Resin Column

S-Acetylthiocholine chloride [6050-81-3] M 197,7, m 172-173° The chloride can be purified in the same way as the bromide, and it can be prepared from the iodide. A few milligrams dissolved in H2O can be purified by applying onto a Dowex-1 CL resin column (prepared by washing with N HCl followed by COf— free H2O until the pH is 5.8). After equilibration for lOmin elution is started with CO3—free distilled H2O and... 

FIGURE 4.43 Calibration curves for globular proteins on toyopearl resins. Column 22 mm X 30 cm. Sample Protein standards. Elution 0.06 A1 phosphate buffer, pH 7, in 0.06 A1 KCI. Legend elution volume V column volume. 

FIGURE 4.45 Linear pressure/flow curves for Toyopearl HW-55 resins. Column Toyopearl HW-55F, 30-60 fim, 22 mm X 90 cm Toyopearl H W-55S, 20-40 im. 22 mm X 90 cm. Elution Distilled water. 

Toyopearl resin Column size Linear veiocity (cm/hr) Fiow rate (mi/min)... 

Many operating variables, such as sample volume, flow rate, column length, and temperature, must be considered when performing any separation. The relative importance of these variables for Toyopearl HW-55F resin columns has been specifically evaluated. For example. Fig. 4.47 shows the relationship between column efficiency, or height equivalent of a theoretical plate (HETP),... 

FIGURE 6.9 Epoxy resin. Column Shodex GPC KF-803L x 2, 8 mm i.d. x 300 mm x 2. Eluent THE. Flow rate 1.0 mUmin. Detector Shodex UV (2S4 nm). Column temp. Ambient. Sample 100 /xL each, Epikote 1004, Epikote 1001. 

Filtered broth was passed at 2.5 ml/min through a resin column (2.5 cm diameter, 28 cm length) packed with 150 ml of ion exchange resin Amberlite IRC-50 sodium type (Rohm and Haas Co., U.S.A.). The column was washed with water, eluted with 0.5 N HCI at a flow rate 1.3 ml/min. The eluates were fractionated each 10 ml and tuberactinomycin-N activity was found at fractions No. 45-63 obsarved by ultraviolet absorption method and bioassay. 

DETERMINATION OF THE CAPACITY OF AN ION EXCHANGE RESIN (COLUMN METHOD)... 

Ion exchange column. Prepare the Chelex-100 resin (100- 500 mesh) by digesting it with excess (about 2-3 bed-volumes) of 2M nitric acid at room temperature. Repeat this process twice and then transfer sufficient resin to fill a 1.0 cm diameter column to a depth of 8 cm. Wash the resin column with several bed-volumes of de-ionised water. 

Procedure. Allow the whole of the sample solution (1 L) to flow through the resin column at a rate not exceeding 5 mL min . Wash the column with 250 mL of de-ionised water and reject the washings. Elute the copper(II) ions with 30 mL of 2M nitric acid, place the eluate in a small conical flask (lOOmL, preferably silica) and evaporate carefully to dryness on a hotplate (use a low temperature setting). Dissolve the residue in 1 mL of 0.1 M nitric acid introduced by pipette and then add 9 mL of acetone. Determine copper in the resulting solution using an atomic absorption spectrophotometer which has been calibrated using the standard copper(II) solutions. 

Stirring of the methiodide with the anion exchange resin prior to introduction into the column is necessary, because of the insolubility of this quaternary salt in methanol. For methanol-soluble methiodides, a solution of the salt may be added directly to the methanol-washed resin column. 

Techniques for Measuring U-series Nuclides 1992-2002 Separation of U, Th, Pa, and Ra on a TRU Soec Resin Column ... 

A) Sorption breakthrough curves for U(VI) fouled with Fe(lll) on an anion-exchange resin column. 

Bone Sample wet ashed, spiked with 243Am, and purified by anion exchange resin column, solvent extraction, and electrodeposition a -Spectrometry No data 98% Mclnroy et al. 1985... 

Sediments Sample leached with HNO3/HF, filtered, purified by KL-HDEHP resin columns, solvent extracted, and electrodeposition a -Spectroscopy No data 95-99% Guogang et al. 1998... 

Koide et al. [528,529] determined osmium in seawater by passing the water down an anion exchange resin column, followed by distillation of the osmium tetroxide and detection by resonance ionization mass spectrometry. 

In a procedure [627] employing preconcentration of the metals on a column containing a mixture of Chelex 100 and Pyrex glass powder, the problems associated with swelling of the Chelex 100 were overcome and constant flow rates of sample down the column achieved. The water samples were passed through the resin column and eluted with 100 ml 0.01 M nitric acid, and the eluate was discarded. Trace elements were collected from the column by eluting with 50 ml 4 M nitric acid. The eluate was heated to reduce its volume, transferred to a volumetric flask, and diluted to 10 ml. 

The checkers found that the procedure of Helberger and Lan-terman,2 which avoids the use of an ion-exchange resin, is also satisfactory in case it is regarded as inconvenient to set up the resin column. According to this procedure, after completion of the reaction with sodium sulfite, anhydrous hydrogen chloride is passed into the hot solution to liberate sulfur dioxide. After removal of sulfate as in the described procedure, the undiluted aqueous solution is saturated with hydrogen chloride gas at a temperature below 25°. The precipitated sodium chloride is removed by suction filtration, the filter cake is... 

A high-performance liquid chromatographic method for nalidixic acid on a strong anion-exchange resin column has been reported, using a mobile phase of 0.01 M sodium tetraborate at pH 9.2 and 0.003 M sodium sulfate. The relative retention time for nalidixic acid in the system reported by Sondach and Koch was 0.86 with sulfanilic acid as the standard at... 

Table 1 presents the results of fractionations of the DOM. The result of mass balance calculation of the DOC system shows that more than 55 % of the total DOC was retained by XAD-8 resin column, involving the portions of Ho A and HbN/B, and DOC concentrations of the portion eluted by blackwashing (HoA) accounted for 47.4 % of total DOC, as compared with 26.25 % hydrophilic acids (HiA) of the total DOC. More than 11% of the total DOC passed through two resin columns, indicating that small molecular weight polar components were not absorbed onto by XAD-8 and XAD-4. The fractionation did cause potential loss of organic matter by permanent adsorption onto resin s polymers, which were 8.34 % for the XAD-8 resin and 6.41 % for the XAD-4 resin, respectively. 

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