Polymerase chain reaction multiplex

Due to the low specificity of culture and clinical diagnosis, the multiplex polymerase chain reaction has been employed successfully in diagnosing Haemophilus ducreyi. 

Danik M, Puma C, Quirion R, et al. Widely expressed transcripts for chemokine receptor CXCR1 in identified glutamatergic, gamma-aminobutyric acidergic, and cholinergic neurons and astrocytes of the rat brain a single-cell reverse transcription-multiplex polymerase chain reaction study. J Neurosci Res 2003 74 286-295. 

Bej, A. K. McCarty, S. C. Atlas, R. M. Detection of coliform bacteria and Escherichia coli by multiplex polymerase chain reaction comparison with defined substrate and plating methods for water quality monitoring. Appl. Environ. Microbiol. 1991, 57, 2429-2432. 

B5. Berg, C., Hedrum, A., Holmberg, A., Ponten, F., Uhler, M. et al., Direct solid-phase sequence analysis of the human p53 gene by use of multiplex polymerase chain reaction and alpha-thiotriphosphate nucleotides. Clin. Chem. (Winston-Salem, N.C.) 41, 1461-1466 (1995). 

Bluhm, B. H., Flaherty, J. E., Cousin, M. A., and Woloshuk, C. P. (2002). Multiplex Polymerase Chain Reaction assay for the differential detection of trichothecene- and fumonisin-producing species of Fusarium in cornmeal. /. Food Prot. 65,1955-1961. 

Chen, R. S., Tsay, J. G., Huang, Y. F., and Chiou, R. Y. Y. (2002). Polymerase chain reaction-mediated characterization of molds belonging to the Aspergillus flavus group and detection of Aspergillus parasiticus in peanut kernels by a multiplex polymerase chain reaction. /. Food Prot. 65, 840-844. 

Dean, T. R., Roop, B., Betancourt, D., and Menetrez, M. Y. (2005). A simple multiplex polymerase chain reaction assay for the identification of four environmentally relevant fungal contaminants. /. Microbiol. Methods 61, 9-16. 

Geisen, R. (1996). Multiplex polymerase chain reaction for the detection of potential aflatoxin and sterigmatocystin producing fungi. Syst. Appl. Microbiol. 19, 388-392. 

Charbonnier F, Raux G, Wang Q, Drouot N, Cordier F, Limacher JM, Saurin JC, Puisieux A, Olschwang S, Frebourg T. Detection of exon deletions and duplications of the mismatch repair genes in hereditary nonpolyposis colorectal cancer families using multiplex polymerase chain reaction of short fluorescent fragments. Cancer Res 2000 60(ll) 2760-2763. 

Since alterations in global DNA methylation are implicated in various pathobio-logical processes, a gradient IPC-ESI-MS/MS method with a volatile IPR was used to determine cytosine and 5-methylcytosine in DNA quantification relied on stable isotope dilution [58], Muscular dystrophies caused by various mutations in the dystrophin gene are amenable to easier prenatal diagnosis via a multiplex polymerase chain reaction (PCR)/IPC assay [59]. Some guidelines for the analysis of genomic DNA by IPC-ESl-MS can be found in Reference 60. 

Hopkins KL, Hilton AC (2000) Simultaneous molecular subtyping and shiga toxin gene detection in Escherichia coli using multiplex polymerase chain reaction. Lett Appl Microbiol 30 122-125... 

Berfstrome Jones AC, Doon A. Evaluation of a single-tube multiplex polymerase chain reaction screen for detection of common alpha thalassemia genotypes in a clinical laboratory Am J Clin Path 2002 118 18-24. 

Bowie LJ, Reddy PL, Nagabhushan M, Sevigny P. Detection of a thalassemia by multiplex polymerase chain reaction. Clin Chem 1994 40 2260-6. 

Dubreuil Lastmcci RM, Dawson DA, Bowden JH, Munster M. Development of a simple multiplex polymerase chain reaction for the simultaneous detection of factor V Leiden and prothrombin 202lOA mutations. Molecular Diag 1999 4 247-50. 

Stuven T, Griese EU, Kroemer HK, Eichelbaum M, Zanger UM. Rapid detection of CYP2D6 null alleles by long distance- and multiplex-polymerase chain reaction. Pharmacogenetics 1996 6 417-21. 

Fritz, A., M. Rozowski, C. Walker and M. Westerfield. Identification of selected gamma-ray induced deficiencies in zebrafish using multiplex polymerase chain reaction. Genetics 144 1735-1745, 1996. 

The HuSNP experiments were conducted according to the GeneChip HuSNP Mapping Assay Manual (P/N 7(X)308, Affymetrix, INC., Santa Clara, CA). Genomic DNA (120 ng) or cDNA (6 ng) was used for each set of 24 multiplex polymerase chain reactions (PCRs see Notes 1 and 2). 

Freeman, B., Smith, N., Curtis, C., Huckett, L., Mill, J., and Craig, I. W. (2003) DNA from buccal swabs recruited by mail evaluation of storage effects on longterm stability and suitability for multiplex polymerase chain reaction genotyping. Beh. Genet. 33,67-72. 

Garcia-Canas, V., Macian, M. C., Chenoll, E., Aznar, R., Gonzalez, R., and Cifuentes, A., Detection and differentiation of several food-spoilage lactic acid bacteria by multiplex polymerase chain reaction, capillary gel electrophoresis, and laser-induced fluorescence, J. Agric. Food Chem., 52, 5583, 2004. 

Virtually all the forensic DNA analyses performed worldwide are multiplex polymerase chain reaction... 

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