Lysine production

Microorganisms with both the required activities were discovered that surprisingly acted with 100% specificity on the isomer of an unnatural substrate, and that did not further metabolise the lysine product. 

Jetten, M.S.M. Follettie, M.T. Sinskey, A.J. Effect of different levels of aspartokinase on the lysine production by Corynebacterium lactofermentum. Appl. Microbiol. Biotechnol., 43, 76-82 (1995)... 

Nomura et al. (1987a) attempted to minimize product inhibitory effect on the aspartate kinase step in lysine biosynthesis and enhance L-lysine production from Brevibacterium Jlavum QL-5 using a combined ED-F system. However, lysine production was not statistically different from that obtained in diffusion dialysis fermentation and about 20% greater than that achieved during conventional fermentation, thus making practically ineffective such a use of ED. 

Ohnishi J, Katahira R, Mitsuhashi S et al (2005) A novel gnd mutation leading to increased L-lysine production in Corynebacterium glutamicum. FEMS Microbiol Lett 242 265-274... 

Hayashi M, Mizoguchi H, Ohnishi J et al (2006) A leuC mutation leading to increased L-lysine production and rel-independent global expression changes in Corynebacterium glutamicum. Appl Microbiol Biotechnol 72 783-789... 

Sindelar G, Wendisch VF (2007) Improving lysine production by Corynebacterium glutamicum through DNA microarray-based identification of novel target genes. Appl Microbiol Biotechnol 76 677-689... 

WittmannC, Kiefer P, Zelder O (2004) Metabolic fluxes in Corynebacterium glutamicum during lysine production with sucrose as carbon source. Appl Environ Microbiol 70 7277-7287... 

Becker J, Klopprogge C, Herold A et al (2007) Metabolic flux engineering of L-lysine production in Corynebacterium glutamicum-over expression and modification of G6P dehydrogenase. J Biotechnol 132 99-109... 

Becker J, Klopprogge C, Zelder O et al (2005) Amplified expression of fructose 1, 6-bispho-sphatase in Corynebacterium glutamicum increases in vivo flux through the pentose phosphate pathway and lysine production on different carbon sources. Appl Environ Microbiol 71 8587-8596... 

Lysine Glucose Investigation of the effects of a novel gnd mutation on lysine production [30]... 

However, overexpression of the malic enzyme gene did not result in improved lysine production independent from the carbon source tested [134]. 

Kelle R, Hermann T, Bathe B (2005) L-Lysine production. In Eggeling L, Bott M (eds) Handbook of Cornyebacterium glutamicum. CRC Press, Taylor and Francis, Boca Raton... 

Amino Acids Lost (mole/mole) Cystine Lysine Products Formed (mole/mole) ... 

A new gene Idc which encodes lysine decarboxylase was found in addition to the formerly known cadA in Escherichia coli and the enzyme purified from the overexpression strain. The lysine decarboxylase encoded by Idc is constitutively produced by E. coli cells though the cadA encodes an inducible one [9]. It is interesting to know of the existence of this new lysine decarboxylase in lysine-producing Corynebacterium and to investigate the effects of the deletion of the gene on the amounts of L-lysine production. 

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