Repeated fed-batch

Kargi, E., Pamukoglu, M.Y. 2004b. Repeated fed-batch biological treatment of pre-treated landfill leachate by powdered activated carbon addition. Enzyme Microbial Technol 34 422 28. 

Fed-batch production of pyruvic acid [CH3COCOOH] from an engineered strain (Escherichia coli YYC202) was optimized by resorting to ED to prevent potential product inhibition in the bioreactor (Zelic et al., 2004). In this way, by continuous separation of pyruvate from the fermentation medium, high values of the pyruvate-to-glucose molar yield (1.78 mol/mol), volumetric productivity (145 kg m 3day ), and pyruvate concentration (79 kg/m3) were achieved by the repeated fed-batch mode. 

Jo EC, Park HJ, Kim DI, Moon HM (1993b), Repeated fed-batch culture of hybridoma cells in nutrient- fortified high-density medium, Biotechnol. Bioeng. 42 1229-1237. 

Culture techniques can be classified into batch, fed-batch, and continuous operation (Table 2). In batch processes, all the nutrients required for cell growth and product formation are present in the medium prior to cultivation. Oxygen is supplied by aeration. The cessation of growth reflects the exhaustion of the limiting substrate in the medium. For fed-batch processes, the usual fed-batch and the repeated fed-batch operations are listed in Table 2. 

Repeated fed-batch process (cyclic fed-batch process)... 

Batch, fed-batch, repeated fed-batch, and continuous modes of bioreactor operation have been used in SSF processes, although batch processes are by far the most common. Fed-batch or continuous operations that involve the addition of fresh, uninoculated substrate particles require interparticle colonization to occur, which is a relatively slow process, and will lead to bioreactor dynamics very different from those obtained during similar processes in SLF. Despite this, laboratory studies have demonstrated potential advantages of such operation. Abdullah et al. [68] compared batch, fed-batch, and repeated fed-batch culture of Chaetomium cellulolyticum on wheat straw. Under optimal conditions in batch culture, protein production ceased after three days, with a maximum protein level of 12 g per gram of solids. In fed-batch culture fresh straw was added at three-day intervals. Protein productivity was maintained for 12 days at a slowly declining rate. In the repeated fed-batch culture half of the fermenting straw was removed and replaced with fresh straw at three-day intervals. Protein productivity was maintained for 12 days at a steady rate and the protein level reached a maximum level of 14 g per gram of solids. 

The software has been applied to a recombinant protein production plant. The mammalian cell culture is processed in a repeated fed batch mode. The essential aim of a model supported control is to continuously estimate the actual state of the culture and, on this information, to predict its behaviour during the next period up to an... 

Xue XD, Li W, LiZM,Xia YL, YeQ. (2010). Enhanced 1,3-propanediol production by supply of organic acids and repeated fed-batch culture. J Ind Microbiol Biotechnol, 37, 681—687. 

Bae SM, Park YC, Lee TH, Kweon DH, Choi JH, Kim SK, Ryu YW, Seo JH. (2004). Production of xylitol by recombinant Saccharomyces cerevisiae containing xylose reductase gene in repeated fed-batch and cell-recycle fermentations. Enzyme Microb Tech, 35, 545-549. 

Ren, N. Q., et al. (2009). Strategy for enhancing photo-hydrogen production yield by repeated fed-batch cultures. International Journal of Hydrogen Energy, 34, 7579—7584. 

Process strategies for commercial-scale production are fed-batch, repeated fed-batch, or continuous cultures for increased productivity [30,32,83]. L-Lysine production is mainly done in fed-batch processes since high product titers can be... 

SBS550MG AadhE, AldhA, AiclR, Apta-ackA, +pyc Igl-i NaHCOg, 2MN32C03 Glucose Repeated fed-batch complex (tryptone, yeast extract) CO2 sparging (0.2lmin , initial OD qq of 17) 40 1.61 0.8 [56]... 

Park YS, Ohtake H, Fukaya M, Kawamura H, Toda K (1991) Production of high concentration of acetic acid by Acetobacter aceti using a repeated fed-batch culture with cell recycling. Appl Microbiol Biotechnol... 

Immobilized cells Ca alginate beads, repeated fed batch ... 

Haloferax mediterranei ATCC 33500, a member of the Archaea family, was used to produce PHA using a mixture of extruded rice bran (ERB) and extruded cornstarch (ECS) as carbon source (Huang et al. 2006). This strain caimot use native rice bran or cornstarch as C-sources. By employing pH-stat control strategy in a 5-L jar bioreactor using ERB ECS (1 8 g g ) as the major carbon source, the authors obtained a cell concentration of 140 g L , PHA concentration of 77.8 g L, and PHA content of 55.6 wt% in a repeated fed-batch fermentation. Otherwise when ECS was used as the major carbon source, a 62.6 g L cell concentration, 24.2 g PHA concentration, and 38.7 wt% PHA content were achieved. ... 

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