Human HL-60 leukemia cells

Inayat-Hussain SH, Annuar BO, Laily BD, Naoyuki T. Altholactone, a novel styryl-lactone induces apoptosis via oxidative stress in human HL-60 leukemia cells. Toxicol Lett 2002 131 153-159. 

Kluza, J., Mazinghien, R., Degardin, K., Lansiaux, A. and Badly, C. 2005. Induction of apoptosis by the plant alkaloid sampangine in human HL-60 leukemia cells is mediated by reactive oxygen species. European Journal of Pharmacology, 525(1-3) 32 0. 

In this study we investigated the activity of three acrylate monomers on HL-60 cells by examining of some functional parameters. Ours results clearly indicate that TEGDMA and Bis-GMA, in a dose-dependent manner, reduces cellular proliferation in HL-60 leukemia cell line. This antiproliferative effect of these monomers seems to depend on a cytostatic activity with a cytotoxic effect only at 0.6 and 0.775 mM concentrations. Moreover, the effect on cell proliferation appears higher than that observed with ATRA. HEMA, on the contrary, do not shows any effect on the cellular proliferation. All monomers induce the differentiation process of HL-60 cell line as indicated by the presence of CL activity which is strictly dependent on the appearance of respiratory burst in human myeloid tumour cell lines. 

Chao, P.D. et al.. Quercetin glucuronides inhibited 2-aminofluorene acetylation in human acute myeloid HL-60 leukemia cells, Phytomedicine, 9, 625,2002. 

Han, S. S. et al., Arsenic trioxide represses constitutive activation of NF-kappaB and COX-2 expression in human acute myeloid leukemia, HL-60, J. Cell. Biochem., 94, 695, 2005. 

Fig. 1. Pertussis toxin-mediated ADP ribosylation of membrane G proteins. Isolated cell membranes (50 ng of protein) from N1E 115 cells (mouse neuroblastoma cell line), N2A cells (mouse neuroblastoma cell line), S49-1 eye cells (S49(-) mutated mouse lymphoma cell line deficient in Ga ), 549 wt cells (wild-type mouse lymphoma cell line), RBL (RBL 2H3 rat basophilic leukemia cell line), GH3 cells (GH3 rat hypophyseal tumor cell line), PC-12 (rat pheochromocytoma cell line), HIT-T15 cells (hamster insulinoma cell line), Y-1 cells (mouse adrenal cortex tumor cell line), 108 cc 15 cells (mouse/rat neuroblastoma x glioma hybrid cell line), HL-60 cells (DMSO-differentiated human leukemia cell line), HL-60 (+PT) cells (HL-60 cells pretreated with 25 ng/ml of pertussis toxin for 24 h prior to preparation of membranes), RINm5F cells (rat insulinoma cell line), and C6-2 cells (rat glioma cell line) were subjected to P-ADP-ribosylation as described in section 4.3.3. Samples were precipitated as outlined in section 4.3.5 and subjected to SDS-PAGE with separating gels containing 8% acrylamide (w/v). An autoradiogram of the dried gel is shown. Molecular masses of marker proteins are indicated (kDa). Modified Ga proteins migrate at approximately 40 kDa. Radioactivity running in front of the 30 kDa marker protein comigrates with the dye front...

Vinca alkaloids are lipophilic molecules that can readily cross membranes by simple diffusion [186]. Experiments performed with several human cancer and tissue cell lines have shown in all cases rapid uptake of every one of Vinca alkaloids [23, 187-189]. Uptake is thought to occur by diffusion although energy dependence or independence of the uptake is seldom mentioned in reports. However, for instance in cultured human promyelocytic leukemia HL-60/C1 cells it has been shown that rates of uptake of vinblastine were unaffected by depletion of cellular adenosine triphosphate, reinforcing that uptake is not mediated by an energy-dependent system [189]. 

The recent demonstration that l,25-(OH)2D stimulates the differentiation of myeloid leukemia cells (MI and human HL-60 cells) into monocytes in a dose-dependent manner, 02,103suggests a potential role for vitamin D metabolites in cellular differentiation. Somewhat higher doses of l,25-(OH)2D2 are required than those effective in cultures of embryonic bone or chick embryonic Intestine, but the compounds that show maximum effectiveness in terms of calcium action also show maximum effect in stimulating the differentiation process. 

C.sHiiOj, Mr 276.28, oil, [a]g 0° (CHClj). N. is an inducer of differentiation of human HL-60 promyelocy tic leukemia cells. It was isolated from fermentations of the basidiomycete Nidula Candida, together with niduloic acid CuHieO, Mr 276.28, yellow oil, [d -53° (CHCI3). The name niduloic acid is also used for (-H)-3-hydroxy-5-(4-hydroxyphenyl)pentanoic acid. Ut. J. Antibiot. 49, 1189-1195 (1996) (isolation). -[CAS I85853-I4-9 (N.) ]85853-15-0 (niduloic acid)]... 

HL-60 human acute myeloid leukemia cell line... 

Nojiri, H., Takaku, H., Tetsuka, T, Motoyoshi, K., Miura, Y, and Saito, M., 1984, Characteristic expression of glycosphingolipid profiles in the bipotential cell differentiation of human promy-elocytic leukemia cell line HL-60, Blood 64 534-541. 

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