Topoisomerase inhibitory bioassay

Kelm and Nair detected the presence of parthenolide in the hexane extract of M. salicifolia fruits by HPLC [11]. Parthenolide (1) and a related compound, costunolide (2), had shown topoisomerase I (top-l) inhibitory activity (refer to Bioassay Procedures section, Topoisomerase inhibitory bioassays) against Saccharomyces cerevisiae mutant strains hypersensitive to top- poisons [12] (Table 1). The isolation of parthenolide from M. grandiflora [13, 14, 15] and M sieboldii spp. japonica and M. praecoissima a.r. praecoissima [16] is discussed in other studies as well. Costunolide was isolated from the trunk and root bark ether extracts of M sieboldii [17, 18]. Also, parthenolide and costunolide, were isolated from Tanacetum parthenium Asteraceae [19]. The extraction of these compounds by supercritical CO2 (SFCO2) and near critical propane from M. grandiflora has also been carried out [20]. 

The purpose of this bioassay is to detect topoisomerase I (top-1) and or II (top-II) inhibitory activity in crude extracts or compounds. Saccharomyces... 

---