, Figs. 2-7 in Bucciarelli and Sunda 2003 carbon is calculated from cell volumes, see Table 1)...

Inoculate (sterile technique) 100 ml of sterile Luria broth (200- to 400-ml flask) with a sample of wild-type Bacillus subtilis obtained from a stock culture slant. Incubate this solution on a shaker at 37°C for 15 to 24 hr (until the culture is densely turbid and is entering the stationary phase of growth). 

For maximal production of extracellular virus by TPA induction of B95-8 or P3F1R-1 cells (see Appendix 3), harvest cells at the stationary phase of growth by centrifugation, resuspend in the same volume of fresh medium containing TPA, and incubate for 10 d without additional medium. 

CBH has been reported to be synthesized constitutively in most intestinal bacteria. However, Hylemon and Stellwag [20] detected a 300-fold increase in total units of activity in B. fragilis after the cells entered the stationary phase of growth. CBH activity was detected in the periplasmic space, membrane fraction and soluble extracts. Hence, the synthesis of this enzyme in B. fragilis appears to be under genetic regulation. 

Cebrian, G., Sagarzazu, N., Pagan, R., Condon, S., and Manas, P. 2007. Heat and pulsed electric field resistance of pigmented and non-pigmented enterotoxigenic strains of Staphylococcus aureus in exponential and stationary phase of growth. International Journal of Food Microbiology 118 304—311. 

Kaneko, Kitamura, and Yamamoto (99) studied the susceptibilities to lysis by crude enzyme from Arthrobacter luteus broth of 26 strains of yeast, representing 18 species and 8 genera, although most species belonged to Saccharomyces and Candida. Each yeast was tested during logarithmic and stationary phase of growth in different media. The... 

---