Polymerase chain reaction capillary

Garcia-Canas, V., Macian, M. C., Chenoll, E., Aznar, R., Gonzalez, R., and Cifuentes, A., Detection and differentiation of several food-spoilage lactic acid bacteria by multiplex polymerase chain reaction, capillary gel electrophoresis, and laser-induced fluorescence, J. Agric. Food Chem., 52, 5583, 2004. 

Baba, Y., Tomisaki, R., Sumita, C., Morimoto, I., Sugita, S., Tsuhako, M., Miki, T., and Ogihara, T., Rapid typing of variable number of tandem repeat locus in the human apolipoprotein B gene for DNA diagnosis of heart disease by polymerase chain reaction and capillary electrophoresis, Electrophoresis, 16, 1437, 1995. 

Analysis of polymerase chain reaction-product by capillary electrophoresis with laser-induced fluorescence detection and its application to the diagnosis of medium-chain acyl-coenzyme A dehydrogenase deficiency. 

A thermal cycler (see 8.2.3.2 Polymerase Chain Reaction) is required for the cycle sequencing reaction. For fluorescent cycle sequencing we recommend instruments from Applied Biosystems (e.g. the 16-capillary ABI PRISM 3100 Genetic Analyser) other instruments are available from GC Healthcare and Beckman. For detailed instructions, refer to the respective user s manual or chemistry guide. 

Zhou et al. [175] described the determination of severe acute respiratory syndrome (SARS) coronavirus by a microfluidic chip system. The unit included an LIF microfluidic chip analyzer, a glass microchip for both PCR and capillary electrophoresis, a chip thermal cycler based on dual Peltier thermoelectric elements, a reverse transcription-polymerase chain reaction (RT-PCR) SARS diagnostic kit, and a DNA electrophoretic sizing kit. According to the authors, the system allowed efficient DNA amplification of the SARS coronavirus followed by electrophoretic sizing and detection on the same chip. 

Cheng, J., Waters, L.C., Fortina, P., Hvichia, G., Jacobson, S.C., Ramsey, J.M., Kricka, L.J., Wilding, P., Degenerate oligonucleotide primed-polymerase chain reaction and capillary electrophoretic analysis of human DNA on microchip-based devices. Anal. Biochem. 1998, 257(2), 101-106. 

The excellent resolving power of gel or polymer solution-filled narrow-bore channels readily provides sequencing-grade separation of very similar size DNA molecules, usually detected by laser-induced fluorescence [9]. Collection of any individual or all separated fragments by capillary electrophoresis (CE) proved to be feasible and provided enough material for such downstream processes as polymerase chain reaction (PCR), sequencing, or cloning [10-12],... 

Bianchi N, Mischiati C, Feriotto G, Gambari R (1994) Detection of hepatitis C virus by unbalanced polymerase-chain reaction, hybridization to synthetic oligonucleotides and capillary electrophoresis. Int J Oncol 4 903-907. 

Butler JM, McCord BR, Jung JM, Wilson MR, Budowle B, Allen RO (1994) Quantitation of polymerase chain reaction products by capillary electrophoresis using laser fluorescence. J Chromatogr B 658 271-280. 

Fasco MJ, Treanor CP, Spivack S, Figge HL, Kaminsky LS (1995) Quantitative RNA-polymerase chain reaction—DNA analysis by capillary electrophoresis and laser induced fluorescence. Anal Biochem 224 140-147. 

McCord BR, McClure DL, Jung JM (1993) Capillary electrophoresis of polymerase chain reaction-amplified DNA using fluorescence detection with an intercalating dye. J Chromatogr 652 75-82. 

Srinivasan K, Girard JE, Williams P, Roby RK, Weedn VW, Morris SC, Kline MC, Reeder DJ (1993a) Electrophoretic separations of polymerase chain reaction-amplified DNA fragments in DNA typing using a capillary electrophoresis-laser induced fluorescence system. / Chromatogr 652 83-91. 

Electrophoretic separation lies at the heart of polymerase chain reaction (PCR), isoelectric focusing (lEF), 2D gel electrophoresis, pulsed-field electrophoresis, capillary electrophoresis, and immunoelectrophoresis. 

Polymerase Chain Reaction Products Analysis Using Capillary Electroph Mark P. Richards... 

Figure 39-7 Polymerase chain reaction (PCR) analysis for clonal rearrangements of the antigen-receptor gene loci. PCR analysis of theTCR y-chain locus is shown. The upper and middle panels show duplicate assays with monoclonal capillary electrophoretic peaks of identical size ( 166 bp) in both replicates.The bottom panel shows a polyclonal pattern.

Different protein-based methods have been reviewed for species identification in milk and dairy products, and for characterization of cheese maturity, such as electrophoretic, chromatographic, and immunological techniques (11, 12). In addition to new developments in these techniques, the interdisciplinary and dynamic nature of milk product analysis is being enhanced by the application of disciplines already used to analyze other foodstuffs. Among them, capillary electrophoresis (CE), polymerase chain reaction, and isotope ratio mass spectrometry are just gaining popularity for solving dairy authenticity problems (13-15). 

Bjorheim J, Lystad S, Lindblom A, et al. Mutation analyses of KRAS exon 1 comparing three different techniques temporal temperature gradient electrophoresis, constant denaturant capillary electrophoresis and allele specific polymerase chain reaction. MutatRes. 1998 403 103-112. 

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