Rat cortical cells

Oshiro S, Kawahara M, Shirao M, Muramoto K, Kobayashi K, Ishige R, Nozawa K, Hori M, Cai Y, Kitajima S, Kuroda Y (1998) Aluminum taken up by transferrin-independent iron uptake affects the iron metabolism in rat cortical cells. J Biochem 123 42-46... 

Yamamoto, H., Tang, H. (1998). Effects of 2-amino-7-phospho-nohepatanoic acid, melatonin or NG-nitro-L-arginine on cyanide or N-methyl-D-aspartate-induced neurotoxicity in rat cortical cells. Toxicol. Lett. 94 13-18. 

EFFECTS OF ADDITIVES AND COOLING RATES ON CRYOPRESERVATION PROCESS OF RAT CORTICAL CELLS... 

Primary rat cortical cells were prepared from neonatal Sprague-Dawley (SD) rats within 3 days of birth (Primary Cell Co., Ltd.). The dissociated cells contained neurons, glial cells, and fibroblasts. These cells were suspended in the culture medium at 1 X 10 cells/ml solution. The materials that were to be tested as cryoprotectants were then added to the medium. The culture medium consisted of Dulbecco s modified Eagle s medium (DMEM) and 10 vol% fetal bovine serum (FBS). Control dissociated neurons that had not been treated with the cryopreservation were cultured so that they could be compared with the cryopreserved neurons. 

Alzheimer s disease, first because of the chronic inflammation which occurs in this illness, and second because the neurodegeneration associated with Alzheimer s is related to the accumulation of amyloid p peptide. Thus, studies with the 12-LOX inhibitor baicalein found that this compound attenuated both neuronal apoptosis as well as the c-jun protein over-expression induced by amyloid P peptide (25-35) on rat cortical cells. In contrast, neither the broad spectrum LOX inhibitor NDGA nor the 5-LOX inhibitor caffeic acid exhibited cell protecting effects [138]. As for baicalein s mechanism of action, Chang et al. [139] demonstrated that the apoptotic effect of this compound on human Hep G2 cells was induced by mitochondrial dysfunction and Bcl-2 regulation. 

Takadera T, Yumoto H, Tozuka Y, OhyashiM T (2002) Prostaglandin E2 induces caspase-dependent apoptosis in rat cortical cells. Neurosci Lett 317 61-64... 

Muller WEG, Ushijima H, Schroder HC, Forrest IMS, Schatton WFH, RytikPG, Heflher-Lauc M (1993) Cytoprotective effect of NMDA receptor antagonist on prion protein (PrionSc)-induced toxidty in rat cortical cell culture. Eur J Pharmacol 246 261-267... 

A further analysis of the distribution of T3 receptors in rat cortical cells suggests a preferential localization in neurons [26,30]. A similar finding was reported in embryonic and neonatal chick brain [31]. Studies with pure cultures of neurons and astrocytes from embryonic and neonatal chick brain have confirmed the predominant localization of the receptors in neuronal nuclei [32,33]. It is clear that thyroid hormone receptors are not restricted to neuronal cells, but also appear in glial cells [34,35] suggesting that both cell types are potential targets for thyroid hormones. A direct effect of T3 on protein phosphorylation has indeed been demonstrated in neuronal and glial cells derived from rat cortex [36,37]. 

Documented effects In modem medicine Inula britannica is used the same way as Inula helenium (Khodzhimatov 1989). Flavonoids isolated from this species were shown to protect cultured rat cortical cells from cell death caused by oxidative stress (Kim et al. 2002). Results from experiments with mice suggest an aqueous extract from the flowers of Inula britannica p.japonica Kitam. has a preventative effect on autoimmune diabetes by regulating cytokine production (Kobayashi et al. 2002b). The sesquiterpene lactone ergolide has anti-inflammatory activity (Han et al. 2(X)1). 

Marie, D. Marie, I. Barker, J. L. Dual video microscopic imaging of membrane potential and cytosolic calcium of immunoidentifled embryonic rat cortical cells. Methods 2000, 21, 335-347. 

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